Team:Bordeaux/Parts/BBa K1317004

From 2014.igem.org

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Link to the registry of Standard Parts: [http://parts.igem.org/Part:BBa_K1317004 http://parts.igem.org/Part:BBa_K1317004]
Link to the registry of Standard Parts: [http://parts.igem.org/Part:BBa_K1317004 http://parts.igem.org/Part:BBa_K1317004]
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[[File:Bdx2014 ELP-RFP.PNG|800px|thumb|left]]
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MAP of ELP-RLP in pSB1C3. The final assembling could not be performed because we did not have the time to do it.<br />
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The ELP presents specific properties: it precipitates at high temperature. This property can be used for thermal cycling purification for instance. By successive cycles of temperature, ELP can be purified from a mix of proteins. This special property has been used to create a composite part for the iGEM registry. It is a proof of concept of the use of this part as a tool for protein purification by fusing ELP to another protein. The protein used as an example is RFP, to ease vizualisation of the process.<br />
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This part is a composite part made of BBa_K1317003 and BBa_E1010 (highly engineered RFP)
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{{:Team:Bordeaux/Pied}}
{{:Team:Bordeaux/Pied}}

Latest revision as of 02:46, 18 October 2014

Link to the registry of Standard Parts: [http://parts.igem.org/Part:BBa_K1317004 http://parts.igem.org/Part:BBa_K1317004]

Bdx2014 ELP-RFP.PNG

MAP of ELP-RLP in pSB1C3. The final assembling could not be performed because we did not have the time to do it.

The ELP presents specific properties: it precipitates at high temperature. This property can be used for thermal cycling purification for instance. By successive cycles of temperature, ELP can be purified from a mix of proteins. This special property has been used to create a composite part for the iGEM registry. It is a proof of concept of the use of this part as a tool for protein purification by fusing ELP to another protein. The protein used as an example is RFP, to ease vizualisation of the process.

This part is a composite part made of BBa_K1317003 and BBa_E1010 (highly engineered RFP)





























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