Team:Freiburg/Content/Notebook

From 2014.igem.org

(Difference between revisions)
 
(10 intermediate revisions not shown)
Line 1: Line 1:
<html>
<html>
<body>
<body>
 +
 +
 +
<h1>Cloning</h1>
 +
 +
<p>The protocols for our methods can be found here.</p>
 +
<p>During our experiments, we conducted a lot of PCRs with different Primers. In order to stay on top of things, we numbered Primers, PCRs and constructs. You can download both lists with detailed information about Primer composition and about PCRs in the following documents:</p>
 +
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/a/a6/2014Freiburg_Labbook_Digests.JPG">
 +
        <figcaption>
 +
<p class="header">Fig.1: Project overview.</p>
 +
        </figcaption>
 +
    </figure>
 +
 +
</br>
 +
<figure>
 +
        <img src="https://static.igem.org/mediawiki/2014/e/e7/Freiburg2014_PCRs_labbook.JPG">
 +
        <figcaption>
 +
<p class="header">Fig.1: Project overview.</p>
 +
        </figcaption>
 +
    </figure>
 +
Line 19: Line 41:
<figure>
<figure>
-
         <img style="width: 200px;" style="width: 200px;" src="https://static.igem.org/mediawiki/2014/0/07/2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg">
+
         <img style="width:400px" style="width: 200px;" src="https://static.igem.org/mediawiki/2014/0/07/2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 38: Line 60:
  <figure>
  <figure>
-
<img style="width: 200px;" style="width: 200px;" src="https://static.igem.org/mediawiki/2014/d/dc/2014Freiburg_140729Testverdau_006%2BSLC%2BHA_PstI_006%2BSLC_PstI.jpg">
+
<img style="width:400px" style="width: 200px;" src="https://static.igem.org/mediawiki/2014/d/dc/2014Freiburg_140729Testverdau_006%2BSLC%2BHA_PstI_006%2BSLC_PstI.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 81: Line 103:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/8/81/2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/8/81/2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 97: Line 119:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 110: Line 132:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 141: Line 163:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 157: Line 179:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/9/93/2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/93/2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg">
<figcaption>
<figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 170: Line 192:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/0/05/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/05/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 202: Line 224:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/5/5f/2014Freiburg_140811_PCR1%2C2%2C4%2C5%2C6%2C7%2C9.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/5f/2014Freiburg_140811_PCR1%2C2%2C4%2C5%2C6%2C7%2C9.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 218: Line 240:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 230: Line 252:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 259: Line 281:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/5/5e/2014Freiburg_140811_PCR1%2C17%2C18.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/5e/2014Freiburg_140811_PCR1%2C17%2C18.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 275: Line 297:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 288: Line 310:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 319: Line 341:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 335: Line 357:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 348: Line 370:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/0/00/2014Freiburg_140923_Testverdau_Gibson_16_1x_Gibson_17%2C31%2C33%2C34_je_3x_a.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/00/2014Freiburg_140923_Testverdau_Gibson_16_1x_Gibson_17%2C31%2C33%2C34_je_3x_a.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 377: Line 399:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/0/07/2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/07/2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 393: Line 415:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/c/c8/2014Freiburg_140729Testverdau_084%2BSLC_SmaI.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/c8/2014Freiburg_140729Testverdau_084%2BSLC_SmaI.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 437: Line 459:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 450: Line 472:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 481: Line 503:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/9/93/2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/93/2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 497: Line 519:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
<figcaption>
<figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 510: Line 532:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/c/c0/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_2_b.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/c0/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_2_b.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 540: Line 562:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 556: Line 578:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/60/2014Freiburg_140820_PCR_7_2x_PCR_28_2x.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/60/2014Freiburg_140820_PCR_7_2x_PCR_28_2x.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 569: Line 591:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/0/05/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/05/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 598: Line 620:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/b/b7/2014Freiburg_140719_PCR_fuer_Blaulicht_puro_pKM297_pKM022_pKM292_erfolgreich_55_60_Grad_c1_egfp_plxv_verdau.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/b7/2014Freiburg_140719_PCR_fuer_Blaulicht_puro_pKM297_pKM022_pKM292_erfolgreich_55_60_Grad_c1_egfp_plxv_verdau.jpg">
         <figcaption>
         <figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 614: Line 636:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 627: Line 649:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140728_Testverdau_pMIG_CMV_1-4_pMIG_MCS_unverdaut2x_verdaut_6x.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140728_Testverdau_pMIG_CMV_1-4_pMIG_MCS_unverdaut2x_verdaut_6x.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 657: Line 679:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/0/09/2014Freiburg_140811_PCR10%2C14%2C15%2C17%2C18%2C19%2C20.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/09/2014Freiburg_140811_PCR10%2C14%2C15%2C17%2C18%2C19%2C20.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 673: Line 695:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/1/19/2014Freiburg_140822_Digest8%2C_11%2C_10.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/1/19/2014Freiburg_140822_Digest8%2C_11%2C_10.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 686: Line 708:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/5/51/2014Freiburg_140825_Testverdau_rz003_KpnI_2x_pSAM_Xba_3x_ls003_PstI_2x_ls001_Bbs_EcoRI_2x_zl005_EcoRV_5x_pMIG%2BSyn_PstI_5x_pMIG-Xba_Xba%2CSpe_5x_b.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/51/2014Freiburg_140825_Testverdau_rz003_KpnI_2x_pSAM_Xba_3x_ls003_PstI_2x_ls001_Bbs_EcoRI_2x_zl005_EcoRV_5x_pMIG%2BSyn_PstI_5x_pMIG-Xba_Xba%2CSpe_5x_b.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 715: Line 737:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/0/06/2014Freiburg_140709_pMIG_vor_Gelelution_3-6b%2CplXV9a-12a.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/06/2014Freiburg_140709_pMIG_vor_Gelelution_3-6b%2CplXV9a-12a.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 739: Line 761:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/1/1f/2014Freiburg_140713_pMIG_IRES_out_und_pMIG_eGFP_und_IRES_out-Testverdau_.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/1/1f/2014Freiburg_140713_pMIG_IRES_out_und_pMIG_eGFP_und_IRES_out-Testverdau_.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 768: Line 790:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 792: Line 814:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/a/a1/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_a.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/a/a1/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_a.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 823: Line 845:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 844: Line 866:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/c/cb/2014Freiburg_9_10_2014_2_13_33_PM_a.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/c/cb/2014Freiburg_9_10_2014_2_13_33_PM_a.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 876: Line 898:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 892: Line 914:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 905: Line 927:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 934: Line 956:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 950: Line 972:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 963: Line 985:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/2/23/2014Freiburg_9_10_2014_2_13_33_PM_b.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/2/23/2014Freiburg_9_10_2014_2_13_33_PM_b.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 993: Line 1,015:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 1,009: Line 1,031:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 1,022: Line 1,044:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/2/23/2014Freiburg_9_10_2014_2_13_33_PM_b.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/2/23/2014Freiburg_9_10_2014_2_13_33_PM_b.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 1,052: Line 1,074:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 1,068: Line 1,090:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 1,081: Line 1,103:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 1,110: Line 1,132:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 1,126: Line 1,148:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 1,139: Line 1,161:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/0/0f/2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/0f/2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 1,168: Line 1,190:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 1,184: Line 1,206:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 1,197: Line 1,219:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 1,226: Line 1,248:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/8/81/2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/8/81/2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 1,242: Line 1,264:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 1,255: Line 1,277:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 1,284: Line 1,306:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/b/b0/2014Freiburg_140814_PCR_16%2C19%2C20_Verdau_pLXV_MluI_ClaI_Verdau_pcDNA_BamHI_HindIII.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/b0/2014Freiburg_140814_PCR_16%2C19%2C20_Verdau_pLXV_MluI_ClaI_Verdau_pcDNA_BamHI_HindIII.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 1,300: Line 1,322:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 1,313: Line 1,335:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 1,342: Line 1,364:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 1,358: Line 1,380:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 1,371: Line 1,393:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/0/0f/2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/0/0f/2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 1,400: Line 1,422:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR_digest</p>
<p class="header">PCR_digest</p>
Line 1,423: Line 1,445:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/b/b3/2014Freiburg_2014_09_14_Testverdaue_Gibson_32_31.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/b/b3/2014Freiburg_2014_09_14_Testverdaue_Gibson_32_31.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>
Line 1,453: Line 1,475:
<figure>
<figure>
-
         <img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/5/52/2014Freiburg_9_10_2014_2_10_51_PM.jpg">
+
         <img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/52/2014Freiburg_9_10_2014_2_10_51_PM.jpg">
         <figcaption>
         <figcaption>
<p class="header">PCR</p>
<p class="header">PCR</p>
Line 1,469: Line 1,491:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg">
<figcaption>
<figcaption>
<p class="header">Digest</p>
<p class="header">Digest</p>
Line 1,482: Line 1,504:
  <figure>
  <figure>
-
<img style="width: 200px;" src="https://static.igem.org/mediawiki/2014/5/52/2014Freiburg_9_10_2014_2_10_51_PM.jpg">
+
<img style="width:400px" src="https://static.igem.org/mediawiki/2014/5/52/2014Freiburg_9_10_2014_2_10_51_PM.jpg">
<figcaption>
<figcaption>
<p class="header">Testdigest</p>
<p class="header">Testdigest</p>

Latest revision as of 02:44, 18 October 2014

Cloning

The protocols for our methods can be found here.

During our experiments, we conducted a lot of PCRs with different Primers. In order to stay on top of things, we numbered Primers, PCRs and constructs. You can download both lists with detailed information about Primer composition and about PCRs in the following documents:

Fig.1: Project overview.


Fig.1: Project overview.

p14rz_002

  • PCR: o14rz_005, o14_rz006; Template pQXCIN-SLC7A1
  • Digest: pKM006, EcoRI + NotI; PCR-product
  • Testdigest with PstI in Cutsmart, expected bands: 5,9; 0,7

PCR

Testdigest

p14rz_003

  • PCR 3
  • Digest 3, 4
  • Testdigest with PstI + SpeI in Cutsmart, expected bands: 4,1;2,1;1,8

PCR

Digest

Testdigest

p14rz_004

  • PCR 21,22
  • Digest -
  • Testdigest with PstI in Cutsmart, expected bands: 4,1;3,2

PCR

PCR

Testdigest

p14rz_005

  • PCR 4,5
  • Digest 5
  • Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,3

PCR

Digest

Testdigest

p14rz_006

  • PCR 17,18
  • Digest 5
  • Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,4

PCR

Digest

Testdigest

p14rz_007

  • PCR 37,38
  • Digest 21
  • Testdigest with PstI in Cutsmart, expected bands: 4,1;2,2;1,7

PCR

Digest

Testdigest

p14rz_008

  • PCR blue light receptor; Template: pQXCIN-SLC7A1
  • Digest pKM006 EcoRI, NotI
  • Testdigest with SmaI in Cutsmart, expected bands: 5,7

PCR

Testdigest

p14rz_009

  • PCR 39,40
  • Digest 5
  • Testdigest with BamHI in Cutsmart, expected bands: 3,0;2,7

Digest

Testdigest

p14rz_010

  • PCR 23,24
  • Digest 12
  • Testdigest with PstI in Cutsmart, expected bands:3,1;2,3;0,56

PCR

PCR

Testdigest

p14ls_003

  • PCR 7,9,10
  • Digest -
  • Testdigest with KpnI in Cutsmart, expected bands: 4,9; 1,3; 0,4

PCR

Digest

Testdigest

p14vv_001

  • PCR Primers vv003,vv004; Template: pMIG
  • Digest pMIG EcoRI,NotI; C1-eGFP EcoRI,NotI
  • Testdigest with NgoMIV in Cutsmart, expected bands: 5,7; 0,6

Digest

Digest

Testdigest

p14vv_002

  • PCR -
  • Digest 8,11
  • Testdigest with PstI in Cutsmart, expected bands: 3,2;2,1;1,4

PCR

Digest

Testdigest

p14vv_003

  • PCR pMIG with primer vv003+vv004-
  • Digest xx
  • Testdigest with KpnI in Cutsmart, expected bands: 3,5;1,5

PCR

Testdigest

p14vv_006

  • PCR 26
  • Digest 13
  • Testdigest with NgoMIV in Cutsmart, expected bands: 3,5;1,9;0,95,0,6

PCR

Testdigest

p14vv_007

  • PCR 27
  • Digest 14
  • Testdigest with NgoMIV in Cutsmart, expected bands: 3,5; 1,3; 0,97; 0,66

PCR

Testdigest

p14vv_008

  • PCR 43
  • Digest 22
  • Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 1,4 0,75

PCR

Digest

Testdigest

p14vv_009

  • PCR 44
  • Digest 22
  • Testdigest with EcoRV+MluI in Cutsmart, expected bands: 3,4; 1,43; 0,75

PCR

Digest

Testdigest

p14vv_010

  • PCR 45
  • Digest 22
  • Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 2,2

PCR

Digest

Testdigest

p14vv_012

  • PCR 46
  • Digest 22
  • Testdigest with EcoRV in Cutsmart, expected bands: 4,7;3,4

PCR

Digest

Testdigest

p14vv_013

  • PCR 47,48,49
  • Digest 22
  • Testdigest with NgoMIV in Cutsmart, expected bands: 3,4;2,0;1,3;0,2

PCR

Digest

Testdigest

p14vv_014

  • PCR 50,51
  • Digest 22
  • Testdigest with NgoMIV in Cutsmart, expected bands: 4,1; 1,56; 0,66

PCR

Digest

Testdigest

p14zl_010

  • PCR 1,2
  • Digest 1
  • Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,4;1,1

PCR

Digest

Testdigest

p14zl_011

  • PCR 19,20
  • Digest 1
  • Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,0;1,3;0,43

PCR

Digest

Testdigest

p14zl_016

  • PCR 52,53
  • Digest 22
  • Testdigest with HindIII in Cutsmart, expected bands: 5,7;2,3;1,7

PCR

Digest

Testdigest

p14zl_017

  • PCR 64,65
  • Digest 24
  • testdigest with NgoMIV in Cutsmart, expected bands: 5,4;2,9

PCR_digest

Testdigest

p14zl_020

  • PCR 59,60
  • Digest 24
  • testdigest with BamHI-HF, NgoMIVin Cutsmart, expected bands: 4,7; 2,4; 1,8

PCR

Digest

Testdigest