"
Team:Wageningen UR/overview/parts
From 2014.igem.org
(Difference between revisions)
| (15 intermediate revisions not shown) | |||
| Line 4: | Line 4: | ||
<html> | <html> | ||
<div class="menu-head"> | <div class="menu-head"> | ||
| - | <h4><a href="#header"> | + | <h4><a href="#header">BioBricks</a></h4> |
</div> | </div> | ||
| Line 12: | Line 12: | ||
<html> | <html> | ||
| - | <h1> | + | <h1>BioBricks</h1><br/> |
<p>In the table below, all new BioBricks can be found. Each BioBrick is sent in pSB1C3, unless mentioned otherwise. </p> | <p>In the table below, all new BioBricks can be found. Each BioBrick is sent in pSB1C3, unless mentioned otherwise. </p> | ||
<br/> | <br/> | ||
| + | |||
| + | <figure> | ||
| + | <figcaption>Table 1. BioBricks</figcaption> | ||
<ul class="table"> | <ul class="table"> | ||
<table> | <table> | ||
| Line 68: | Line 71: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493502">BBa_K1493502</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493502">BBa_K1493502</a> </td> | ||
| - | <td> | + | <td>pCI/lac+GFP</td> |
| - | <td>GFP controlled by | + | <td>GFP controlled by pCI/lac</td> |
<td>Reporter</td> | <td>Reporter</td> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K909012">BBa_K909012</a> <a class="soft_link" href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K909012">BBa_K909012</a> <a class="soft_link" href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td> | ||
| Line 75: | Line 78: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493503">BBa_K1493503</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493503">BBa_K1493503</a> </td> | ||
| - | <td> | + | <td>pCI/tet+GFP</td> |
| - | <td>GFP controlled by | + | <td>GFP controlled by pCI/tet</td> |
<td>Reporter</td> | <td>Reporter</td> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K909013">BBa_K909013</a> <a class="soft_link" href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K909013">BBa_K909013</a> <a class="soft_link" href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td> | ||
| Line 89: | Line 92: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493510">BBa_K1493510</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493510">BBa_K1493510</a> </td> | ||
| - | <td>pRHA+ | + | <td>pRHA+CIλ</td> |
| - | <td>Repressor protein | + | <td>Repressor protein CIλ controlled by pRha</td> |
<td>Composite</td> | <td>Composite</td> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K914003">BBa_K914003</a> <a class="soft_link" href="http://parts.igem.org/Part:BBa_P0451">BBa_P0451</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K914003">BBa_K914003</a> <a class="soft_link" href="http://parts.igem.org/Part:BBa_P0451">BBa_P0451</a> </td> | ||
| Line 110: | Line 113: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493540">BBa_K1493540</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493540">BBa_K1493540</a> </td> | ||
| - | <td>pRHA+ | + | <td>pRHA+CIλ +LacI</td> |
| - | <td>Repressor proteins | + | <td>Repressor proteins CIλ and LacI controlled by pRha</td> |
<td>Composite</td> | <td>Composite</td> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K914003">BBa_K914003</a> <a class="soft_link" href="http://parts.igem.org/Part:BBa_S0103">BBa_S0103</a> <a class="soft_link" href="http://parts.igem.org/Part:BBa_P0412">BBa_P0412</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K914003">BBa_K914003</a> <a class="soft_link" href="http://parts.igem.org/Part:BBa_S0103">BBa_S0103</a> <a class="soft_link" href="http://parts.igem.org/Part:BBa_P0412">BBa_P0412</a> </td> | ||
| Line 117: | Line 120: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493550">BBa_K1493550</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493550">BBa_K1493550</a> </td> | ||
| - | <td>pRHA+ | + | <td>pRHA+CI labda+TetR</td> |
| - | <td>Repressor proteins | + | <td>Repressor proteins CIλ and TetR controlled by pRha</td> |
<td>Composite</td> | <td>Composite</td> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K914003">BBa_K914003</a> <a class="soft_link" href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K914003">BBa_K914003</a> <a class="soft_link" href="http://parts.igem.org/Part:BBa_I13504">BBa_I13504</a> </td> | ||
| Line 124: | Line 127: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493560">BBa_K1493560</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493560">BBa_K1493560</a> </td> | ||
| - | <td>pRHA+ | + | <td>pRHA+CIλ+pcI/tet+GFP</td> |
<td>Kill Switch Input/output</td> | <td>Kill Switch Input/output</td> | ||
<td>Reporter</td> | <td>Reporter</td> | ||
| Line 132: | Line 135: | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493601">BBa_K1493601</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493601">BBa_K1493601</a> </td> | ||
<td>LacI + Kis</td> | <td>LacI + Kis</td> | ||
| - | <td> | + | <td>Kis antitoxin</td> |
<td>Composite</td> | <td>Composite</td> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_R0010">BBa_R0010</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_R0010">BBa_R0010</a> </td> | ||
| Line 138: | Line 141: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493602">BBa_K1493602</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493602">BBa_K1493602</a> </td> | ||
| - | <td>LacI + | + | <td>LacI + Kis</td> |
| - | <td> | + | <td>LacI promoter + Kis antitoxin</td> |
<td>Composite</td> | <td>Composite</td> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_J04500">BBa_J04500</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_J04500">BBa_J04500</a> </td> | ||
| Line 152: | Line 155: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493701">BBa_K1493701</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493701">BBa_K1493701</a> </td> | ||
| - | <td> | + | <td>pCI/lac+TetR+GFP</td> |
<td>Part of toggle switch with reporter</td> | <td>Part of toggle switch with reporter</td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| Line 159: | Line 162: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493702">BBa_K1493702</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493702">BBa_K1493702</a> </td> | ||
| - | <td> | + | <td>pCI/lac+TetR</td> |
<td>Part of toggle switch</td> | <td>Part of toggle switch</td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| Line 173: | Line 176: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493801">BBa_K1493801</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493801">BBa_K1493801</a> </td> | ||
| - | <td> | + | <td>Promoter with Tet operator site</td> |
<td>Promoter repressed by TetR</td> | <td>Promoter repressed by TetR</td> | ||
<td>Regulatory</td> | <td>Regulatory</td> | ||
| Line 180: | Line 183: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493802">BBa_K1493802</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493802">BBa_K1493802</a> </td> | ||
| - | <td> | + | <td>Promoter with Tet operator site</td> |
<td>Promoter repressed by TetR</td> | <td>Promoter repressed by TetR</td> | ||
<td>Regulatory</td> | <td>Regulatory</td> | ||
| Line 187: | Line 190: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493803">BBa_K1493803</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493803">BBa_K1493803</a> </td> | ||
| - | <td> | + | <td>promoter with CIλ and LacI operator sites</td> |
| - | <td>Promoter repressed by | + | <td>Promoter repressed by CIλ and LacI</td> |
<td>Regulatory</td> | <td>Regulatory</td> | ||
<td>-</td> | <td>-</td> | ||
| Line 194: | Line 197: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493804">BBa_K1493804</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493804">BBa_K1493804</a> </td> | ||
| - | <td> | + | <td>promoter with CIλ and Tet operator sites</td> |
| - | <td>Promoter repressed by | + | <td>Promoter repressed by CIλ and TetR</td> |
<td>Regulatory</td> | <td>Regulatory</td> | ||
<td>-</td> | <td>-</td> | ||
| Line 201: | Line 204: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493805">BBa_K1493805</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493805">BBa_K1493805</a> </td> | ||
| - | <td> | + | <td>promoter with CIλ and Tet operator sites</td> |
| - | <td>Promoter repressed by | + | <td>Promoter repressed by CIλ and TetR</td> |
<td>Regulatory</td> | <td>Regulatory</td> | ||
<td>-</td> | <td>-</td> | ||
| Line 208: | Line 211: | ||
<tr> | <tr> | ||
<td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493806">BBa_K1493806</a> </td> | <td><a class="soft_link" href="http://parts.igem.org/Part:BBa_K1493806">BBa_K1493806</a> </td> | ||
| - | <td> | + | <td>promoter with CIλ and LacI operator sites</td> |
| - | <td>Promoter repressed by | + | <td>Promoter repressed by CIλ and LacI</td> |
<td>Regulatory</td> | <td>Regulatory</td> | ||
<td>-</td> | <td>-</td> | ||
| Line 216: | Line 219: | ||
</ul> | </ul> | ||
| + | </figure> | ||
| + | <br> | ||
| + | <p style="float:right"><b>Continue to <a href="https://2014.igem.org/Team:Wageningen_UR/project/fungal_sensing" class="soft_link">Fungal Sensing >></a> </b> | ||
</html> | </html> | ||
{{:Team:Wageningen_UR/templates/footer}} | {{:Team:Wageningen_UR/templates/footer}} | ||
Latest revision as of 02:40, 18 October 2014
BioBricks
In the table below, all new BioBricks can be found. Each BioBrick is sent in pSB1C3, unless mentioned otherwise.
| Part code | Name | Function | Category | BioBricks used |
|---|---|---|---|---|
| BBa_K1493000 | FA regulation system | Promoter only functional in presence of fusaric acid | Regulator | - |
| BBa_K1493002 | FA regulation system | Promoter only functional in presence of fusaric acid | Regulator | - |
| BBa_K1493003 pSB1A2 instead of pSB1C3 |
FA regulation system | "Promoter only functional in presence of fusaric acid, produces GFP when active | Regulator | BBa_I13504 |
| BBa_K1493200 | PfrI | Transcription factor involved in pyoverdine synethesis gene transcription | Transcription factor | BBa_B0034 |
| BBa_K1493300 | methionine-lyase | Converts L-methionine to methanethiol ammonium and 2-oxobutanoate | Enzyme | - |
| BBa_K1493501 | pRha+GFP | GFP controlled by pRha | Reporter | BBa_K914003 BBa_I13504 |
| BBa_K1493502 | pCI/lac+GFP | GFP controlled by pCI/lac | Reporter | BBa_K909012 BBa_I13504 |
| BBa_K1493503 | pCI/tet+GFP | GFP controlled by pCI/tet | Reporter | BBa_K909013 BBa_I13504 |
| BBa_K1493504 | pTet+GFP | GFP controlled by pTet | Reporter | BBa_R0040 BBa_I13504 |
| BBa_K1493510 | pRHA+CIλ | Repressor protein CIλ controlled by pRha | Composite | BBa_K914003 BBa_P0451 |
| BBa_K1493520 | pRHA+LacI | Repressor protein LacI controlled by pRha | Composite | BBa_K914003 BBa_P0412 |
| BBa_K1493530 | pRHA+TetR | Repressor protein TetR controlled by pRha | Composite | BBa_K914003 BBa_P0440 |
| BBa_K1493540 | pRHA+CIλ +LacI | Repressor proteins CIλ and LacI controlled by pRha | Composite | BBa_K914003 BBa_S0103 BBa_P0412 |
| BBa_K1493550 | pRHA+CI labda+TetR | Repressor proteins CIλ and TetR controlled by pRha | Composite | BBa_K914003 BBa_I13504 |
| BBa_K1493560 | pRHA+CIλ+pcI/tet+GFP | Kill Switch Input/output | Reporter | BBa_K914003 BBa_P0451 BBa_K909013 BBa_I13504 |
| BBa_K1493601 | LacI + Kis | Kis antitoxin | Composite | BBa_R0010 |
| BBa_K1493602 | LacI + Kis | LacI promoter + Kis antitoxin | Composite | BBa_J04500 |
| BBa_K1493603 | Epsilon + lacI | Zeta antitoxin | Composite | BBa_R0010 |
| BBa_K1493701 | pCI/lac+TetR+GFP | Part of toggle switch with reporter | Composite | BBa_K909012 BBa_S03518 BBa_I13504 |
| BBa_K1493702 | pCI/lac+TetR | Part of toggle switch | Composite | BBa_K909012 BBa_P0440 |
| BBa_K1493703 | ptet+LacI+GFP | Part of Toggle switch with reporter | Composite | BBa_R0040 BBa_S0100 BBa_I13504 |
| BBa_K1493801 | Promoter with Tet operator site | Promoter repressed by TetR | Regulatory | - |
| BBa_K1493802 | Promoter with Tet operator site | Promoter repressed by TetR | Regulatory | - |
| BBa_K1493803 | promoter with CIλ and LacI operator sites | Promoter repressed by CIλ and LacI | Regulatory | - |
| BBa_K1493804 | promoter with CIλ and Tet operator sites | Promoter repressed by CIλ and TetR | Regulatory | - |
| BBa_K1493805 | promoter with CIλ and Tet operator sites | Promoter repressed by CIλ and TetR | Regulatory | - |
| BBa_K1493806 | promoter with CIλ and LacI operator sites | Promoter repressed by CIλ and LacI | Regulatory | - |
Continue to Fungal Sensing >>
