Team:UESTC-China/Futurework

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<a href="https://2014.igem.org/Team:UESTC-China/Futurework"><li>Future work</li></a>
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<a href="https://2014.igem.org/Team:UESTC-China/Modeling3"><li>AtAHA2</a>
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<a href="https://2014.igem.org/Team:UESTC-China/Safety"><li>Safety</li></a>
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<h1 style="color:#1b1b1b; position:relative; left:0px; padding:15 5px; font-size:40px; font-family: calibri, arial, helvetica, sans-serif; font-weight: bold;font-style: Italic; text-align:center; width:1140px;">Future work</h1>
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  <h1 class="SectionTitles" style="width:1100px; ">Different parts affect the efficiency of degrading formaldehyde
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</h1>
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We have constructed individual vectors with and without transit peptides, and vectors containing different part combinations. We hope to know the effects of transit peptides and different genes on the efficiency of degrading formaldehyde. And this work is ongoing.
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  <h1 class="SectionTitles" style="width:1100px; ">Give the plant a "big mouth"</h1>
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  <h1 class="SectionTitles" style="width:245px;">Overview</h1>
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Due to time reasons, we have not clone the stomatal regulation gene, AtAHA2, to the expression vector. So our next target would be cloning the AtAHA2 gene and its promoter, GC1, to the expressing vector, transforming that to plants, and testing their abilities of absorbing formaldehyde.
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Because of the limited time, we have not cloned the stomatal regulation gene, <i>AtAHA2</i>, to the expression vectors as we planned to. So our next target would be cloning <i>AtAHA2</i> gene and its promoter, GC1, to the expressing vector, transforming that to plants, and testing their abilities of absorbing formaldehyde.
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The chloroplast is a pivotal organelle in plant cells and eukaryotic algae to carry out photosynthesis, which provides the primary source of the world’s food. The expression of foreign genes in chloroplasts offers several a dvantages over their expression in the nucleus: high-level expression, transgene stacking in operons and a lack of epigenetic interference allowing stable transgene expression. In addition, transgenic chloroplasts are generally not transmitted through pollen grains because of the cytoplasmic localization.  And it meets the requirement of biosafety of iGEM. In the past two decades, great progress in chloroplast engineering has been made. So in the future work, we will tranform the genes into the chloroplast of plants.
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<div align="center"><img style="width:20%;" src="https://static.igem.org/mediawiki/2014/6/6d/Uestc_big.png"></div><br/>
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  <h1 class="SectionTitles" style="width:1100px; ">Chloroplast transformation</h1>
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<p>The chloroplast is a pivotal organelle in plant cells and eukaryotic algae to carry out photosynthesis, which provides the primary source of the world’s food. The expression of foreign genes in chloroplasts offers several advantages over their expression in the nucleus: high-level expression, transgene stacking in operons and a lack of epigenetic interference allowing stable transgene expression. In addition, transgenic chloroplasts are generally not transmitted through pollen grains because of the cytoplasmic localization.  And it meets the requirement of biosafety of iGEM. In the past two decades, great progress in chloroplast engineering has been made. So in the future work, we will tranform the genes into the chloroplast of plants.
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  <h1 class="SectionTitles" style="width:1100px; ">More plants</h1>
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<p>By now our project is still in its basic research stage. And our experiments were on the model plant, tobacco. In the near future we will try it on some ornamental plants. Our super plant is still living in sealed boxes, and they will be cultivated in bigger circumstances, such as rooms, offices, automobile rooms, etc.
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<spam><img src="https://static.igem.org/mediawiki/2014/8/86/Uestc_20141017_104324.jpg" style="width: 47%;">
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<img src="https://static.igem.org/mediawiki/2014/7/78/Uestc_20141017_104401.jpg" style="width: 47%;"></spam><br/><br/>
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<h1 class="SectionTitles" style="width:1100px; ">Pollen abortion detection</h1>
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<p>We brought in cysteine protease expression part which caused pollen abortion. We ensured biosafety of transgenic modified organism in this way. However, there is not enough time to wait until transgenic tobacco flowers. And this will be our future work.
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By now our project is still in its basic research stage. So we experiments was on the model plant, tobacco. Next time we will try it on some ornamental plants.
 
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Latest revision as of 02:54, 18 October 2014

UESTC-China