"
Team:British Columbia/Results
From 2014.igem.org
| Line 18: | Line 18: | ||
<center> | <center> | ||
<video width="920" controls> | <video width="920" controls> | ||
| - | <source src="https:// | + | <source src="https://static.igem.org/mediawiki/2014/5/59/UBC_iGEM_chalcopyrite_biomining_2.mp4" type="video/mp4"> |
Your browser does not support the video tag. | Your browser does not support the video tag. | ||
</video> | </video> | ||
| Line 33: | Line 33: | ||
<center> | <center> | ||
<video width="920" controls> | <video width="920" controls> | ||
| - | <source src="https:// | + | <source src="https://static.igem.org/mediawiki/2014/a/a1/UBC_iGEM_chalcopyrite_biomining_1.mp4" type="video/mp4"> |
Your browser does not support the video tag. | Your browser does not support the video tag. | ||
</video> | </video> | ||
Revision as of 02:37, 18 October 2014
Results
Biomining precipitation assays
Before attempting to demonstrate binding with peptide inserted into the S-Layer, we tested binding with WT cells. After having observed binding, we then hypothesized that the fucose layer in Caulobacter was potentially involved in binding. Since there was a fucos knockout was available, we ere able to confirm fucos binding. Moreover, this observation demonstrated that a precipitation assay was feasible when mixing cells with chalcopyrite.
Figure 1. Wild type and fucos knockout Caulobacter cells mixed with Chalcopyrite.
As a preliminary test, we observed increased Chalcopyrite precipitation with Caulobacter cells expressing a Chalcopyrite binding peptide in the S-layer. While non-quantitative, the data showed noticeable differences in precipitate settle when the peptide expressed in various locations in the S-layer. See the video below.
Figure 2. Various peptides from Curtis et al. are displayed in different positions in the S-layer of Caulobacter cells. The cells were mixed with calcopyrite and precipitate settling was observed.
