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Team:BNU-China/home.html
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<h2>Our work</h2> | <h2>Our work</h2> | ||
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| - | <p>The ability of rhizobium to fix nitrogen is essential for legumes growth and development. The stronger the ability, the less chemical fertilizer will be used. Molybdenum is a key element in rhizobial nitrogen fixing enzyme, and is also necessary for the growth of plants themselves. However, Mo being heavy mental, abusing it will pollute the environment. Hence, spot application of Mo is of great importance; it would be even better to concentrate the element only around the roots. | + | <p>The ability of rhizobium to fix nitrogen is essential for legumes growth and development. The stronger the ability, the less chemical fertilizer will be used. Molybdenum is a key element in rhizobial nitrogen fixing enzyme, and is also necessary for the growth of plants themselves. However, Mo being heavy mental, abusing it will pollute the environment. Hence, spot application of Mo is of great importance; it would be even better to concentrate the element only around the roots. <p/> |
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| - | We transferred our designed plasmids into E.coli "Prometheus" to make it express a fusion protein: one half of it anchors on the outter membrane and the other side, namely ModA,absorbs molybdate. With the help of organic acid chemotaxis system, our "Prometheus" will head for the roots. A suicide system depending on time is also designed for bio-safety concerns. E.coli Prometheus will deliver Mo directly to the root. This opens a new chapter of biological fertilizer. | + | <p>We transferred our designed plasmids into <i>E.coli<i/> "Prometheus" to make it express a fusion protein: one half of it anchors on the outter membrane and the other side, namely ModA, absorbs molybdate. With the help of organic acid chemotaxis system, our "<i>Prometheus<i/>" will head for the roots. A suicide system depending on time is also designed for bio-safety concerns. <i>E.coli Prometheus<i/> will deliver Mo directly to the root. This opens a new chapter of biological fertilizer. |
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Revision as of 03:04, 18 October 2014
Maps
Our work
The ability of rhizobium to fix nitrogen is essential for legumes growth and development. The stronger the ability, the less chemical fertilizer will be used. Molybdenum is a key element in rhizobial nitrogen fixing enzyme, and is also necessary for the growth of plants themselves. However, Mo being heavy mental, abusing it will pollute the environment. Hence, spot application of Mo is of great importance; it would be even better to concentrate the element only around the roots.
We transferred our designed plasmids into E.coli "Prometheus" to make it express a fusion protein: one half of it anchors on the outter membrane and the other side, namely ModA, absorbs molybdate. With the help of organic acid chemotaxis system, our "Prometheus" will head for the roots. A suicide system depending on time is also designed for bio-safety concerns. E.coli Prometheus will deliver Mo directly to the root. This opens a new chapter of biological fertilizer.
Wiki Design
Art design, pictures, figures and the latest experiment results were put on the wiki in time. Wiki is an important platform to show our works.
ModA & INPN
ModA we used in our project is a kind of protein that can bind the molybdate and INPN can display ModA on E.coli's surface.
Chemotaxis
We Used McfR plasmid to give the E.coli the capacity of Chemotaxis, so that those E.coli with molybdate can swim to the root of peanut.
Modeling
We have made the model of peanut root and E.coli in environment to show how our thought be realized out of lab.
Latest Works
Our Sponsors
The Story of E.coli Prometheus
BNU-China
