Team:Kyoto

From 2014.igem.org

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Latest revision as of 02:48, 18 October 2014

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Project Overview

If we are able to move E. coli to the proper places, the achievements of synthetic biology will be more useful. For example, bioremediation, drug delivery, making E. coli pellet without centrifuge, etc. In order to realize this dream, we focused on the magnetosome: a magnetic organelle of magnetotactic bacteria. It consists of a lipid bilayer and a magnet. E. coli with magnetosome will be moved by magnetism. To realize this amazing E. coli, we started from making the magnetosome. Finally, we detected magnetosome-like vesicles in our transformants!

Project Overview

Generating cloud. This is our project aim. You might think we are dreaming, but, we found a clue in some scientists' words. In wetlands or sea, specific marine bacteria and coral produce dimethyl sulfide (DMS), which becomes a Cloud Condensation Nuclei (CCN), we thought we could produce cloud by creating E. coli which synthesizes DMS. To realize this, we consist a biosynthetic pathway of DMS in E. coli by introducing genes from Fragilariopsis cylindrus and Ruegeria pomeroyi. We use DMS detecting tube and High Performance Liquid Chromatography (HPLC) to confirm the function. Don't you think it is fantastic if the small organism, E. coli, creates something big, cloud? And this project may have the possibility to solve environmental problems.

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+   <div id="kyoto-top-magnet" class="kyoto-top-theme">
+      <div class="kyoto-fade">
+         <h1 id="kyoto-title-magnet"><a href="https://2014.igem.org/Team:Kyoto/Project/Magnetosome_Formation" title="Magnetosome Formation"></a></h1>
+         <div id="kyoto-top-abst-magnet">
+            <div class="kyoto-top-abst-blank"></div>
+            <h2>Project Overview</h2>
+            <p>If we are able to move <i>E. coli</i> to the proper places, the achievements of synthetic biology will be more useful. For example, bioremediation, drug delivery, making <i>E. coli</i> pellet without centrifuge, etc. In order to realize this dream, we focused on the magnetosome: a magnetic organelle of magnetotactic bacteria. It consists of a lipid bilayer and a  magnet. <i>E. coli</i> with magnetosome will be moved by magnetism. To realize this amazing <i>E. coli</i>, we started from making the magnetosome. Finally, we detected magnetosome-like vesicles in our transformants!
+</p>
+            <h3 style="text-indent: 30px;"><a href="https://2014.igem.org/Team:Kyoto/Project/Magnetosome_Formation" style="color: #88ffee;">Read More... Click Here!</a></h3>
+         </div>                                            <!-- close "#kyoto-top-abst-magnet" -->
+         <img src="https://static.igem.org/mediawiki/2014/7/7d/Kyoto-Magchara.png">
+      </div>                                                <!-- close div.kyoto-fade -->
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     <div id="kyoto-top-dms" class="kyoto-top-theme">
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+         <h1 id="kyoto-title-dms"><a href="https://2014.igem.org/Team:Kyoto/Project/DMS_Synthesis" title="DMS Synthesis"></a></h1>
           <div id="kyoto-top-abst-dms">
              <div class="kyoto-top-abst-blank"></div>
              <h2>Project Overview</h2>
-             <p>This year, we have two projects. The first project is DMS (dimethylsulfide) synthesis. DMS is known to be a precursor of the cloud condensation nucleus. Some researchers say that DMS greatly affects the climate. Although the existing researches have already lined up the candidate genes of the DMS biosynthesis pathway, most of them are still unconfirmed. Therefore, we tried to confirm them by introducing 5 candidate genes in <i>Fragilariopsis cylindrus</i>; a diatom, into <i>Escherichia coli</i>.</p>
+             <p>Generating cloud. This is our project aim. You might think we are dreaming, but, we found a clue in some scientists' words. In wetlands or sea, specific marine bacteria and coral produce dimethyl sulfide (DMS), which becomes a Cloud Condensation Nuclei (CCN), we thought we could produce cloud by creating <i>E. coli</i> which synthesizes DMS. To realize this, we consist a biosynthetic pathway of DMS in <i>E. coli</i> by introducing genes from <i>Fragilariopsis cylindrus</i> and <i>Ruegeria pomeroyi</i>. We use DMS detecting tube and High Performance Liquid Chromatography (HPLC) to confirm the function. Don't you think it is fantastic if the small organism, <i>E. coli</i>, creates something big, cloud? And this project may have the possibility to solve environmental problems.</p>
-             <h3><a href="https://2014.igem.org/Team:Kyoto/Project/DMS_Synthesis">Read More... Click Here!</a></h3>
+             <h3><a href="https://2014.igem.org/Team:Kyoto/Project/DMS_Synthesis" style="color: #ffdd77">Read More... Click Here!</a></h3>
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+         <img src="https://static.igem.org/mediawiki/2014/b/b3/Dmschar.png">
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-            </table>
-            <h2>Project Overview</h2>
-            <p>The second project is magnetosome formation. Magnetosome is a specific organelle in magnetotactic bacteria. Although many researchers have focused on it for its unique feature; magnetosome detect magnetic force, they have discovered only magnetotactic bacteria to have this ability so far. So we tried to form the Magnetosome in <i>E. coli</i> by introducing 4 operons which has been lined up recently as the sufficient genes to make magnetosome in <i>Magnetospirillum gryphiswaldense</i>.</p>
-            <h3><a href="https://2014.igem.org/Team:Kyoto/Project/Magnetosome_Formation">Read More... Click Here!</a></h3>
-         </div>                                            <!-- close ".kyoto-top-abst#kyoto-top-abst-magnet" -->
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-             <li><a id="kyoto-top-menu-note" href="https://2014.igem.org/Team:Kyoto/Notebook/DMS_Synthesis" title="Notebook"></a></li>
+             <li><a id="kyoto-top-menu-note" href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation" title="Notebook"></a></li>
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     <ul>
-       <li><a href="http://www.kyoto-u.ac.jp/en" target="_blank" title="Kyoto Univ."><img src="https://static.igem.org/mediawiki/2014/1/12/Kyoto-Ku02.png"></a></li>
+       <li id="kyoto-top-sponsor-ku"><a href="http://www.kyoto-u.ac.jp/en" target="_blank" title="Kyoto Univ."><img src="https://static.igem.org/mediawiki/2014/1/12/Kyoto-Ku02.png"></a></li>
-       <li><a href="http://www.promega.jp/" target="_blank" title="Promega"><img src="https://static.igem.org/mediawiki/2014/d/d6/Kyoto-Promegalogo02.png"></a></li>
+       <li id="kyoto-top-sponsor-promega"><a href="http://www.promega.jp/" target="_blank" title="Promega"><img src="https://static.igem.org/mediawiki/2014/d/d6/Kyoto-Promegalogo02.png"></a></li>
-       <li><a href="http://www.cosmobio.co.jp/index_e.asp" target="_blank" title="COSMO BIO"><img src="https://static.igem.org/mediawiki/2014/7/76/Kyoto-Cosmobio.png"></a></li>
+       <li id="kyoto-top-sponsor-cosmo"><a href="http://www.cosmobio.co.jp/index_e.asp" target="_blank" title="COSMO BIO"><img src="https://static.igem.org/mediawiki/2014/7/76/Kyoto-Cosmobio.png"></a></li>
-       <li><a href="http://www.idt.com/" target="_blank" title="IDT"><img src="https://static.igem.org/mediawiki/2014/8/89/Kyoto-IDT.png"></a><a href="http://www.mbl.co.jp/e/index.html" target="_blank" title="MBL"><img src="https://static.igem.org/mediawiki/2014/8/87/MBL02.png"></a></li>
+       <li id="kyoto-top-sponsor-im"><a href="http://www.idt.com/" target="_blank" title="IDT"><img src="https://static.igem.org/mediawiki/2014/8/89/Kyoto-IDT.png" style="padding: 28px 0px 5px 0px;"></a><a href="http://www.mbl.co.jp/e/index.html" target="_blank" title="MBL"><img src="https://static.igem.org/mediawiki/2014/8/87/MBL02.png" style="padding: 5px 0px 18px 0px;"></a></li>
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