Team:Clemson

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=Welcome to the Official Page of Clemson iGEM!=
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=Welcome to the Official Page of Clemson iGEM!=
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==Our Mission==
 
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The FDA has maintained a zero-tolerance policy for several foodborne pathogens.  For example, a policy of “zero-tolerance” for ''Listeria monocytogenes'' in ready-to-eat foods means that the detection of any ''L. monocytogenes'' in either of two 25 gram samples of a food renders the food adulterated; the infectious dosage of ''E. coli'' O157:H7 has been determined to be 10 cells; the Environmental Protection Agency standard for ''E. coli'' O157:H7 in water is 40 cells per liter.  The current detection methods suffer from one or more of the following limitations: 1) the requirement of pre-enrichment and enrichment to increase the number of target pathogens, e.g., bio-chemical assays and immunoassays, 2) high detection limit, e.g., 10^3 – 10^5 CFU per ml or per gram of sample for immunoassays, 3) inability to distinguish viable from non-viable cells, e.g., PCR-based detection methods, 4) small sample volume capacity, e.g., microfluidic-based biosensors (µl instead of the required ml to liter capacity), 5) tedious detection procedures, and 6) the current high per-assay cost.
 
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The aim of this project is develop a Universal Self-Amplified (USA) Biosensor that addresses the aforementioned disadvantages of current detection methods.  This two component system utilizes a universal signal amplification bacterial system and a unique pathogen-specific detection counterpart for a one-step detection of target microorganisms in a scalable volume.
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<tr><td colspan="3"> <h3> Quorum Sensing</h3></td></tr>
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<p>Quorum sensing is a means for bacteria to communicate with one another. This signaling is density-dependent, and when a large enough number of organisms are present, they even coordinate gene expression. A famous example is Vibrio fischeri, which gives the Hawaiian bobtail squid its eerie glow. When a large enough concentration of Vibrio fischeri come together in the squid's light-producing organ, or photophore, they communicate by quorum sensing and all express the luciferase gene, resulting in beautiful bioluminescence.
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Quorum sensing is largely dependent upon the production of N-acyl Homoserine Lactones or AHLs. The 2014 Clemson iGEM Team worked to develop a system to detect the presence of AHLs for better study of quorum sensing and biofilm interactions. This system can be readily adapted to detect any number molecules and thus provide a flexible tool for microbiologists.  </p>
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Latest revision as of 03:08, 18 October 2014

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Retrieved from "http://2014.igem.org/Team:Clemson"

Quorum Sensing

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Quorum sensing is a means for bacteria to communicate with one another. This signaling is density-dependent, and when a large enough number of organisms are present, they even coordinate gene expression. A famous example is Vibrio fischeri, which gives the Hawaiian bobtail squid its eerie glow. When a large enough concentration of Vibrio fischeri come together in the squid's light-producing organ, or photophore, they communicate by quorum sensing and all express the luciferase gene, resulting in beautiful bioluminescence. Quorum sensing is largely dependent upon the production of N-acyl Homoserine Lactones or AHLs. The 2014 Clemson iGEM Team worked to develop a system to detect the presence of AHLs for better study of quorum sensing and biofilm interactions. This system can be readily adapted to detect any number molecules and thus provide a flexible tool for microbiologists.