Team:Tsinghua-A

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             <h1>Welcome to Tsinghua-A Team Wiki</h1>
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             <h2> To TALE a new world.  <a href="https://2014.igem.org/Team:Tsinghua-A/Project"><i class="icon-chevron-right"></i> See Project</a></h2>
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             <h1>Welcome to Tsinghua-A Team Wiki</h1>
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            <h2> Coding to reveal the code.   <a href="https://2014.igem.org/Team:Tsinghua-A/Modeling"><i class="icon-chevron-right"></i> See Modeling</a> </h2>
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             <h1>Welcome to Tsinghua-A Team Wiki</h1>
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            <h2> Practice humanity with technology. <a href="https://2014.igem.org/Team:Tsinghua-A/HumanPractice"><i class="icon-chevron-right"></i> See Human Practice</a> </h2>
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        <h2> Introduction </h2>
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          <h3>Transactivator-Like Effectors (TALEs) are a technology that once revolutionized the way researchers manipulate DNA with exceptional site specificity.Though it works well in eukaryotic organisms, it is not available in prokaryotic organisms. We come up with the idea that the long repeating sequence of the TALEs coding sequence leads to it. In our project, we attempt to construct a marvelous TALEs system which can efficiently work in prokaryotic organisms. By optimizing the TALEs coding sequence with modeling methods and testing it by Golden Gate Assembly and Report System, we hope we can have a handle tool for gene editing in prokaryotic organisms.
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          <a href="https://2014.igem.org/Team:Tsinghua-A/Project">
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            <h2>Project</h2>
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            <p>Background, hypothesis and workflow of designer TALE. </p>
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            <h2>Modeling</h2>
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            <p>Optimization of TALE sequence and simulation of the gene circuit. </p>
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            <h2>Human Practice</h2>
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            <p>Lectures, seminar, questionnaires and raising ethic problems.  </p>
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            <h2>Team</h2>
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            <p>Department of Automatics, Life Science and Medical School. </p>
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font-weight:bold;
 
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font-family:Verdana, Arial, Helvetica, sans-serif;
 
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}
 
-
 
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#topnav ul, #topnav li{
 
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float:left;
 
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list-style:none;
 
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margin:0;
 
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padding:0;
 
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}
 
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#topnav li a:link, #topnav li a:visited, #topnav li a:hover{
 
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display:block;
 
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margin:0;
 
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padding:15px 20px;
 
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color:#999999;
 
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background-color:#333333;
 
-
}
 
-
 
-
#topnav ul ul li a:link, #topnav ul ul li a:visited{
 
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border:none;
 
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}
 
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#topnav li.last a{
 
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margin-right:0;
 
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}
 
-
 
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#topnav li a:hover, #topnav ul li.active a{
 
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color:#FFFFFF;
 
-
background-color:#B2C629;
 
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}
 
-
 
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#topnav li li a:link, #topnav li li a:visited{
 
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width:150px;
 
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float:none;
 
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margin:0;
 
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padding:7px 10px;
 
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font-size:12px;
 
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font-weight:normal;
 
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}
 
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#topnav li ul{
 
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z-index:9999;
 
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position:absolute;
 
-
left:-999em;
 
-
height:auto;
 
-
width:170px;
 
-
border-left:1px solid #CCCCCC;
 
-
border-bottom:1px solid #CCCCCC;
 
-
}
 
-
 
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#topnav li ul a{width:140px;}
 
-
 
-
#topnav li ul ul{margin:-32px 0 0 0;}
 
-
 
-
#topnav li:hover ul ul{left:-999em;}
 
-
 
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#topnav li:hover ul, #topnav li li:hover ul{left:auto;}
 
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-
#topnav li:hover{position:static;}
 
-
 
-
#topnav li.last a{margin-right:0;}
 
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-
/* ----------------------------------------------Column Navigation-------------------------------------*/
 
-
 
-
#column .subnav{display:block; width:250px; padding:25px; background-color:#F9F9F9; margin-bottom:30px;}
 
-
 
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#column .subnav h2{
 
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margin:0 0 20px 0;
 
-
padding:0 0 14px 0;
 
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font-size:20px;
 
-
font-weight:normal;
 
-
font-family:Georgia, "Times New Roman", Times, serif;
 
-
color:#666666;
 
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background-color:#F9F9F9;
 
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line-height:normal;
 
-
border-bottom:1px dotted #666666;
 
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}
 
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#column .subnav ul{
 
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margin:0;
 
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padding:0;
 
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list-style:none;
 
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}
 
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#column .subnav li{
 
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margin:0 0 3px 0;
 
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padding:0;
 
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}
 
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-
#column .subnav ul ul, #column .subnav ul ul ul, #column .subnav ul ul ul ul, #column .subnav ul ul ul ul ul{border-top:none; padding-top:0;}
 
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#column .subnav a{
 
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display:block;
 
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margin:0;
 
-
padding:5px 10px 5px 20px;
 
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color:#777777;
 
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background:url("../images/green_file.gif") no-repeat 10px center #F9F9F9;
 
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text-decoration:none;
 
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border-bottom:1px dotted #666666;
 
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}
 
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#column .subnav a:hover{color:#B2C629; background-color:#F9F9F9;}
 
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-
#column .subnav ul ul a, #column .subnav ul ul ul a, #column .subnav ul ul ul ul a, #column .subnav ul ul ul ul ul a{background:url("../images/black_file.gif") no-repeat #F9F9F9;}
 
-
#column .subnav ul ul a{padding-left:40px; background-position:30px center;}
 
-
#column .subnav ul ul ul a{padding-left:50px; background-position:40px center;}
 
-
#column .subnav ul ul ul ul a{padding-left:60px; background-position:50px center;}
 
-
#column .subnav ul ul ul ul ul a{padding-left:70px; background-position:60px center;}
 
-
</style>
 
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-
<style type="text/css">
 
-
/*
 
-
Template Name: PlusBusiness
 
-
File: Tables CSS
 
-
Author: OS Templates
 
-
Author URI: #
 
-
Licence: <a href="#">Website Template Licence</a>
 
-
*/
 
-
 
-
table{
 
-
width:100%;
 
-
border-collapse:collapse;
 
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table-layout:auto;
 
-
vertical-align:top;
 
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margin-bottom:15px;
 
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border:1px solid #CCCCCC;
 
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}
 
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-
table thead th{
 
-
color:#FFFFFF;
 
-
background-color:#666666;
 
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border:1px solid #CCCCCC;
 
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border-collapse:collapse;
 
-
text-align:center;
 
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table-layout:auto;
 
-
vertical-align:middle;
 
-
}
 
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-
table tbody td{
 
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vertical-align:top;
 
-
border-collapse:collapse;
 
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border-left:1px solid #CCCCCC;
 
-
border-right:1px solid #CCCCCC;
 
-
}
 
-
 
-
table thead th, table tbody td{
 
-
padding:5px;
 
-
border-collapse:collapse;
 
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}
 
-
 
-
table tbody tr.light{
 
-
color:#666666;
 
-
background-color:#F7F7F7;
 
-
}
 
-
 
-
table tbody tr.dark{
 
-
color:#666666;
 
-
background-color:#E8E8E8;
 
-
}
 
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</style>
 
-
 
-
<title>PlusBusiness</title>
 
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 
-
<meta http-equiv="imagetoolbar" content="no" />
 
-
<link rel="stylesheet" href="styles/layout.css" type="text/css" />
 
-
<script type="text/javascript" src="scripts/jquery-1.4.1.min.js">
 
-
/*!
 
-
* jQuery JavaScript Library v1.4.1
 
-
* http://jquery.com/
 
-
*
 
-
* Copyright 2010, John Resig
 
-
* Dual licensed under the MIT or GPL Version 2 licenses.
 
-
* http://jquery.org/license
 
-
*
 
-
* Includes Sizzle.js
 
-
* http://sizzlejs.com/
 
-
* Copyright 2010, The Dojo Foundation
 
-
* Released under the MIT, BSD, and GPL Licenses.
 
-
*
 
-
* Date: Mon Jan 25 19:43:33 2010 -0500
 
-
*/
 
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* jCarousel - Riding carousels with jQuery
 
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*  http://sorgalla.com/jcarousel/
 
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*
 
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* Dual licensed under the MIT (MIT-LICENSE.txt)
 
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* and GPL (GPL-LICENSE.txt) licenses.
 
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*
 
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*  http://jquery.com
 
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*
 
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Latest revision as of 03:01, 18 October 2014

Title

Introduction

Transactivator-Like Effectors (TALEs) are a technology that once revolutionized the way researchers manipulate DNA with exceptional site specificity.Though it works well in eukaryotic organisms, it is not available in prokaryotic organisms. We come up with the idea that the long repeating sequence of the TALEs coding sequence leads to it. In our project, we attempt to construct a marvelous TALEs system which can efficiently work in prokaryotic organisms. By optimizing the TALEs coding sequence with modeling methods and testing it by Golden Gate Assembly and Report System, we hope we can have a handle tool for gene editing in prokaryotic organisms.

Project

Background, hypothesis and workflow of designer TALE.

Modeling

Optimization of TALE sequence and simulation of the gene circuit.

Human Practice

Lectures, seminar, questionnaires and raising ethic problems.

Team

Department of Automatics, Life Science and Medical School.

Team

Title

Acknowledgements

Title

Retrieved from "http://2014.igem.org/Team:Tsinghua-A"