"
Team:Penn/Magnetism
From 2014.igem.org
Ameyvrudhula (Talk | contribs) |
|||
| Line 47: | Line 47: | ||
</div> | </div> | ||
<div style="position:absolute;right:5px;bottom:50px"> | <div style="position:absolute;right:5px;bottom:50px"> | ||
| - | <a href="https://2014.igem.org/Team:Penn/Microbio"><img width="80px" src="https://static.igem.org/mediawiki/2014/c/ca/Syn-bio-amb-1-flow-arrow.png">; | + | <a href="https://2014.igem.org/Team:Penn/Microbio"><img width="80px" src="https://static.igem.org/mediawiki/2014/thumb/archive/c/ca/20141018025849%21Syn-bio-amb-1-flow-arrow.png/120px-Syn-bio-amb-1-flow-arrow.png">; |
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> | ||
Revision as of 03:00, 18 October 2014
AMB-1 is most useful for synthetic biology because of its rare capacity to align with magnetic fields. We attempted to explore the magnetic properties of AMB-1 as further understanding would prove AMB-1’s value as a chassis in bioremediation and other fields.
Relationship Between OD600 and T2 for AMB-1
We hoped to quantify the magnetic strength of AMB-1 using spin-spin relaxation time (T2 time) and relate it to the cell concentration (OD600). To do so, we used a magnetic NMR machine (the minispec mq60, Bruker) to measure the T2 decay time of cell samples (Fig. 1). A longer T2 time indicated fewer magnetic particles, and therefore weaker magnetic properties.
Figure 1:
Results:
Since the relationship between the inverse of T2 and OD was linear, this data supports that each cell has roughly the same number of magnetosomes, and that a greater number of these magnetosomes can be correlated with stronger magnetic properties. The cell concentration and magnetic strength showed a linear relationship (1/T2) =(0.002x + 0.0016) with an R2 = 0.704.
The following video of AMB-1 was taken under 1000X magnification.
;
