Team:UT-Tokyo/CTCD/Content

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<li id="Result"><a href="Javascript:open('Result')"><img src="https://static.igem.org/mediawiki/2014/1/11/LinkCTC_resulton.png" class="contList" alt="https://static.igem.org/mediawiki/2014/8/83/LinkCTC_resultopen.png"></a>
<li id="Result"><a href="Javascript:open('Result')"><img src="https://static.igem.org/mediawiki/2014/1/11/LinkCTC_resulton.png" class="contList" alt="https://static.igem.org/mediawiki/2014/8/83/LinkCTC_resultopen.png"></a>
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<li><a href="#">Contents1</a></li>
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<li><a href="Javascript:loadContent('Result-block','Result-1')">miRNA-binding sites</a></li>
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<li><a href="#">Contents2</a></li>
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<li id="Lab"><a href="Javascript:open('Lab')"><img src="https://static.igem.org/mediawiki/2014/6/6d/LinkCTC_labon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/d/de/LinkCTC_labopen.png"></a>
<li id="Lab"><a href="Javascript:open('Lab')"><img src="https://static.igem.org/mediawiki/2014/6/6d/LinkCTC_labon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/d/de/LinkCTC_labopen.png"></a>
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<li><a href="Javascript:loadContent('Lab-block','Lab-1')">Parts</a></li>
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<li><a href="Javascript:loadContent('Lab-block','Lab-2')">Protocol</a></li>
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<li><a href="Javascript:loadContent('Lab-block','Lab-3')">Lab Note</a></li>
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<li id="Modeling"><a href="Javascript:open('Modeling')"><img src="https://static.igem.org/mediawiki/2014/4/42/LinkCTC_modelingon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/5/56/LinkCTC_modelingopen.png"></a>
<li id="Modeling"><a href="Javascript:open('Modeling')"><img src="https://static.igem.org/mediawiki/2014/4/42/LinkCTC_modelingon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/5/56/LinkCTC_modelingopen.png"></a>
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<li><a href="Javascript:loadContent('Modeling-block','Modeling-1')">BBa_K747096</a></li>
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<li id="Achieve"><a href="Javascript:open('Achieve')"><img src="https://static.igem.org/mediawiki/2014/8/88/LinkCTC_achieon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/4/41/LinkCTC_achieopen.png"></a>
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<li id="Achievement"><a href="Javascript:open('Achievement')"><img src="https://static.igem.org/mediawiki/2014/8/88/LinkCTC_achieon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/4/41/LinkCTC_achieopen.png"></a>
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<li><a href="Javascript:loadContent('Achievement-block','Achievement-1')">Judging Form</a></li>
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<li id="Safety"><a href="Javascript:open('Safety')"><img src="https://static.igem.org/mediawiki/2014/9/96/LinkCTC_safetyon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/9/93/LinkCTC_safetyopen.png"></a>
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<li id="Safety"><a href="Javascript:loadContent('Safety-block','top')"><img src="https://static.igem.org/mediawiki/2014/9/96/LinkCTC_safetyon.png" class="contList"></a>
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<li id="Attribution"><a href="Javascript:open('Attribution')"><img src="https://static.igem.org/mediawiki/2014/3/38/LinkCTC_attributionon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/9/92/LinkCTC_attributionopen.png"></a>
<li id="Attribution"><a href="Javascript:open('Attribution')"><img src="https://static.igem.org/mediawiki/2014/3/38/LinkCTC_attributionon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/9/92/LinkCTC_attributionopen.png"></a>
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<li><a href="Javascript:loadContent('Attribution-block','Attribution-1')">Attribution</a></li>
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<li><a href="Javascript:loadContent('Attribution-block','Attribution-2')">Acknowledgement</a></li>
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<li><a href="Javascript:loadContent('Attribution-block','Attribution-3')">Sponsors</a></li>
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<li id="Humanpractice"><a href="Javascript:open('Humanpractice')"><img src="https://static.igem.org/mediawiki/2014/5/5e/LinkCTC_humanon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/c/c9/LinkCTC_humanopen.png"></a>
<li id="Humanpractice"><a href="Javascript:open('Humanpractice')"><img src="https://static.igem.org/mediawiki/2014/5/5e/LinkCTC_humanon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/c/c9/LinkCTC_humanopen.png"></a>
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<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-1')">Human Practice</a></li>
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<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-2')">Ethics</a></li>
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<li id="Team"><a href="Javascript:loadContent('Team-top','top')"><img src="https://static.igem.org/mediawiki/2014/8/83/LinkCTC_teamon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/3/31/LinkCTC_teamopen.png"></a>
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<img src="https://static.igem.org/mediawiki/2014/d/d9/SubCTC_introduction.png" class = "contTitle" />
 
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<p>Please imagine that you are a police officer. You are searching for dangerous criminals who hide in crowd and it is difficult to find them. This situation is the same as finding cancer cells (dangerous criminals in our body) in our blood when they resemble to normal blood cells. In this way picking up cancer cells has been developed around the world, where every year a different method is developed. Don't you think that it is like a dream if there were drugs, which if someone take, they will confess their crime? What about drugs, which if the cancer cells take, they will shine brightly on the blood? Here our team focuses on microRNA (miRNA) and provides a special genetic circuit which exposes cancer cells in blood vessels.(Fig.1)</p>
 
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<img src="https://static.igem.org/mediawiki/2014/f/fa/Introduction_CTC_fig1.png" class ="figure" />
 
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<p>Circulating tumor cells (CTCs) tumor cells arising from epithelia, flowing through blood vessels and causing metastasis to other tissues. Focusing on these cells, there are ways of early detection of cancer, detecting CTCs in blood of patients with early-stage cancer. The effectiveness of these methods has been ensured through the results of researches especially in breast cancer and colon cancer (ref). These methods of detection are a technology developed in recent years, compared to the traditional methods of picking up the tissue of patients which has been used for a long time. However, these traditional methods produce a hard burden to the patients, because of the several and long procedures. On the other hand, the methods of detecting CTCs produce a non-invasive option for patients compared to traditional ones. From the utility points, CTCs have been used all around the world to develop several detection methods. Most of these methods emphasized utilizing anti-EpCAM antibody, which is an adhesion protein used as marker of cancers, and distinguish CTCs from blood cells by the difference of their cell size. However, because the concentration of CTCs in blood is very low, it is required to develop a new approach for the detection of CTCs.</p>
 
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<p>Here we have developed a new detection method for CTCs focusing on two types of profiles; miRNA and EpCAM. It has given the more specificity for CTCs to utilize the two profiles. miRNA is a special short non-cording RNA and interferes the translation of the complementary mRNA, interacting with some proteins and forming the RNA-induced silencing complex (RISC) in wide-species from virus to human. Focusing on different expression patterns of miRNA in each cell type, we utilized miRNA profiles with a synthetic biological approach for detecting CTCs. The circuit that we have developed has EpCAM promoter, reporter and blood cells-specific miRNA (<i>miR-142-3p/5p</i>) binding sites. Two conditions, which are activation of EpCAM promoter and an absence of blood cells-specific miRNA, are required for translation of the reporter. If we inject this circuit into cells in blood, only in the case of CTCs the reporter is produced.(Fig.2)</p>
 
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<img src="https://static.igem.org/mediawiki/2014/1/13/Introduction_CTC_fig2.png" class ="figure" />
 
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<p>[1] Friedlander, Terence W., Gayatri Premasekharan, and Pamela L. Paris. "Looking back, to the future of circulating tumor cells." <i>Pharmacology & therapeutics</i> 142.3 (2014): 271-280.</p>
 
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<img src = "https://static.igem.org/mediawiki/2014/8/8f/SubCTC_system.png" class = "contTitle"/>
 
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<img src="https://static.igem.org/mediawiki/2014/6/64/Kobari_pegp2.png" class = "figure" />
 
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<legend>Fig.3 the construct of our circuit</legend>
 
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<h3>EGP-2 promoter</h3>
 
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<p>EGP-2 promoter is the promoter of Epithelial cell adhesion molecule (EpCAM) protein.EpCAM is a trans membrane glycoprotein which is expressed in epithelial cells and shows high level expression in various type of human epithelial carcinomas[2].Using this promoter, reporter genes can be expressed only in cancer cells. EGP-2 promoter is originally about 3500bp and it is not convenient to make a DNA construct. But, in the previous research, it is known that EGP-2 promoter can work  if a part of its sequence is deleted[3]. In this project ,We cloned a part of EGP-2 promoter, and made it useful as a biobrick parts.</p>
 
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<h3>miRNA-142</h3>
 
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<p>MiRNA is a class of non-coding RNA.MiRNA binds the miRNA-recognition element in the 3' untranslated region of the target gene. MiRNA shows the tissue-specific expression pattern. In our project, we use miRNA-142, which is expressed only in hematopoietic cells[4].After miRNA-142 is transcribed, it is cleaved to miRNA-142-5p and miRNA-142-3p.  Adding the recognition element of miRNA-142 in the 3' untranslated region of the reporter genes, leaky expression in non-carcinoma hematopoietic cells can be reduced.</p>
 
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<img src="https://static.igem.org/mediawiki/2014/9/94/Kobari_CTC.png" class = "figure" />
 
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<legend>Fig.4 When this construct is transfected to the epithelial cells like cancer cell, pEGP-2 promote the transcription of EGFP, and the mRNA is not degraded by miRNA because miR 142 is specific to hematopoietic cells.</legend>
 
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<img src="https://static.igem.org/mediawiki/2014/4/44/Kobari_hematopoietic_cell.png" class = "figure" />
 
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<legend>Fig.5 When this construct is transfected to the hematopoietic cells, pEGP-2 does not work as a promoter, and mRNA is not transcribed. Even if the leak of the promoter makes few mRNA ,this mRNA is degraded by RNAi caused by miR 142.</legend>
 
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<img src = "https://static.igem.org/mediawiki/2014/b/b4/SubCTC_application.png" class = "contTitle" />
 
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<p>In our project, miRNA is used for degrading mRNA in cells that express miRNA. But if we transfect construct like [pCMV-LacI-miR A binding site] and [pCAG-LacO2-GFP][5], mRNA in cells that does not express miRNA is degraded. This system will makes many application of miRNA in iGEM possible.</p>
 
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<p>We focused on a tissue-specific promoter and miRNA in order to detect CTCs.As well as hematopoietic cells, many other tissues shows specific miRNA expression patterns[3]. Therefore, if by making a circuit that returns an output to a certain miRNA expression pattern, we may identify where a CTC comes from.[5]</p>
 
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<p>In addition to the detection of CTCs, combinations of tissue-specific promoter and miRNA can be applied to many treatments. For example, suicide genes can be expressed in cancer cells in a specific tissue.[6]</p>
 
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<div class = "member">
<div class = "member">
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<p class = "name">MASAKI ONO</p>
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<p class = "name">YOICHI IRIE</p>
<img src = "https://static.igem.org/mediawiki/2014/6/6a/Irie_comb.png" class ="member-detail" />
<img src = "https://static.igem.org/mediawiki/2014/6/6a/Irie_comb.png" class ="member-detail" />
<dl class = "profile">
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<dt>Name</dt>
<dt>Name</dt>
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<dd>Masaki Ono</dd>
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<dd>Yoichi Irie</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
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<dd>The University of Tokyo, Literature, Sophmore</dd>
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<dd>The University of Tokyo, Arts and Sciences</dd>
<dt>Job</dt>
<dt>Job</dt>
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<dd>Modeling</dd>
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<dd>Team leader</dd>
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<p class ="comment">I want to be a doctor.</p>
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<p class ="comment">My dream is to make me robuster against stress caused by iGEM in synthetic biology!</p>
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<p class = "name">MASAKI ONO</p>
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<p class = "name" style="font-size:35px;">CRISTIAN DAVID PENA MARINEZ</p>
<img src = "https://static.igem.org/mediawiki/2014/f/f1/Chris_comb.png" class ="member-detail" />
<img src = "https://static.igem.org/mediawiki/2014/f/f1/Chris_comb.png" class ="member-detail" />
<dl class = "profile">
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<dt>Name</dt>
<dt>Name</dt>
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<dd>Masaki Ono</dd>
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<dd>Cristian David Pena Martinez</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
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<dd>The University of Tokyo, Literature, Sophmore</dd>
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<dd>Department of Regulatory Biology, Faculty of Science, Saitama University</dd>
<dt>Job</dt>
<dt>Job</dt>
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<dd>Modeling</dd>
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<dd>Design</dd>
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<p class ="comment">I want to be a doctor.</p>
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<p class ="comment">"The only concrete proof of the human existence is: poetry" - Luis Cardoza y Aragon</p>
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<dd>Masaki Ono</dd>
<dd>Masaki Ono</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
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<dd>The University of Tokyo, Literature, Sophmore</dd>
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<dd>The University of Tokyo, Arts and Sciences, Sophmore</dd>
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<dt>Job</dt>
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<p class ="comment">I want to be a doctor.</p>
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<p>iGEM UT-Tokyo</p>
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<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
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<p class = "name">MASAKI ONO</p>
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<dt>Name</dt>
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<dd>Masaki Ono</dd>
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<dt>Belong to</dt>
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<dd>The University of Tokyo, Literature, Sophmore</dd>
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<dt>Job</dt>
<dt>Job</dt>
<dd>Modeling</dd>
<dd>Modeling</dd>
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<div class = "member">
<div class = "member">
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<p class = "name">MASAKI ONO</p>
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<p class = "name">YUMENO KOGA</p>
<img src = "https://static.igem.org/mediawiki/2014/6/63/Koga_comb.png" class ="member-detail" />
<img src = "https://static.igem.org/mediawiki/2014/6/63/Koga_comb.png" class ="member-detail" />
<dl class = "profile">
<dl class = "profile">
<dt>Name</dt>
<dt>Name</dt>
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<dd>Masaki Ono</dd>
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<dd>Yumeno Koga</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
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<dd>The University of Tokyo, Literature, Sophmore</dd>
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<dd>Department of Lifescience & Medical Bioscience, School of Advanced Science and Engineering, Waseda University</dd>
<dt>Job</dt>
<dt>Job</dt>
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<dd>Modeling</dd>
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<dd>Presenter</dd>
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<p class ="comment">I want to be a doctor.</p>
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<p class ="comment">I'm a "wasejo".</p>
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<div class = "member">
<div class = "member">
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<p class = "name">MASAKI ONO</p>
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<p class = "name">KENTO NAKAMURA</p>
<img src = "https://static.igem.org/mediawiki/2014/8/87/Nakamura_comb.png" class ="member-detail" />
<img src = "https://static.igem.org/mediawiki/2014/8/87/Nakamura_comb.png" class ="member-detail" />
<dl class = "profile">
<dl class = "profile">
<dt>Name</dt>
<dt>Name</dt>
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<dd>Masaki Ono</dd>
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<dd>Kento Nakamura</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
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<dd>The University of Tokyo, Literature, Sophmore</dd>
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<dd>College of Arts and Sciences, The University of Tokyo</dd>
<dt>Job</dt>
<dt>Job</dt>
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<dd>Modeling</dd>
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<dd>Experimenter, Presenter</dd>
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<p class ="comment">I want to be a doctor.</p>
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<p class ="comment">"I think, therefore I am" by R. Descartes<br />"I multiply, therefore I am" by E.coli <br />"I hope so..." by Experimenter</p>
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<div class = "member">
<div class = "member">
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<p class = "name">MASAKI ONO</p>
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<p class = "name">SOH NAKASHIMA</p>
<img src = "https://static.igem.org/mediawiki/2014/7/74/Dolicas_comb.png" class ="member-detail" />
<img src = "https://static.igem.org/mediawiki/2014/7/74/Dolicas_comb.png" class ="member-detail" />
<dl class = "profile">
<dl class = "profile">
<dt>Name</dt>
<dt>Name</dt>
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<dd>Masaki Ono</dd>
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<dd>Soh Nakashima</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
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<dd>The University of Tokyo, Literature, Sophmore</dd>
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<dd>College of Arts and Sciences, The University of Tokyo</dd>
<dt>Job</dt>
<dt>Job</dt>
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<dd>Modeling</dd>
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<dd>Experimenter</dd>
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<p class ="comment">I want to be a doctor.</p>
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<p class ="comment">follow me on Twitter(<a href="https://twitter.com/dolicas_" target="_blank" >@dolicas_</a>)</p>
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<div class = "member">
<div class = "member">
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<p class = "name">MASAKI ONO</p>
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<p class = "name">MANABU NISHIURA</p>
<img src = "https://static.igem.org/mediawiki/2014/2/22/Nishiura_comb.png" class ="member-detail" />
<img src = "https://static.igem.org/mediawiki/2014/2/22/Nishiura_comb.png" class ="member-detail" />
<dl class = "profile">
<dl class = "profile">
<dt>Name</dt>
<dt>Name</dt>
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<dd>Masaki Ono</dd>
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<dd>Manabu Nishiura</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
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<dd>The University of Tokyo, Literature, Sophmore</dd>
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<dd>The University of Tokyo, Arts and Sciences</dd>
<dt>Job</dt>
<dt>Job</dt>
-
<dd>Modeling</dd>
+
<dd>Modeling,Presenter</dd>
</dl>
</dl>
-
<p class ="comment">I want to be a doctor.</p>
+
<p class ="comment"> I am a Feynman Diagram.</p>
</div>
</div>
</div>
</div>
Line 338: Line 271:
</div>
</div>
<div class = "member">
<div class = "member">
-
<p class = "name">MASAKI ONO</p>
+
<p class = "name">YOSHIKI OKESAKU</p>
<img src = "https://static.igem.org/mediawiki/2014/d/d0/Oke_comb.png" class ="member-detail" />
<img src = "https://static.igem.org/mediawiki/2014/d/d0/Oke_comb.png" class ="member-detail" />
<dl class = "profile">
<dl class = "profile">
<dt>Name</dt>
<dt>Name</dt>
-
<dd>Masaki Ono</dd>
+
<dd>Yoshiki Okesaku</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
-
<dd>The University of Tokyo, Literature, Sophmore</dd>
+
<dd> Department of Physics, Graduate School of Science, The University of Tokyo</dd>
<dt>Job</dt>
<dt>Job</dt>
-
<dd>Modeling</dd>
+
<dd>Design(DOKATA)</dd>
</dl>
</dl>
-
<p class ="comment">I want to be a doctor.</p>
+
<p class ="comment">I am majoring in the MDS theory. The MDS theory can describe how our universe had begun and will end.</p>
</div>
</div>
</div>
</div>
Line 362: Line 295:
</div>
</div>
<div class = "member">
<div class = "member">
-
<p class = "name">MASAKI ONO</p>
+
<p class = "name">HAJIME TAKEMURA</p>
<img src = "https://static.igem.org/mediawiki/2014/3/35/Takemura_comb.png" class ="member-detail" />
<img src = "https://static.igem.org/mediawiki/2014/3/35/Takemura_comb.png" class ="member-detail" />
<dl class = "profile">
<dl class = "profile">
<dt>Name</dt>
<dt>Name</dt>
-
<dd>Masaki Ono</dd>
+
<dd>Hajime Takemura</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
-
<dd>The University of Tokyo, Literature, Sophmore</dd>
+
<dd>The University of Tokyo, Junior,Faculty of Pharmaceutical  Science</dd>
<dt>Job</dt>
<dt>Job</dt>
-
<dd>Modeling</dd>
+
<dd>Experiment</dd>
</dl>
</dl>
-
<p class ="comment">I want to be a doctor.</p>
+
<p class ="comment">I want to make a new medicine.</p>
</div>
</div>
</div>
</div>
Line 386: Line 319:
</div>
</div>
<div class = "member">
<div class = "member">
-
<p class = "name">MASAKI ONO</p>
+
<p class = "name">KENTARO TARA</p>
<img src = "https://static.igem.org/mediawiki/2014/e/ef/Tara_comb.png" class ="member-detail" />
<img src = "https://static.igem.org/mediawiki/2014/e/ef/Tara_comb.png" class ="member-detail" />
<dl class = "profile">
<dl class = "profile">
<dt>Name</dt>
<dt>Name</dt>
-
<dd>Masaki Ono</dd>
+
<dd>Kentaro Tara</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
-
<dd>The University of Tokyo, Literature, Sophmore</dd>
+
<dd>Arts and Sciences, The University of Tokyo</dd>
<dt>Job</dt>
<dt>Job</dt>
-
<dd>Modeling</dd>
+
<dd>Experiment, Modeling</dd>
</dl>
</dl>
-
<p class ="comment">I want to be a doctor.</p>
+
<p class ="comment">Maps relax me.</p>
</div>
</div>
</div>
</div>
Line 410: Line 343:
</div>
</div>
<div class = "member">
<div class = "member">
-
<p class = "name">MASAKI ONO</p>
+
<p class = "name">YUEWEN DING</p>
<img src = "https://static.igem.org/mediawiki/2014/0/0e/Tei_comb.png" class ="member-detail" />
<img src = "https://static.igem.org/mediawiki/2014/0/0e/Tei_comb.png" class ="member-detail" />
<dl class = "profile">
<dl class = "profile">
<dt>Name</dt>
<dt>Name</dt>
-
<dd>Masaki Ono</dd>
+
<dd>Yuewen Ding</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
-
<dd>The University of Tokyo, Literature, Sophmore</dd>
+
<dd>Faculty of Pharmaceutical  Science, The university of Tokyo</dd>
<dt>Job</dt>
<dt>Job</dt>
-
<dd>Modeling</dd>
+
<dd>Public relation</dd>
</dl>
</dl>
-
<p class ="comment">I want to be a doctor.</p>
+
<p class ="comment">Eating is sweeter than Life.</p>
</div>
</div>
</div>
</div>
Line 434: Line 367:
</div>
</div>
<div class = "member">
<div class = "member">
-
<p class = "name">MASAKI ONO</p>
+
<p class = "name">YOSHIHIKO TOMOFUJI</p>
<img src = "https://static.igem.org/mediawiki/2014/2/21/Tomohuji_comb.png" class ="member-detail" />
<img src = "https://static.igem.org/mediawiki/2014/2/21/Tomohuji_comb.png" class ="member-detail" />
<dl class = "profile">
<dl class = "profile">
<dt>Name</dt>
<dt>Name</dt>
-
<dd>Masaki Ono</dd>
+
<dd>Yoshihiko Tomofuji</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
-
<dd>The University of Tokyo, Literature, Sophmore</dd>
+
<dd>The university of Tokyo, faculty of medicine</dd>
<dt>Job</dt>
<dt>Job</dt>
-
<dd>Modeling</dd>
+
<dd>E.coli</dd>
</dl>
</dl>
-
<p class ="comment">I want to be a doctor.</p>
+
<p class ="comment">I'm a E.coli.</p>
</div>
</div>
</div>
</div>
Line 458: Line 391:
</div>
</div>
<div class = "member">
<div class = "member">
-
<p class = "name">MASAKI ONO</p>
+
<p class = "name">KOHTA TOSHIMITSU</p>
<img src = "https://static.igem.org/mediawiki/2014/7/7c/Toshimitsu_comb.png" class ="member-detail" />
<img src = "https://static.igem.org/mediawiki/2014/7/7c/Toshimitsu_comb.png" class ="member-detail" />
<dl class = "profile">
<dl class = "profile">
<dt>Name</dt>
<dt>Name</dt>
-
<dd>Masaki Ono</dd>
+
<dd>Kohta Toshimitsu</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
-
<dd>The University of Tokyo, Literature, Sophmore</dd>
+
<dd>Faculty of Pharmacy,　Keio University</dd>
<dt>Job</dt>
<dt>Job</dt>
-
<dd>Modeling</dd>
+
<dd>Adviser</dd>
</dl>
</dl>
-
<p class ="comment">I want to be a doctor.</p>
+
<p class ="comment">I'm so sick of pipetting.</p>
</div>
</div>
</div>
</div>
Line 482: Line 415:
</div>
</div>
<div class = "member">
<div class = "member">
-
<p class = "name">MASAKI ONO</p>
+
<p class = "name">KEISUKE TSUKADA</p>
<img src = "https://static.igem.org/mediawiki/2014/0/0b/Tsukada_comb.png" class ="member-detail" />
<img src = "https://static.igem.org/mediawiki/2014/0/0b/Tsukada_comb.png" class ="member-detail" />
<dl class = "profile">
<dl class = "profile">
<dt>Name</dt>
<dt>Name</dt>
-
<dd>Masaki Ono</dd>
+
<dd>Keisuke Tsukada</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
-
<dd>The University of Tokyo, Literature, Sophmore</dd>
+
<dd>Department of Biophysics and Biochemistry , Faculty of Science, The University of Tokyo</dd>
<dt>Job</dt>
<dt>Job</dt>
-
<dd>Modeling</dd>
+
<dd>Experiment, Modeling</dd>
</dl>
</dl>
-
<p class ="comment">I want to be a doctor.</p>
+
<p class ="comment">itininnmaenokenkyuusyaninaretaraiina</p>
</div>
</div>
</div>
</div>
Line 506: Line 439:
</div>
</div>
<div class = "member">
<div class = "member">
-
<p class = "name">MASAKI ONO</p>
+
<p class = "name">YUTO YAMANAKA</p>
<img src = "https://static.igem.org/mediawiki/2014/2/26/Yamanaka_comb.png" class ="member-detail" />
<img src = "https://static.igem.org/mediawiki/2014/2/26/Yamanaka_comb.png" class ="member-detail" />
<dl class = "profile">
<dl class = "profile">
<dt>Name</dt>
<dt>Name</dt>
-
<dd>Masaki Ono</dd>
+
<dd>Yuto Yamanaka</dd>
<dt>Belong to</dt>
<dt>Belong to</dt>
-
<dd>The University of Tokyo, Literature, Sophmore</dd>
+
<dd>Department of Electrical Engineering and Bioscience, School of Advanced Science and Engineering, Waseda University</dd>
<dt>Job</dt>
<dt>Job</dt>
-
<dd>Modeling</dd>
+
<dd>Experimenter, Presenter</dd>
 +
</dl>
 +
<p class ="comment">I like Falcon tubes.</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class = "element-item">
 +
<div class = "thumbnail">
 +
<img src = "https://static.igem.org/mediawiki/2014/3/3e/Sumi.png" class ="member-thumbnail" />
 +
</div>
 +
<div class = "detail">
 +
<div class = "meta">
 +
<p>iGEM UT-Tokyo</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
 +
</div>
 +
<div class = "member">
 +
<p class = "name">SHUNSUKE SUMI</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/e/e8/Sumi_comb.png" class ="member-detail" />
 +
<dl class = "profile">
 +
<dt>Name</dt>
 +
<dd>Shunsuke Sumi</dd>
 +
<dt>Belong to</dt>
 +
<dd>Department of medicine, Faculty of medicine, The Jikei University School of Medicine</dd>
 +
<dt>Job</dt>
 +
<dd>tree</dd>
 +
</dl>
 +
<p class ="comment">a tree has no emotion.</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class = "element-item">
 +
<div class = "thumbnail">
 +
<img src = "https://static.igem.org/mediawiki/2014/3/34/Yoshikawa%281st%29.png" class ="member-thumbnail" />
 +
</div>
 +
<div class = "detail">
 +
<div class = "meta">
 +
<p>iGEM UT-Tokyo</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
 +
</div>
 +
<div class = "member">
 +
<p class = "name">NARUKI YOSHIKAWA</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/d/de/Yoshikawa%281st%29_comb.png" class ="member-detail" />
 +
<dl class = "profile">
 +
<dt>Name</dt>
 +
<dd>Naruki Yoshikawa</dd>
 +
<dt>Belong to</dt>
 +
<dd>College of Arts and Sciences, The University of Tokyo</dd>
 +
<dt>Job</dt>
 +
<dd>Experimenter</dd>
 +
</dl>
 +
<p class ="comment">Three billion devices run Java, but my devices don't.</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class = "element-item">
 +
<div class = "thumbnail">
 +
<img src = "https://static.igem.org/mediawiki/2014/9/95/Hiura.png" class ="member-thumbnail" />
 +
</div>
 +
<div class = "detail">
 +
<div class = "meta">
 +
<p>iGEM UT-Tokyo</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
 +
</div>
 +
<div class = "member">
 +
<p class = "name">NOBUHIRO HIURA</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/e/ea/Hiura_comb.png" class ="member-detail" />
 +
<dl class = "profile">
 +
<dt>Name</dt>
 +
<dd>Nobuhiro Hiura</dd>
 +
<dt>Belong to</dt>
 +
<dd>Department of Regulatory Biology, Faculty of Science, Saitama University</dd>
 +
<dt>Job</dt>
 +
<dd>Experimenter</dd>
 +
</dl>
 +
<p class ="comment">Let's go to Saitama, Japan!</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class = "element-item">
 +
<div class = "thumbnail">
 +
<img src = "https://static.igem.org/mediawiki/2014/8/8c/Tsuboi.png" class ="member-thumbnail" />
 +
</div>
 +
<div class = "detail">
 +
<div class = "meta">
 +
<p>iGEM UT-Tokyo</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
 +
</div>
 +
<div class = "member">
 +
<p class = "name">HIROKI TSUBOI</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/b/b6/Tsuboi_comb.png" class ="member-detail" />
 +
<dl class = "profile">
 +
<dt>Name</dt>
 +
<dd>Hiroki Tsuboi</dd>
 +
<dt>Belong to</dt>
 +
<dd>Waseda University, School of Fundamental Science and Engineering, Computer Science and Engineering</dd>
 +
<dt>Job</dt>
 +
<dd>Web</dd>
 +
</dl>
 +
<p class ="comment">After wiki freeze, I will make other Web Site</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class = "element-item">
 +
<div class = "thumbnail">
 +
<img src = "https://static.igem.org/mediawiki/2014/2/2b/Kobari.png" class ="member-thumbnail" />
 +
</div>
 +
<div class = "detail">
 +
<div class = "meta">
 +
<p>iGEM UT-Tokyo</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
 +
</div>
 +
<div class = "member">
 +
<p class = "name">SHIGETAKA KOBARI</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/f/fa/Kobari_comb.png" class ="member-detail" />
 +
<dl class = "profile">
 +
<dt>Name</dt>
 +
<dd>Shigetaka Kobari</dd>
 +
<dt>Belong to</dt>
 +
<dd>The university of Tokyo, faculty of medicine</dd>
 +
<dt>Job</dt>
 +
<dd>tree</dd>
 +
</dl>
 +
<p class ="comment">a tree has no emotion.</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class = "element-item">
 +
<div class = "thumbnail">
 +
<img src = "https://static.igem.org/mediawiki/2014/a/a1/Ishikawa.png" class ="member-thumbnail" />
 +
</div>
 +
<div class = "detail">
 +
<div class = "meta">
 +
<p>iGEM UT-Tokyo</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
 +
</div>
 +
<div class = "member">
 +
<p class = "name">MASATO ISHIKAWA</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/e/e9/Ishikawa_comb.png" class ="member-detail" />
 +
<dl class = "profile">
 +
<dt>Name</dt>
 +
<dd>Masato Ishikawa</dd>
 +
<dt>Belong to</dt>
 +
<dd>College of Arts and Sciences, The University of Tokyo</dd>
 +
<dt>Job</dt>
 +
<dd>Presenter</dd>
 +
</dl>
 +
<p class ="comment">I want to study pharmacy.</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class = "element-item">
 +
<div class = "thumbnail">
 +
<img src = "https://static.igem.org/mediawiki/2014/c/c6/Osawa.png" class ="member-thumbnail" />
 +
</div>
 +
<div class = "detail">
 +
<div class = "meta">
 +
<p>iGEM UT-Tokyo</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
 +
</div>
 +
<div class = "member">
 +
<p class = "name">MASAYUKI OSAWA</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/1/12/Osawa_comb.png" class ="member-detail" />
 +
<dl class = "profile">
 +
<dt>Name</dt>
 +
<dd>Masayuki Osawa</dd>
 +
<dt>Belong to</dt>
 +
<dd>Department of medicine, Faculty of medicine, The Jikei University School of Medicine</dd>
 +
<dt>Job</dt>
 +
<dd>Woodkeeper</dd>
 +
</dl>
 +
<p class ="comment">I take care of crazy tree.</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class = "element-item">
 +
<div class = "thumbnail">
 +
<img src = "https://static.igem.org/mediawiki/2014/5/53/Tube.png" class ="member-thumbnail" />
 +
</div>
 +
<div class = "detail">
 +
<div class = "meta">
 +
<p>iGEM UT-Tokyo</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
 +
</div>
 +
<div class = "member">
 +
<p class = "name">TAKEHUMI YOSHIKAWA</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/b/b1/Tube_comb.png" class ="member-detail" />
 +
<dl class = "profile">
 +
<dt>Name</dt>
 +
<dd>Takehumi Yoshikawa</dd>
 +
<dt>Belong to</dt>
 +
<dd>Department of Bioinformatics and Systems Biology, Faculty of Science, The University of Tokyo</dd>
 +
<dt>Job</dt>
 +
<dd>Experiment Leader</dd>
 +
</dl>
 +
<p class ="comment">I want to be Spe1.</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class = "element-item">
 +
<div class = "thumbnail">
 +
<img src = "https://static.igem.org/mediawiki/2014/d/d4/Ito.png" class ="member-thumbnail" />
 +
</div>
 +
<div class = "detail">
 +
<div class = "meta">
 +
<p>iGEM UT-Tokyo</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
 +
</div>
 +
<div class = "member">
 +
<p class = "name">ATSUKI ITO</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/1/1d/Ito_comb.png" class ="member-detail" />
 +
<dl class = "profile">
 +
<dt>Name</dt>
 +
<dd>Atsuki Ito</dd>
 +
<dt>Belong to</dt>
 +
<dd>Department of Bioscience, Faculty of Engineering,Tokyo University of Science</dd>
 +
<dt>Job</dt>
 +
<dd>Experimenter</dd>
 +
</dl>
 +
<p class ="comment">kimuwaipu,azinai...</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class = "element-item">
 +
<div class = "thumbnail">
 +
<img src = "https://static.igem.org/mediawiki/2014/3/3e/Prof_wakamoto.png" class ="member-thumbnail" />
 +
</div>
 +
<div class = "detail">
 +
<div class = "meta">
 +
<p>iGEM UT-Tokyo</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
 +
</div>
 +
<div class = "member">
 +
<p class = "name">PROF.WAKAMOTO</p>
 +
<img src = "https://static.igem.org/mediawiki/2014/f/fb/Prof_wakamoto_comb.png" class ="member-detail" />
 +
<dl class = "profile">
 +
<dt>Name</dt>
 +
<dd>Yuichi Wakamoto</dd>
 +
<dt>Belong to</dt>
 +
<dd>Center for Complex Systems Biology The University of Tokyo, Dapartment of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo</dd>
 +
<dt>Job</dt>
 +
<dd>Instructor</dd>
</dl>
</dl>
-
<p class ="comment">I want to be a doctor.</p>
 
</div>
</div>
</div>
</div>
Line 522: Line 694:
</div>
</div>
</div>
</div>
-
<!-- Teamのトップここまで -->
 
<!-- 上へ戻るボタン -->
<!-- 上へ戻るボタン -->
<div id="bttop" style="display:none;">
<div id="bttop" style="display:none;">
<a href="Javascript:bttop()"><img src="https://static.igem.org/mediawiki/2014/0/06/Topbutton.png" /></a>
<a href="Javascript:bttop()"><img src="https://static.igem.org/mediawiki/2014/0/06/Topbutton.png" /></a>
</div>
</div>
-
<!-- 上へ戻るボタン -->
 
</div>
</div>
</div>
</div>

Latest revision as of 03:02, 18 October 2014

Retrieved from "http://2014.igem.org/Team:UT-Tokyo/CTCD/Content"