Team:Penn/Overview

From 2014.igem.org

(Difference between revisions)
(edit the nav bar)
 
(26 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Team:Penn/CSS}}
<html>
<html>
-
<head>
 
-
<style>
 
-
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
 
-
#content { /*-- changes default wiki settings --*/
+
<head>
-
width: 100%;  
+
-
height: 100%;
+
<title>University of Pennsylvania iGEM</title>
-
border: none;
+
<link href='http://fonts.googleapis.com/css?family=Open+Sans' rel='stylesheet' type='text/css'>
-
background-color: transparent;
+
<style>
-
margin: 0px;
+
#menuicon2 {
-
padding: 0px;
+
width: 180px;
 +
        height: 180px;
 +
        margin-left: auto;
 +
margin-right: auto;
 +
vertical-align: center;
 +
margin: 25px;
 +
box-shadow: 0px 0px 10px #000000;
}
}
 +
</style>
 +
</head>
-
#menubar {
+
<body>
-
    width: auto;}
+
 +
<div id="redbox">
 +
<div style = "text-align: center;"><img width="200px" src="https://static.igem.org/mediawiki/2014/8/86/Syn_bio_amb-1_flow.jpg"></div><br>
 +
<div id="textbox">
 +
<p>Synthetic biologists engineer organisms for impactful applications ranging from bacterial biosensors for disease diagnostics to microbial strains capable of cleaning up radiation. Largely, these projects utilize the reliable “powerhouse” host cell of synthetic biology: E.Coli. This strain serves as a valuable chassis for most projects as it is well-characterized and easy to transform with engineered DNA parts. If researchers could easily engineer rarer strains of bacteria and take advantage of their biological diversity, the field would open up to new applications that leverage the unique attributes of these unconventional chassis.
 +
</p>
-
#contentSub, #search-controls, .firstHeading, #footer-box, #catlinks, #p-logo {
+
<p>We characterized a fascinating and underused organism: Magnetospirillum magneticum (AMB-1), a bacterium that aligns with magnetic fields. AMB-1 had previously been incorporated in very few publications. By developing, testing, and optimizing protocols for its growth and transformation, and then making them easily accessible in a convenient Strain Spec Sheet, we hope to establish AMB-1 as an easily engineered organism. We also tested and troubleshot the few genetic parts used in AMB-1 engineering, and then designed a new, BioBrick-compatible vector with an AMB-1 specific origin of replication, promoter, and multiple-cloning site. We are currently characterizing this vector, pMAGMA3.
-
    display:none;}
+
</p>
-
#top-section {
+
-
    border: none;
+
-
    height: 0px;}
+
-
#top-section-bar {
+
-
border: none;
+
-
    height: 0px;
+
-
}
+
-
/*Remove "teams" from the menubar*/
+
-
#menubar > ul > li:last-child {
+
-
    display: none;}
+
-
/*Transparent the log in/out button*/
+
-
#menubar {
+
-
background-color: transparent;
+
-
}
+
-
#menubar li a {
+
-
    color: transparent;
+
-
    background-color: transparent;}
+
-
#menubar li{
+
-
    background-color: transparent;}
+
-
#menubar:hover li a {
+
-
    color: #777;}
+
-
</style>
+
<p>In the future, AMB-1 has high potential for use in novel synthetic biology applications because of its capacity to align with magnetic fields.  We are especially interested in using AMB-1 for bioremediation applications, such as cleaning pollutants from water. Many engineered microbes can absorb pollutants, but if AMB-1 were used instead of E.Coli, it could be subsequently removed from the water with a magnet – effectively removing both the pollutant and the engineered microbe. To help prove this concept is feasible, we ran experiments to test that AMB-1 can survive in water polluted with the heavy metal, cadmium.
-
<link href="https://98c43ce680a0761e7c3ad3c1d7ae34f6de6db886.googledrive.com/host/0B9QyOqpKYA2gdGV2aGFRMWh4aXM/project_overview.css" rel="stylesheet" type="text/css">
+
</p>
 +
</div>
 +
<br>
 +
<table>
 +
<tr>
 +
<td ><a href="https://2014.igem.org/Team:Penn/Magnetism"><img
 +
id = "menuicon2" src="https://static.igem.org/mediawiki/2014/8/86/Penn_2014_wiki_4.png"></a></td>
 +
<td><a href="https://2014.igem.org/Team:Penn/Microbio"><img id = "menuicon2" src="https://static.igem.org/mediawiki/2014/d/df/Magnetic_bacteria_graphic.png"></a></td>
 +
<td ><a href="https://2014.igem.org/Team:Penn/Synbio"><img
 +
id = "menuicon2" src="https://static.igem.org/mediawiki/2014/6/6e/Penn_2014_wiki_2.png"></a></td>
 +
<td><a href="https://2014.igem.org/Team:Penn/CdTolerance"><img
 +
id = "menuicon2" src="https://static.igem.org/mediawiki/2014/9/9e/Penn_2014_wiki_3.png"></a></td>
-
</head>
 
-
<!-- beginning of your page -->
+
</tr><tr>
-
<body>
+
<td style = "text-align: center;"><a  
-
<!-- navigation bar -->
+
href="https://2014.igem.org/Team:Penn/Magnetism">Magnetism of AMB-1</a></td>
-
<nav>
+
<td style = "text-align: center;"><a href="https://2014.igem.org/Team:Penn/Microbio">Microbiology in AMB-1</a></td>
-
<div id="school_title">
+
<td style = "text-align: center;"><a href="https://2014.igem.org/Team:Penn/Microbio/Synbio">Synthetic Biology in AMB-1</a></td>
-
<p><a href="http://www.upenn.edu">University of Pennsylvania</a> <span>iGEM 2014</span></p>
+
<td style = "text-align: center;"><a href="https://2014.igem.org/Team:Penn/CdTolerance">Cadmium Tolerance in E. Coli vs. AMB-1</a></td>
-
</div>
+
-
<ul>
+
-
<li><a href="https://2014.igem.org/Team:Penn"><span class="menu_item">Home</span></a></li>
+
-
    <li>|</li>
+
-
<li><a href="https://2014.igem.org/Team:Penn/Overview" ><span class="menu_item">Project</span></a>
+
-
<ul class="sub_menu">
+
-
<li><a href="https://2014.igem.org/Team:Penn/Overview">Overview</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Penn/Motivation">Motivation</a></li>
+
-
</ul>
+
-
</li>
+
-
    <li>|</li>
+
-
<li><a href="https://2014.igem.org/Team:Penn/Protocol"><span class="menu_item">Wet lab</span></a>
+
-
<ul class="sub_menu">
+
-
                        <li><a href="https://2014.igem.org/Team:Penn/Protocol">Protocol</a></li>
+
-
                        <li><a href="https://2014.igem.org/Team:Penn/Safety">Safety</a></li>
+
-
                        <li><a href="https://2014.igem.org/Team:Penn/Notebook">Notebook</a></li>
+
-
</ul>
+
-
</li>
+
-
  <li>|</li>
+
-
<li><a href="https://2014.igem.org/Team:Penn/Outreach"><span class="menu_item">Human Practices</span></a></li>
+
-
              <ul class="sub_menu">
+
-
                        <li><a href="https://2014.igem.org/Team:Penn/Outreach">Outreach</a></li>
+
-
                        <li><a href="">Biomeme</a></li>
+
-
</ul>
+
-
  <li>|</li>
+
-
<li><a href="https://2014.igem.org/Team:Penn/Team"><span class="menu_item">About us</span></a>
+
-
    <ul class="sub_menu">
+
-
<li><a href="https://2014.igem.org/Team:Penn/Team">Team</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Penn/Sponsors">Sponsors</a></li>
+
-
</ul>
+
-
    </li>
+
-
   
+
-
    <div id="igem_logo"><a href="https://igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="50px"></a>
+
-
</div>
+
-
</ul>
+
-
</nav>
+
-
<!-- end of navigation bar -->
+
-
<!--start of content block -->
+
</tr><tr>
-
<div id="wrapper">
+
-
</div>
+
-
<!-- end of content block -->
+
-
<!-- start of footer-->
+
-
<div id="footer">
+
-
<object id="follow_me"width='101' height='48'><embed src='http://www.socialbuttonmaker.com/Swfs/45da3933-8326-4170-89c0-10dea0659e98/button.swf' type='application/x-shockwave-flash' width='101' height='48' loop='false' wmode='transparent'><noscript><a href='http://soccerisland.info/'>island soccer news</a></noscript></embed><img src='' width=0 height=0 id='flashbutton'/><script type='text/javascript' src='http://www.socialbuttonmaker.com/Scripts/FlashButton.js'></script></object>
+
</table>
-
<p>Penn iGEM  &copy; 2014</p>
+
<div style="position:absolute;right:5px;bottom:50px">
 +
<a href="https://2014.igem.org/Team:Penn/Magnetism"><img width="80px" src="https://static.igem.org/mediawiki/2014/thumb/archive/c/ca/20141018025849%21Syn-bio-amb-1-flow-arrow.png/120px-Syn-bio-amb-1-flow-arrow.png">;
</div>
</div>
-
<!-- end of footer -->
+
</body>
-
</body>
+
</html>
</html>

Latest revision as of 02:59, 18 October 2014

University of Pennsylvania iGEM

Synthetic biologists engineer organisms for impactful applications ranging from bacterial biosensors for disease diagnostics to microbial strains capable of cleaning up radiation. Largely, these projects utilize the reliable “powerhouse” host cell of synthetic biology: E.Coli. This strain serves as a valuable chassis for most projects as it is well-characterized and easy to transform with engineered DNA parts. If researchers could easily engineer rarer strains of bacteria and take advantage of their biological diversity, the field would open up to new applications that leverage the unique attributes of these unconventional chassis.

We characterized a fascinating and underused organism: Magnetospirillum magneticum (AMB-1), a bacterium that aligns with magnetic fields. AMB-1 had previously been incorporated in very few publications. By developing, testing, and optimizing protocols for its growth and transformation, and then making them easily accessible in a convenient Strain Spec Sheet, we hope to establish AMB-1 as an easily engineered organism. We also tested and troubleshot the few genetic parts used in AMB-1 engineering, and then designed a new, BioBrick-compatible vector with an AMB-1 specific origin of replication, promoter, and multiple-cloning site. We are currently characterizing this vector, pMAGMA3.

In the future, AMB-1 has high potential for use in novel synthetic biology applications because of its capacity to align with magnetic fields. We are especially interested in using AMB-1 for bioremediation applications, such as cleaning pollutants from water. Many engineered microbes can absorb pollutants, but if AMB-1 were used instead of E.Coli, it could be subsequently removed from the water with a magnet – effectively removing both the pollutant and the engineered microbe. To help prove this concept is feasible, we ran experiments to test that AMB-1 can survive in water polluted with the heavy metal, cadmium.


Magnetism of AMB-1 Microbiology in AMB-1 Synthetic Biology in AMB-1 Cadmium Tolerance in E. Coli vs. AMB-1
;

Retrieved from "http://2014.igem.org/Team:Penn/Overview"