Team:Kyoto

From 2014.igem.org

(Difference between revisions)
 
(31 intermediate revisions not shown)
Line 1: Line 1:
{{Kyoto/css/top}}
{{Kyoto/css/top}}
-
{{Kyoto/small_countdown|day=11|popup=俺たちのWIKI FREEZE}}
+
{{Kyoto/small_countdown}}
<html>
<html>
<div class="kyoto-centering-wrapper"><div id="kyoto-top-background"></div></div>
<div class="kyoto-centering-wrapper"><div id="kyoto-top-background"></div></div>
<div id="kyoto-top">
<div id="kyoto-top">
-
   <div id="kyoto-top-dms" class="kyoto-top-theme">
+
   <div id="kyoto-top-magnet" class="kyoto-top-theme">
       <div class="kyoto-fade">
       <div class="kyoto-fade">
-
         <div id="kyoto-top-abst-dms">
+
        <h1 id="kyoto-title-magnet"><a href="https://2014.igem.org/Team:Kyoto/Project/Magnetosome_Formation" title="Magnetosome Formation"></a></h1>
 +
         <div id="kyoto-top-abst-magnet">
             <div class="kyoto-top-abst-blank"></div>
             <div class="kyoto-top-abst-blank"></div>
             <h2>Project Overview</h2>
             <h2>Project Overview</h2>
-
             <p>Generating cloud. This is our project aim. You might think we are dreaming, but, we found a clue in some scientists’ words. In wetlands or sea, specific marine bacteria and coral produce dimethyl sulfide (DMS), which becomes a Cloud Condensation Nuclei (CNN), we thought we could produce cloud by creating <i>E.coli</i> which synthesizes DMS. To realize this, we consist a biosynthetic pathway of DMS in <i>E.coli</i> by introducing genes from <i>Fragilariopsis cylindrus</i> and <i>Ruegeria pomeroyi</i>. We use DMS detecting tube and High Performance Liquid Chromatography (HPLC) to confirm the function. Don’t you think it is fantastic if the small organism, <i>E. coli</i>, creates something big, cloud? And this project may have the possibility to solve environmental problems.</p>
+
             <p>If we are able to move <i>E. coli</i> to the proper places, the achievements of synthetic biology will be more useful. For example, bioremediation, drug delivery, making <i>E. coli</i> pellet without centrifuge, etc. In order to realize this dream, we focused on the magnetosome: a magnetic organelle of magnetotactic bacteria. It consists of a lipid bilayer and a  magnet. <i>E. coli</i> with magnetosome will be moved by magnetism. To realize this amazing <i>E. coli</i>, we started from making the magnetosome. Finally, we detected magnetosome-like vesicles in our transformants!
-
             <h3><a href="https://2014.igem.org/Team:Kyoto/Project/DMS_Synthesis">Read More... Click Here!</a></h3>
+
</p>
-
         </div>                               <!-- close ".kyoto-top-abst#kyoto-top-abst-dms" -->
+
             <h3 style="text-indent: 30px;"><a href="https://2014.igem.org/Team:Kyoto/Project/Magnetosome_Formation" style="color: #88ffee;">Read More... Click Here!</a></h3>
 +
         </div>                                           <!-- close "#kyoto-top-abst-magnet" -->
         <img src="https://static.igem.org/mediawiki/2014/7/7d/Kyoto-Magchara.png">
         <img src="https://static.igem.org/mediawiki/2014/7/7d/Kyoto-Magchara.png">
-
       </div>                                   <!-- close div.kyoto-fade -->
+
       </div>                                               <!-- close div.kyoto-fade -->
-
   </div>                                             <!-- close "#kyoto-top-dms" -->
+
   </div>                                                         <!-- close "#kyoto-top-magnet" -->
-
   <div id="kyoto-top-magnet" class="kyoto-top-theme">
+
   <div id="kyoto-top-dms" class="kyoto-top-theme">
       <div class="kyoto-fade">
       <div class="kyoto-fade">
-
         <div id="kyoto-top-abst-magnet">
+
        <h1 id="kyoto-title-dms"><a href="https://2014.igem.org/Team:Kyoto/Project/DMS_Synthesis" title="DMS Synthesis"></a></h1>
 +
         <div id="kyoto-top-abst-dms">
             <div class="kyoto-top-abst-blank"></div>
             <div class="kyoto-top-abst-blank"></div>
             <h2>Project Overview</h2>
             <h2>Project Overview</h2>
-
             <p>We're dreaming of keeping <i>E. coil</i> in hand; moving them here and there. Magneto.coli will realize it. A magnetosome is an organelle seen in magnetotactic bacteria; storing magnetite in it. We believe <i>E. coil</i> with a magnetosome can be moved by magnetism
+
             <p>Generating cloud. This is our project aim. You might think we are dreaming, but, we found a clue in some scientists' words. In wetlands or sea, specific marine bacteria and coral produce dimethyl sulfide (DMS), which becomes a Cloud Condensation Nuclei (CCN), we thought we could produce cloud by creating <i>E. coli</i> which synthesizes DMS. To realize this, we consist a biosynthetic pathway of DMS in <i>E. coli</i> by introducing genes from <i>Fragilariopsis cylindrus</i> and <i>Ruegeria pomeroyi</i>. We use DMS detecting tube and High Performance Liquid Chromatography (HPLC) to confirm the function. Don't you think it is fantastic if the small organism, <i>E. coli</i>, creates something big, cloud? And this project may have the possibility to solve environmental problems.</p>
-
. To make magetosomes in <i>E. coil</i>, at least two processes are required; vesicle formation and uptake of iron in it. It had been unclear what genes were necessary for them in <i>E. coil</i>. We focused on vesicle formation through this project, and found <i>mamL/Q</i>, derived from <i>Magnetospirillum magnetotacticum</i>, played an important role in vesicle formation. We did detect organelles in <i>mamL/Q</i>-induced <i>E. coil</i>.</p>
+
             <h3><a href="https://2014.igem.org/Team:Kyoto/Project/DMS_Synthesis" style="color: #ffdd77">Read More... Click Here!</a></h3>
-
             <h3><a href="https://2014.igem.org/Team:Kyoto/Project/Magnetosome_Formation">Read More... Click Here!</a></h3>
+
         </div>                               <!-- close "#kyoto-top-abst-dms" -->
-
         </div>                                           <!-- close ".kyoto-top-abst#kyoto-top-abst-magnet" -->
+
         <img src="https://static.igem.org/mediawiki/2014/b/b3/Dmschar.png">
         <img src="https://static.igem.org/mediawiki/2014/b/b3/Dmschar.png">
-
       </div>                                               <!-- close div.kyoto-fade -->
+
       </div>                                   <!-- close div.kyoto-fade -->
-
   </div>                                                         <!-- close "#kyoto-top-magnet" -->
+
   </div>                                             <!-- close "#kyoto-top-dms" -->
   <div class="kyoto-centering-wrapper" id="kyoto-top-icon">
   <div class="kyoto-centering-wrapper" id="kyoto-top-icon">
       <a href="#" id="kyoto-top-logo"></a>
       <a href="#" id="kyoto-top-logo"></a>
Line 33: Line 35:
             <li><a id="kyoto-top-menu-project" href="https://2014.igem.org/Team:Kyoto/Project" title="Project"></a></li>
             <li><a id="kyoto-top-menu-project" href="https://2014.igem.org/Team:Kyoto/Project" title="Project"></a></li>
             <li><a id="kyoto-top-menu-MM" href="https://2014.igem.org/Team:Kyoto/Material_and_Method" title="Material & Method"></a></li>
             <li><a id="kyoto-top-menu-MM" href="https://2014.igem.org/Team:Kyoto/Material_and_Method" title="Material & Method"></a></li>
-
             <li><a id="kyoto-top-menu-note" href="https://2014.igem.org/Team:Kyoto/Notebook/DMS_Synthesis" title="Notebook"></a></li>
+
             <li><a id="kyoto-top-menu-note" href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation" title="Notebook"></a></li>
         </ul>
         </ul>
       </div>                                                      <!-- close "#kyoto-top-menu-left.top-menu" -->
       </div>                                                      <!-- close "#kyoto-top-menu-left.top-menu" -->

Latest revision as of 02:48, 18 October 2014

count down

Project Overview

If we are able to move E. coli to the proper places, the achievements of synthetic biology will be more useful. For example, bioremediation, drug delivery, making E. coli pellet without centrifuge, etc. In order to realize this dream, we focused on the magnetosome: a magnetic organelle of magnetotactic bacteria. It consists of a lipid bilayer and a magnet. E. coli with magnetosome will be moved by magnetism. To realize this amazing E. coli, we started from making the magnetosome. Finally, we detected magnetosome-like vesicles in our transformants!

Read More... Click Here!

Project Overview

Generating cloud. This is our project aim. You might think we are dreaming, but, we found a clue in some scientists' words. In wetlands or sea, specific marine bacteria and coral produce dimethyl sulfide (DMS), which becomes a Cloud Condensation Nuclei (CCN), we thought we could produce cloud by creating E. coli which synthesizes DMS. To realize this, we consist a biosynthetic pathway of DMS in E. coli by introducing genes from Fragilariopsis cylindrus and Ruegeria pomeroyi. We use DMS detecting tube and High Performance Liquid Chromatography (HPLC) to confirm the function. Don't you think it is fantastic if the small organism, E. coli, creates something big, cloud? And this project may have the possibility to solve environmental problems.

Read More... Click Here!