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Team:Tsinghua-A
From 2014.igem.org
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<h3>Transactivator-Like Effectors (TALEs) are a technology that once revolutionized the way researchers manipulate DNA with exceptional site specificity.Though it works well in eukaryotic organisms, it is not available in prokaryotic organisms. We come up with the idea that the long repeating sequence of the TALEs coding sequence leads to it. In our project, we attempt to construct a marvelous TALEs system which can efficiently work in prokaryotic organisms. By optimizing the TALEs coding sequence with modeling methods and testing it by Golden Gate Assembly and Report System, we hope we can have a handle tool for gene editing in prokaryotic organisms. | <h3>Transactivator-Like Effectors (TALEs) are a technology that once revolutionized the way researchers manipulate DNA with exceptional site specificity.Though it works well in eukaryotic organisms, it is not available in prokaryotic organisms. We come up with the idea that the long repeating sequence of the TALEs coding sequence leads to it. In our project, we attempt to construct a marvelous TALEs system which can efficiently work in prokaryotic organisms. By optimizing the TALEs coding sequence with modeling methods and testing it by Golden Gate Assembly and Report System, we hope we can have a handle tool for gene editing in prokaryotic organisms. | ||
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<p>Background, hypothesis and workflow of designer TALE. </p> | <p>Background, hypothesis and workflow of designer TALE. </p> | ||
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<h2>Modeling</h2> | <h2>Modeling</h2> | ||
<p>Optimization of TALE sequence and simulation of the gene circuit. </p> | <p>Optimization of TALE sequence and simulation of the gene circuit. </p> | ||
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<h2>Human Practice</h2> | <h2>Human Practice</h2> | ||
<p>Lectures, seminar, questionnaires and raising ethic problems. </p> | <p>Lectures, seminar, questionnaires and raising ethic problems. </p> | ||
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<h2>Team</h2> | <h2>Team</h2> | ||
<p>Department of Automatics, Life Science and Medical School. </p> | <p>Department of Automatics, Life Science and Medical School. </p> | ||
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Latest revision as of 03:01, 18 October 2014
Introduction
Transactivator-Like Effectors (TALEs) are a technology that once revolutionized the way researchers manipulate DNA with exceptional site specificity.Though it works well in eukaryotic organisms, it is not available in prokaryotic organisms. We come up with the idea that the long repeating sequence of the TALEs coding sequence leads to it. In our project, we attempt to construct a marvelous TALEs system which can efficiently work in prokaryotic organisms. By optimizing the TALEs coding sequence with modeling methods and testing it by Golden Gate Assembly and Report System, we hope we can have a handle tool for gene editing in prokaryotic organisms.
Team
Acknowledgements
