Team:ETH Zurich/labblog/20140709meet

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== Title ?==
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== Week 7: Human practice planning, Plasmid assembly running, Logo design  ==  
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= Wednesday, July 9th =
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==== Wednesday, July 9th ====
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=== Title ? ===
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Digest with instable FseI did not work, got psEVA591 from Andreas, no longer need psEVA291 and psEVA561
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* We have a logo !
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[[File:ETH_Zurich_mosaicoli.png|300px]]
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*In the lab, we did some :
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**Plasmid preparation
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**Sequencing
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**Digests
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**Purification of backbone fragments needed for GA
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Transformation of 591, plating on tet.
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*On the modeling side, we tried to estimate integrase parameters from the paper from Bonnet et al. <sup>[[Team:ETH_Zurich/project/references#refEmergence|[9]]]</sup>, more particularly with the figure S4 :
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[[File:ETH Zurich Bonnet figure.PNG|center|500px|thumb|Data we are using from the Bonnet et al. [[Team:ETH_Zurich/project/references#refEmergence| <sup>[9]</sup>]] paper]]
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Preculture of 18941 (5ml) and 44456 (20ml) from addgene), shake overnight
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We are trying several strategies : minimization of the error function and Markov Chain Monte Carlo.
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* We have a precise plan for our human practice project. We would like to study the emergence of complexity in many different fields and investigate how people deal with it. We will do this with interviews of experts in different fields, and with a survey for a wider outreach. We will also reach younger people by organizing talks in schools. We will finally give our own advice on the question by writing an essay on the subject and linking it to our experience with Mosaicoli.
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Latest revision as of 02:45, 18 October 2014