Team:Penn/Magnetism

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<title>University of Pennsylvania iGEM</title>
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<a href="https://2014.igem.org/Team:Penn"><li>Home</li></a>
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      <a href="https://2014.igem.org/Team:Penn/Overview"> <li>Overview</li> </a>
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    <a href="https://2014.igem.org/Team:Penn/Magnetism"> <li>Magnetism</li> </a>
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<div style = "text-align: center; font-size: 24px;">Magnetic Properties of AMB-1</div></br>
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<div style = "text-align: center;"><img width="300px" src="https://static.igem.org/mediawiki/2014/c/c5/Magnetic-props-header.png"></div><br>
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<h3>Relationship Between OD600 T2 for AMB-1</h3>
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<p>We attempted to explore the magnetic properties of AMB-1 as further understanding would prove AMB-1’s value as a chassis in bioremediation and other fields. We attempted to quantify the magnetic strength of AMB-1 (T2 time) and relate it to the cell concentration (OD600). To do so, we used a magnetic NMR machine (the minispec mq60, Bruker) to measure the T2 decay time of cell samples. A longer T2 time indicated fewer magnetic particles.
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<p style = "text-align: left; text-indent:0px"> AMB-1 is most useful for synthetic biology because of its rare capacity to align with magnetic fields.<sup>[9]</sup> We attempted to explore the magnetic properties of AMB-1 as further understanding would prove AMB-1’s value as a chassis in bioremediation and other fields. </p>
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<p>AMB-1 was grown under aerobic conditions to saturation. The culture was then serially diluted into ½, ¼ and 1/8 of the initial concentration. The OD600 and T2 time of all dilution samples was measured and then recorded in the table below (Table 4.1). One 1 mL of the cell sample was used for each measurement.  MSGM media that does not contain bacteria was used as a blank for this experiment (the OD600 for the blank is 0).
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<h3 style= "text-align: left">Relationship Between OD<sub>600</sub> and T<sub>2</sub> for AMB-1</h3>
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<p style = "text-align: left; text-indent:0px">We hoped to quantify the magnetic strength of AMB-1 using spin-spin relaxation time (T<sub>2</sub> time) and relate it to the cell concentration (OD<sub>600</sub>). To do so, we used a magnetic NMR machine (the minispec mq60, Bruker) to measure the T<sub>2</sub> decay time of cell samples (Fig. 1). A longer T2 time indicated fewer magnetic particles, and therefore weaker magnetic properties.
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<div id = "figureBox" style = "margin-left: auto; margin-right:auto; width: 300px; text-align:center;"> Table 4.1- Relationship between OD600 and T2 for AMB-1</br><img style = "width: 300px;" src = "Figure 5.png">
 
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<h3 style= "text-align: left; font-size: 18px;">Figure 1:</h3>
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<div id = "figureBox" style = "margin-left: auto; margin-right:auto; width: 700px; text-align:center;"> <img style = "width: 700px;" src = "https://static.igem.org/mediawiki/2014/d/dc/Magnetism_and_AMB-1.png"></div>
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<h3 style= "text-align: left; font-size: 18px;">Results:</h3>
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<p style = "text-align: left; text-indent:0px">Since the relationship between the inverse of T<sub>2</sub> and OD was linear, this data supports that each cell has roughly the same number of magnetosomes, and that a greater number of these magnetosomes can be correlated with stronger magnetic properties. The cell concentration and magnetic strength showed a linear relationship <b>(1/T<sub>2</sub>) =(0.002x + 0.0016)</b> with an R<sup>2</sup> = 0.704.
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<h3>Achievement:</h3>
 
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<p>The cell concentration and magnetic strength showed a linear relationship (1T2 =0.0032x + 0.0015) with an R^2 value that was very close to one.
 
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<a href="https://2014.igem.org/Team:Penn/Microbio"><img width="80px" src="https://static.igem.org/mediawiki/2014/thumb/archive/c/ca/20141018025849%21Syn-bio-amb-1-flow-arrow.png/120px-Syn-bio-amb-1-flow-arrow.png">;
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Latest revision as of 03:01, 18 October 2014

University of Pennsylvania iGEM

AMB-1 is most useful for synthetic biology because of its rare capacity to align with magnetic fields.[9] We attempted to explore the magnetic properties of AMB-1 as further understanding would prove AMB-1’s value as a chassis in bioremediation and other fields.

Relationship Between OD600 and T2 for AMB-1

We hoped to quantify the magnetic strength of AMB-1 using spin-spin relaxation time (T2 time) and relate it to the cell concentration (OD600). To do so, we used a magnetic NMR machine (the minispec mq60, Bruker) to measure the T2 decay time of cell samples (Fig. 1). A longer T2 time indicated fewer magnetic particles, and therefore weaker magnetic properties.

Figure 1:

Results:

Since the relationship between the inverse of T2 and OD was linear, this data supports that each cell has roughly the same number of magnetosomes, and that a greater number of these magnetosomes can be correlated with stronger magnetic properties. The cell concentration and magnetic strength showed a linear relationship (1/T2) =(0.002x + 0.0016) with an R2 = 0.704.

The following video of AMB-1 was taken under 1000X magnification.


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