Team:UGA-Georgia/Notebook
From 2014.igem.org
(Difference between revisions)
MeganCheng (Talk | contribs) |
|||
(36 intermediate revisions not shown) | |||
Line 7: | Line 7: | ||
<html> | <html> | ||
- | < | + | <style> |
- | + | #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/ | |
+ | <style> | ||
+ | #sddm | ||
+ | { margin: 0; | ||
+ | padding: 0; | ||
+ | z-index: 30} | ||
- | + | #sddm li | |
- | + | { margin: 0; | |
- | + | padding: 0; | |
- | + | list-style: none; | |
- | + | float: left; | |
- | + | font: bold 11px arial} | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | #sddm li a | |
- | + | { display: block; | |
- | + | margin: 0 1px 0 0; | |
+ | padding: 4px 10px; | ||
+ | width: 60px; | ||
+ | background: #5970B2; | ||
+ | color: #FFF; | ||
+ | text-align: center; | ||
+ | text-decoration: none} | ||
- | + | #sddm li a:hover | |
- | + | { background: #49A3FF} | |
- | + | #sddm div | |
- | + | { position: absolute; | |
- | + | visibility: hidden; | |
+ | margin: 0; | ||
+ | padding: 0; | ||
+ | background: #EAEBD8; | ||
+ | border: 1px solid #5970B2} | ||
+ | #sddm div a | ||
+ | { position: relative; | ||
+ | display: block; | ||
+ | margin: 0; | ||
+ | padding: 5px 84px; | ||
+ | width: auto; | ||
+ | white-space: nowrap; | ||
+ | text-align: left; | ||
+ | text-decoration: none; | ||
+ | background: #FEE5AD; | ||
+ | color: #000000; | ||
+ | font: 15px arial} | ||
- | + | #sddm div a:hover | |
- | < | + | { background: #49A3FF; |
+ | color: #FFF} | ||
+ | </style> | ||
+ | <html> | ||
+ | <head> | ||
+ | <style> | ||
+ | body { | ||
+ | background-color:white; | ||
+ | background-image: linear-gradient(90deg, rgba(200,0,0,.5) 50%, transparent 50%), | ||
+ | linear-gradient(rgba(200,0,0,.5) 50%, transparent 50%); | ||
+ | background-size:50px 50px; | ||
+ | </style> | ||
+ | </head> | ||
+ | <body> | ||
+ | <script> | ||
+ | var timeout = 500; | ||
+ | var closetimer = 0; | ||
+ | var ddmenuitem = 0; | ||
- | + | // open hidden layer | |
- | + | function mopen(id) | |
+ | { | ||
+ | // cancel close timer | ||
+ | mcancelclosetime(); | ||
+ | // close old layer | ||
+ | if(ddmenuitem) ddmenuitem.style.visibility = 'hidden'; | ||
+ | // get new layer and show it | ||
+ | ddmenuitem = document.getElementById(id); | ||
+ | ddmenuitem.style.visibility = 'visible'; | ||
+ | } | ||
+ | // close showed layer | ||
+ | function mclose() | ||
+ | { | ||
+ | if(ddmenuitem) ddmenuitem.style.visibility = 'hidden'; | ||
+ | } | ||
- | + | // go close timer | |
- | + | function mclosetime() | |
+ | { | ||
+ | closetimer = window.setTimeout(mclose, timeout); | ||
+ | } | ||
- | + | // cancel close timer | |
- | + | function mcancelclosetime() | |
+ | { | ||
+ | if(closetimer) | ||
+ | { | ||
+ | window.clearTimeout(closetimer); | ||
+ | closetimer = null; | ||
+ | } | ||
+ | } | ||
- | + | // close layer when click-out | |
- | < | + | document.onclick = mclose; |
+ | </script> | ||
+ | <style> | ||
+ | img.center { | ||
+ | display: block; | ||
+ | margin-left: auto; | ||
+ | margin-right: auto; | ||
+ | } | ||
+ | </style> | ||
+ | <!--main content --> | ||
+ | <table width="85%" align="center" bgcolor="#E6E6FA"> | ||
- | <td | + | <!--navigation menu --> |
- | + | <br> | |
+ | <td align="center" colspan="3"> | ||
- | < | + | <table width="100%" id="sddm"> |
- | < | + | <tr heigth="15px"></tr> |
+ | <tr heigth="75px"> | ||
- | <td style="border:1px | + | <div id="banner"><img id="idSelector" src="https://static.igem.org/mediawiki/2014/f/fa/Bannerugafinal.png" width="100%" height="100" align="center"></div> |
- | <a href="https://2014.igem.org/Team:UGA-Georgia | + | <td style="border:1px #fff;" align="center" height ="45px" width="250" onMouseOver="this.bgColor='#FF000'" onMouseOut="this.bgColor='#FEE5AD'" bgColor=#FEE5AD> |
+ | <a href="https://2014.igem.org/Team:UGA-Georgia"><p style="font-family: Basic L"><font size="2">HOME</font></p> </a> </td> | ||
- | <td style="border:1px | + | <td style="border:1px #fff" align="center" height ="45px" width="250" onMouseOver="this.bgColor='#FF000'" onMouseOut="this.bgColor='#FEE5AD'" bgColor=#FEE5AD> |
- | <a href="https://2014.igem.org/Team:UGA-Georgia/ | + | <a href="#" onmouseover="mopen('m1')" |
+ | onmouseout="mclosetime()" style="color:#000000" onMouseOver="this.bgColor='#FF000'" onMouseOut="this.bgColor='#FEE5AD'" bgColor=#FEE5AD><p style="font-family: Basic L"><font size="2"><b>PROJECT</b></font></p></a> | ||
+ | <div id="m1" | ||
+ | onmouseover="mcancelclosetime()" | ||
+ | onmouseout="mclosetime()"> | ||
+ | <a href=“https://2014.igem.org/Team:UGA-Georgia/Overview">Overview</a> | ||
+ | <a href="https://2014.igem.org/Team:UGA-Georgia/Geraniol">Geraniol</a> | ||
+ | <a href="https://2014.igem.org/Team:UGA-Georgia/Modeling">Modeling</a> | ||
+ | <a href="https://2014.igem.org/Team:UGA-Georgia/RBS">RBS Library</a> | ||
+ | <a href="https://2014.igem.org/Team:UGA-Georgia/Parts">Parts</a> | ||
+ | </div></td> | ||
+ | <td style="border:1px #fff;" align="center" height ="45px" width="250" onMouseOver="this.bgColor='#FF000'" onMouseOut="this.bgColor='#FEE5AD'" bgColor=#FEE5AD> | ||
+ | <a href="#" onmouseover="mopen('m3')" | ||
+ | onmouseout="mclosetime()" style="color:#000000"><p style="font-family: Basic L"><font size="2"><b>WET LAB</b></font></p></a> | ||
+ | <div id="m3" | ||
+ | onmouseover="mcancelclosetime()" | ||
+ | onmouseout="mclosetime()"> | ||
+ | <a href="https://2014.igem.org/Team:UGA-Georgia/Safety">Safety</a> | ||
+ | <a href="https://2014.igem.org/Team:UGA-Georgia/Notebook">Notebook</a> | ||
+ | <a href="https://2014.igem.org/Team:UGA-Georgia/Protocols">Protocols</a> | ||
+ | </div></td> | ||
+ | |||
+ | <td style="border:1px #fff;" align="center" height ="45px" width="250" onMouseOver="this.bgColor='#FF000'" onMouseOut="this.bgColor='#FEE5AD'" bgColor=#FEE5AD> | ||
+ | <a href="#" onmouseover="mopen('m4')" | ||
+ | onmouseout="mclosetime()" style="color:#000000"><p style="font-family: Basic L"><font size="2"><b>HUMAN PRACTICES</b></font></p></a> | ||
+ | <div id="m4" | ||
+ | onmouseover="mcancelclosetime()" | ||
+ | onmouseout="mclosetime()"> | ||
+ | <a href="https://2014.igem.org/Team:UGA-Georgia/Outreach">Outreach</a> | ||
+ | <a href="https://2014.igem.org/Team:UGA-Georgia/Seminars">Seminars</a> | ||
+ | </div></td> | ||
+ | |||
+ | <td style="border:1px #fff;" align="center" height ="45px" width="250" onMouseOver="this.bgColor='#FF000'" onMouseOut="this.bgColor='#FEE5AD'" bgColor=#FEE5AD> | ||
+ | <a href="#" onmouseover="mopen('m2')" | ||
+ | onmouseout="mclosetime()" style="color:#000000"><p style="font-family: Basic L"><font size="2"><b>TEAM</b></font></p></a> | ||
+ | <div id="m2" | ||
+ | onmouseover="mcancelclosetime()" | ||
+ | onmouseout="mclosetime()"> | ||
+ | <a href="https://igem.org/Team.cgi?id=1383">Official Team Profile</a> | ||
+ | <a href="https://2014.igem.org/Team:UGA-Georgia/Team">Members</a> | ||
+ | <a href="https://2014.igem.org/Team:UGA-Georgia/Attributions">Attributions</a> | ||
+ | |||
+ | </div></td> | ||
<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/2014/d/d2/UGA-iGEM_Logo.jpg" width="55px"></a> </td> | <td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/2014/d/d2/UGA-iGEM_Logo.jpg" width="55px"></a> </td> | ||
Line 66: | Line 185: | ||
</table> | </table> | ||
- | + | <!--end navigation menu --> | |
</tr> | </tr> | ||
- | |||
- | |||
</tr> | </tr> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
</td> | </td> | ||
Line 81: | Line 193: | ||
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr> | <tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr> | ||
<tr> <td colspan="3" height="5px"> </td></tr> | <tr> <td colspan="3" height="5px"> </td></tr> | ||
- | |||
<!--requirements section --> | <!--requirements section --> | ||
- | <tr><td colspan="3"> <h3>Notebook</h3></td></tr> | + | <tr><td colspan="3"> <h3><font size="6">Notebook</font></h3></td></tr> |
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td width=" | + | <td width="100%" valign="top"> |
- | <p> | + | <p><h4><font size="5"> February </font></h4> </p> |
+ | <br> | ||
+ | <h5> Week 1 </h5> | ||
+ | <li> Introduction to anaerobic facilities for new members </li> | ||
+ | <br> | ||
+ | <h5> Week 3 </h5> | ||
+ | <li> Making of NaS solution. </li> | ||
+ | <li> Making of formate media. </li> | ||
+ | <br> | ||
+ | <h5> Week 4 </h5> | ||
+ | <li> Creation of transformation buffer (TB). </li> | ||
+ | <li> First geraniol extraction efficiency test with balch tubes. </li> | ||
+ | <br> | ||
+ | <br> | ||
+ | <p><h4><font size="5"> March </font></h4></p> | ||
+ | <br> | ||
+ | <h5> Week 1 </h5> | ||
+ | <li> Creation of geraniol standards for gas chromatography (GC) analysis/calibration through serial dilutions. </li> | ||
+ | <br> | ||
+ | <h5> Week 3 </h5> | ||
+ | <li> Creation of more formate media. </li> | ||
+ | <li> Creation of general salts solution. </li> | ||
+ | <li> Creation of glycylglycine buffer. </li> | ||
+ | <ul> | ||
+ | <li> All of these solution protocols can be found on our <a href="https://2014.igem.org/Team:UGA-Georgia/Protocols">protocol</a> page. </li> | ||
+ | </ul> | ||
+ | <br> | ||
+ | <br> | ||
+ | <p><h4><font size="5"> April </font></h4></p> | ||
+ | <br> | ||
+ | <h5> Week 1</h5> | ||
+ | <li> Learning about ribosome binding site library (RBS) and our metabolic Optflux model. </li> | ||
+ | <br> | ||
+ | <h5> Week 2</h5> | ||
+ | <li> Made agar plates</li> | ||
+ | <li> Creation of fresh wild type (WT) stocks. </li> | ||
+ | <br> | ||
+ | <h5> Week 3 </h5> | ||
+ | <li> Created diluted puromycin </li> | ||
+ | <br> | ||
+ | <br> | ||
+ | <p><h4><font size="5"> May </font></h4></p> | ||
+ | <br> | ||
+ | <h5> Week 4 </h5> | ||
+ | <li> Inoculation and revival of frozen stocks. </li> | ||
+ | <br> | ||
+ | <br> | ||
+ | <p><h4><font size="5"> June </font></h4></p> | ||
+ | <br> | ||
+ | <h5> Week 1</h5> | ||
+ | <li> Determined the optical density (OD) of the previously revived cultures. </li> | ||
+ | |||
+ | |||
+ | <br> | ||
+ | <h5> Week 2 </h5> | ||
+ | <li> Restriction digestion, purification, and ligation of pMEV4 and PCR products (C1 and C2).</li> | ||
+ | <li> Transformation by heat shock, plated the cells, made an agarose gel and ran the products on it. </li> | ||
+ | <li> Picked colonies from the previous day of plating. </li> | ||
+ | <li> Plasmid extraction, digestion, gel electrophoresis, and verification. </li> | ||
+ | <li>[E. Coli Lab] Extracted pMEV4 from bacteria.</li> | ||
+ | <li>[E.Coli Lab] PRC for mCherry with 11 mutated RBS sites and 1 native RBS.</li> | ||
+ | <li>[E. Coli Lab] Vector pMEV4 was digested with Spe1, Pst1 and buffer 2. Gel Extraction, Ligation and Heat Shock Transformation were also performed.</li> | ||
+ | <li>[E. Coli Lab] Colonies were successfully formed from 1,3,4,8, and 11; PCR was redone for 2,5,6,7,10, and 12.</li> | ||
+ | <li>[E. Coli Lab] Created new LB plates with amplicillin and LB Broth.</li> | ||
+ | <li>[E. Coli Lab] Prepared competent cells for transformation</li> | ||
+ | <li>[E. Coli Lab] Obtained PCR product samples 2,5,6,7,10 and 12, and redid vector digestion, gel extraction, ligation, and heat shock transformation.</li> | ||
+ | <li>[E. Coli Lab] Sample 5,6,7 and 10 were unsuccessfully transformed. Redid vector digestion, gel extraction, ligation, and heat shock transformation.</li> | ||
+ | |||
+ | |||
+ | |||
+ | <br> | ||
+ | <h5> Week 3</h5> | ||
+ | <li> Revival of pAW50-mCherry frozen stock. </li> | ||
+ | <li> Revival of S0001 (WT) frozen stock. </li> | ||
+ | <li>[E. Coli Lab] Inoculation, plasmid extraction, digestion and screening were performed on 1, 3,4,5,8,9,10,11 and 12. </li> | ||
+ | <li>[E. Coli Lab] Screening <a href= "https://static.igem.org/mediawiki/2014/7/7f/Screening1-12.png">Part I</a>,<a href="https://2014.igem.org/File:Part2screening.png"> Part II</a> for 1,3,4,5,8,9,10,11 and 12. Verification was done by using KpnI and NcoI ( if positive:2750 and 2414; if negative: 2414 and 1986; control: pMEV4). </li> | ||
+ | <li>[E. Coli Lab] Cloning for 2,6, and 7 in progress</li> | ||
+ | <br> | ||
+ | <h5> Week 4 </h5> | ||
+ | <li> Prepared the revived cultures for fluorescence microscopy. </li> | ||
+ | <li> Fluorescence microscopy training. </li> | ||
+ | <li> Anaerobic transformation of RBS libraries 1-12 into <i> M. maripaludis. </i></li> | ||
+ | <li> Puromycin enrichment of transformants. </li> | ||
+ | <li> Made glycerol stocks of original transformants. </li> | ||
+ | <li> Fluorescent microscopy of transformants/pAW50-mCherry/S0001. </li> | ||
+ | <li> [E. Coli Lab] Plasmid extraction was done for 1,3,4,5,8,9,10,11 and 12. Also made 2 permanent stocks for each of them.</li> | ||
+ | <li> [E. Coli Lab] Plasmid extraction, inoculation, and screening were performed on 2, 6 and 7. Also made 2 permanent stocks for each of them</li> | ||
+ | <br> | ||
+ | <br> | ||
+ | <p><h4><font size="5"> July </font></h4></p> | ||
+ | <br> | ||
+ | <h5> Week 1 </h5> | ||
+ | <li> Took ODs of the native RBS and library 1 and 2 (12; L-1; L-2 respectively). </li> | ||
+ | <li> Plated the 12, L-1, and L-2 cultures. </li> | ||
+ | <li> Dispensed media to test tubes. </li> | ||
+ | <li> Picked colonies form the plates. </li> | ||
+ | <br> | ||
+ | <h5> Week 2 </h5> | ||
+ | <li> ODs from last weeks picked colonies were taken. </li> | ||
+ | <li> Made frozen stocks of RBS colonies, </li> | ||
+ | <li> Sub-cultured parent strains. </li> | ||
+ | <li> Researched primary literature on mCherry maturation. </li> | ||
+ | <li> Began developing a protocol for oxygen maturation of mCherry. </li> | ||
+ | <br> | ||
+ | <h5> Week 3 </h5> | ||
+ | <li> Made frozen stocks of RBS colonies and sub-cultured parent strains. </li> | ||
+ | <li> Re-subcultured and revive pAW50-mcherry. </li> | ||
+ | <li> Subcultured the parent strains of WT and native RBS (12) into triplicates. The triplicates were wrapped in foil to prevent any photobleaching. </li> | ||
+ | <li> The pAW50-mcherry came up and then all samples were taken to a plate reader to perform fluorescence tests. </li> | ||
+ | <li> [E. Coli Lab] the primers for the negative and positive control group were received; ran PCR on the negative and control groups in order to create a large amount of the DNA.</li> | ||
+ | <li> [E. Coli Lab] PCR amplification for mCherry gene with the negative and positive control.</li> | ||
+ | <li> [E. Coli Lab] Solid media was made.</li> | ||
+ | <li> [E. Coli Lab] <a href="https://static.igem.org/mediawiki/2014/a/a4/13-15PCRgel.png">PCR</a> was performed on 13, 14 and 15; vector digestion was also performed with Spe1, Pst1 and buffer2. </li> | ||
+ | <li> [E. Coli Lab] Gel electrophoresis was done to confirm <a href="https://static.igem.org/mediawiki/2014/4/4b/13-15Diggel.png">digestion </a>of 13-15. </li> | ||
+ | <br> | ||
+ | <h5> Week 5 </h5> | ||
+ | <li> Filled out and submitted safety form. </li> | ||
+ | <li> The geraniol extraction efficiency experiment was performed. </li> | ||
+ | <ul> | ||
+ | <li> Left the organic phase to dry over night and be resuspended to test for voltility. </li> | ||
+ | </ul> | ||
+ | <li> Track selection. </li> | ||
+ | |||
+ | <br> | ||
+ | <br> | ||
+ | <p><h4><font size="5"> August </font></h4></p> | ||
+ | <br> | ||
+ | <h5> Week 2</h5> | ||
+ | <li> Geraniol synthase containing cells (GS) were inoculated into triplicates of varying concentrations of puromycin. </li> | ||
+ | <ul> | ||
+ | <li> The previous geraniol extraction methods were repeated with these new GS samples. </li> | ||
+ | </ul> | ||
+ | <br> | ||
+ | <h5> Week 3</h5> | ||
+ | <li> Geraniol standards were made to be test on GC/MS. </li> | ||
+ | <li> We did an extraction efficiency test between batch tube vs. separatory funnel. </li> | ||
+ | <li> Testing of geraniol on the GC/MS was done. </li> | ||
+ | <br> | ||
+ | <h5> Week 4 </h5> | ||
+ | <li> Cells containing our pMEV4 plasmid with native RBS were inoculated into 5mL of rezasurin free (RF) media with varying levels of puromycin concentration.</li> | ||
+ | <li> ODs were taken of the samples and they were placed into darkness. </li> | ||
+ | <br> | ||
+ | <h5> Week 5</h5> | ||
+ | <li> The cells were prepared for the plate reader as per the first plate reader preparation protocol. </li> | ||
+ | <li> The plate reader results were in conclusion and a new protocol was required. </li> | ||
+ | <br> | ||
+ | <br> | ||
+ | <p><h4><font size="5"> September </font></h4></p> | ||
+ | <br> | ||
+ | <h5> Week 2 </h5> | ||
+ | <li> A frozen stock of pAW50-mCherry was revived in 5mL of RF media. </li> | ||
+ | <li> Room temperature stocks of 3 colonies of 12 were inoculated into 5mL of RF media. </li> | ||
+ | <ul> | ||
+ | <li> All of these will act as parent strains that will then be inoculated and grown in varying conditions to test for the best condition for generating mCherry. </li> | ||
+ | </ul> | ||
+ | <br> | ||
+ | <h5> Week 3</h5> | ||
+ | <li> The parent strains were inoculated and grown in the varying conditions. </li> | ||
+ | <ul> | ||
+ | <li> 37C vs. 30C </li> | ||
+ | <li> 100mL vs. 5mL media </li> | ||
+ | </ul> | ||
+ | <li> Some of each of the parent strains were also PCR tested to confirm the presence of the mCherry gene. </li> | ||
+ | <ul> | ||
+ | <li> The PCR showed that the mCherry gene was indeed present. </li> | ||
+ | </ul> | ||
+ | <br> | ||
+ | <h5> Week 5 </h5> | ||
+ | <li> ODs of the pAW50-mCherry and the 3 colonies of 12 that were grown in varying conditions were taken and they were all above OD=0.6. </li> | ||
+ | <li> The samples were prepared for overnight oxygen exposure as per the protocol. </li> | ||
+ | <li> The samples were taken to the plate reader to measure fluorescence. <li> | ||
+ | <ul> | ||
+ | <li> Fluorescence was measured for all the 12 samples in each condition. </li> | ||
+ | </ul> | ||
+ | <li> Since this pre-study gave us consistent, positive values for fluorescence, we were able to now refine the oxygen exposure protocol and apply it to characterization of our parts. </li> | ||
+ | <ul> | ||
+ | <li> This detailed protocol can be found in our <a href="https://2014.igem.org/Team:UGA-Georgia/Protocols">protocol</a> section. </li> | ||
+ | </ul> | ||
+ | <li> Revival of frozen stocks of cells containing plasmids with our theoretical "perfect" RBS (14), cells containing our theoretical "negative" RBS (15), and each of the 3 colonies of 12. Also, revival of WT with no plasmid were revived from a room temperature stock (negative control). </li> | ||
+ | <li> [E. Coli Lab] PCR Verification of 12C1, 12C2 and 12C3 was done with F12 as forward primer and R as reverse primer.</li> | ||
+ | <br> | ||
+ | <br> | ||
+ | <p><h4><font size="5">October</font></h4></p> | ||
+ | <br> | ||
+ | <h5> Week 1</h5> | ||
+ | <li> The revived parent strains of 14, 15, WT, and 12 C-1,2,3 were inoculated into varying volumes, and in triplicates, to test for a linear relationship of mCherry production to volume of culture which would be determined after the samples were taken to the plate reader. </li> | ||
+ | <ul> | ||
+ | <li> The triplicates were grown in 5mL, 25mL, and one single sample each of 12 C-1, 14, and 15 were grown into 100mL as a reference. </li> | ||
+ | </ul> | ||
+ | <li> [E. Coli Lab] Cloning for BioBrick (Genes: BBa_K1383000{Native RBS; F12 with F12 and R primers}, BBa_K1383001{Theoretical Best RBS; F14 with F14 and R primers}, and BBa_K1383002{Theoretical Worst RBS; F15 with F15 and R primers}; Vector: pSB1C3); PCR <a href="https://static.igem.org/mediawiki/2014/0/08/Biobrickpcr.png">result </a> was observed by using gel electrophoresis and UV light. </li> | ||
+ | <li> [E. Coli Lab] Plasmid extraction, purification, and <a href="https://static.igem.org/mediawiki/2014/a/a4/Biobrickscreening10-03-2014.JPG"> screening </a> were performed on F12A, F12B, F12C, F14A, F14B, F14C, F15A, F15B, and F15C. </li> | ||
+ | <br> | ||
+ | <h5> Week 2</h5> | ||
+ | <li> The oxygen exposure <a href="https://2014.igem.org/Team:UGA-Georgia/Protocols">protocol</a> was performed on the triplicates and the results of the experiment can be found on our <a href="https://2014.igem.org/Team:UGA-Georgia/RBS">RBS Library</a> page. </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
</td> | </td> | ||
+ | |||
<td></td> | <td></td> | ||
Line 99: | Line 408: | ||
</table> | </table> | ||
+ | </body> | ||
</html> | </html> |
Latest revision as of 02:30, 18 October 2014
|
|||||
Notebook | |||||
FebruaryWeek 1Week 3Week 4MarchWeek 1Week 3
AprilWeek 1Week 2Week 3MayWeek 4JuneWeek 1Week 2Week 3Week 4JulyWeek 1Week 2Week 3Week 5
AugustWeek 2
Week 3Week 4Week 5SeptemberWeek 2
Week 3
Week 5
OctoberWeek 1
Week 2 |