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Team:Paris Saclay/Notebook/June/30
From 2014.igem.org
(→Monday 30th June) |
(→Monday 30th June) |
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====1 - Culture or the supercompetents:==== | ====1 - Culture or the supercompetents:==== | ||
| - | by Mathias | + | ''by Mathias'' |
* MG1655 | * MG1655 | ||
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====2 - Réhydration of selected biobricks:==== | ====2 - Réhydration of selected biobricks:==== | ||
| - | by Juliette | + | ''by Juliette'' |
* BBQ_I9026 | * BBQ_I9026 | ||
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====3 - Transformations :==== | ====3 - Transformations :==== | ||
| - | by Maher & Mathias | + | ''by Maher & Mathias'' |
We use the DH5α provided by our advisors for the aforementioned Biobricks. | We use the DH5α provided by our advisors for the aforementioned Biobricks. | ||
| Line 38: | Line 38: | ||
* Aliquots of antibiotics : | * Aliquots of antibiotics : | ||
| - | by Marie & Mathieu | + | ''by Marie & Mathieu'' |
Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. | Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. | ||
| Line 45: | Line 45: | ||
* Petri dishes | * Petri dishes | ||
| - | by Romain | + | ''by Romain'' |
Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes. | Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes. | ||
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* Range of gelose with different concentration of agar | * Range of gelose with different concentration of agar | ||
| - | by Arnaud | + | ''by Arnaud'' |
Add in 6 bottles of 500ml 2g of LB (lysogeny broth), and in each bottle add successively 1500mg (15mg/ml), 2000mg (20mg/ml), 2500mg (25mg/ml), 3000mg (30mg/ml), 3500mg (35mg/ml) and 4000mg (40mg/ml) of agar. And just before autoclaving complete each bottle with 100ml of reverse osmosis-purified water. | Add in 6 bottles of 500ml 2g of LB (lysogeny broth), and in each bottle add successively 1500mg (15mg/ml), 2000mg (20mg/ml), 2500mg (25mg/ml), 3000mg (30mg/ml), 3500mg (35mg/ml) and 4000mg (40mg/ml) of agar. And just before autoclaving complete each bottle with 100ml of reverse osmosis-purified water. | ||
Revision as of 19:38, 19 July 2014
Contents |
Monday 30th June
Lab work
1 - Culture or the supercompetents:
by Mathias
- MG1655
- MG1655Z1
1mL of an overnight culture in 100mL of LB.
2 - Réhydration of selected biobricks:
by Juliette
- BBQ_I9026
- BBQ_K592025
- BBQ_K786002
- BBQ_K786003
- BBQ_K778002
- BBQ_K592011 {2 times}
- BBQ_K592009
- BBQ_K1033910 {idem}
- BBQ_J45014
- BBQ_K517003
3 - Transformations :
by Maher & Mathias
We use the DH5α provided by our advisors for the aforementioned Biobricks. Spread on petri dishes with the concentration 10^-1 and 10^-2.
4 - Preparations :
- Aliquots of antibiotics :
by Marie & Mathieu
Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. (Do the same for Kanamycin and Ampicillin)
- Petri dishes
by Romain
Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes.
- Range of gelose with different concentration of agar
by Arnaud
Add in 6 bottles of 500ml 2g of LB (lysogeny broth), and in each bottle add successively 1500mg (15mg/ml), 2000mg (20mg/ml), 2500mg (25mg/ml), 3000mg (30mg/ml), 3500mg (35mg/ml) and 4000mg (40mg/ml) of agar. And just before autoclaving complete each bottle with 100ml of reverse osmosis-purified water.
People there:
- Instructors : Solenne.
- Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
