Team:Clemson

From 2014.igem.org

(Difference between revisions)
(→Welcome to the Official Page of Clemson iGEM!)
 
(44 intermediate revisions not shown)
Line 1: Line 1:
-
{{Team:Clemson/page-header}}
+
{{Template:Clemson}}
-
=Welcome to the Official Page of Clemson iGEM!=
+
<html>
-
<center><a href="https://static.igem.org/mediawiki/2013/c/c7/TillmanPaw.png" width="600px" height="400px"/></a></center>
+
<!--main content -->
 +
<table width="70%" align="center">
 +
</html>
-
==Our Mission==
+
[[File:bumpdd.jpg|500px|frameless|center]]
-
The FDA has maintained a zero-tolerance policy for several foodborne pathogens.  For example, a policy of “zero-tolerance” for ''Listeria monocytogenes'' in ready-to-eat foods means that the detection of any ''L. monocytogenes'' in either of two 25 gram samples of a food renders the food adulterated; the infectious dosage of ''E. coli'' O157:H7 has been determined to be 10 cells; the Environmental Protection Agency standard for ''E. coli'' O157:H7 in water is 40 cells per liter.  The current detection methods suffer from one or more of the following limitations: 1) the requirement of pre-enrichment and enrichment to increase the number of target pathogens, e.g., bio-chemical assays and immunoassays, 2) high detection limit, e.g., 10^3 – 10^5 CFU per ml or per gram of sample for immunoassays, 3) inability to distinguish viable from non-viable cells, e.g., PCR-based detection methods, 4) small sample volume capacity, e.g., microfluidic-based biosensors (µl instead of the required ml to liter capacity), 5) tedious detection procedures, and 6) the current high per-assay cost.
+
<html>
 +
<tr>
-
The aim of this project is develop a Universal Self-Amplified (USA) Biosensor that addresses the aforementioned disadvantages of current detection methods.  This two component system utilizes a universal signal amplification bacterial system and a unique pathogen-specific detection counterpart for a one-step detection of target microorganisms in a scalable volume.
 
-
{{Team:Clemson/page-footer}}
+
 
 +
 
 +
 
 +
 
 +
</td>
 +
 
 +
<tr> <td colspan="3"  height="15px"> </td></tr>
 +
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
 +
<tr> <td colspan="3"  height="5px"> </td></tr>
 +
 
 +
 
 +
<!--requirements section -->
 +
<tr><td colspan="3"> <h3> Quorum Sensing</h3></td></tr>
 +
<tr>
 +
<td width="45%"  valign="top">
 +
 
 +
</html>
 +
[[File:squid.jpg|500px|frameless|center]]
 +
 
 +
<br>
 +
 
 +
[[File:glowy.jpg|500px|frameless|center]]
 +
<html>
 +
 
 +
</ul>
 +
 
 +
</td>
 +
 
 +
<td > </td>
 +
<td width="45%">
 +
 
 +
<p>Quorum sensing is a means for bacteria to communicate with one another. This signaling is density-dependent, and when a large enough number of organisms are present, they even coordinate gene expression. A famous example is Vibrio fischeri, which gives the Hawaiian bobtail squid its eerie glow. When a large enough concentration of Vibrio fischeri come together in the squid's light-producing organ, or photophore, they communicate by quorum sensing and all express the luciferase gene, resulting in beautiful bioluminescence.
 +
 
 +
Quorum sensing is largely dependent upon the production of N-acyl Homoserine Lactones or AHLs. The 2014 Clemson iGEM Team worked to develop a system to detect the presence of AHLs for better study of quorum sensing and biofilm interactions. This system can be readily adapted to detect any number molecules and thus provide a flexible tool for microbiologists.  </p>
 +
 
 +
</td>
 +
</tr>
 +
 
 +
 
 +
<tr> <td colspan="3"  height="15px"> </td></tr>
 +
<tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>

Latest revision as of 03:08, 18 October 2014

Bumpdd.jpg


Retrieved from "http://2014.igem.org/Team:Clemson"

Quorum Sensing

Squid.jpg


Glowy.jpg

Quorum sensing is a means for bacteria to communicate with one another. This signaling is density-dependent, and when a large enough number of organisms are present, they even coordinate gene expression. A famous example is Vibrio fischeri, which gives the Hawaiian bobtail squid its eerie glow. When a large enough concentration of Vibrio fischeri come together in the squid's light-producing organ, or photophore, they communicate by quorum sensing and all express the luciferase gene, resulting in beautiful bioluminescence. Quorum sensing is largely dependent upon the production of N-acyl Homoserine Lactones or AHLs. The 2014 Clemson iGEM Team worked to develop a system to detect the presence of AHLs for better study of quorum sensing and biofilm interactions. This system can be readily adapted to detect any number molecules and thus provide a flexible tool for microbiologists.