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Team:Bordeaux/NotebookJuly16
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Latest revision as of 02:25, 18 October 2014
16th July
Molecular biology
- SLP gene synthesis
PCR Parameter:
98°C 5min
35 cycles : 98°C 10s, Gradient 68°C delta4°C : (2AB,2AB°,2AB°°,2AB°°°) (3) 67°C 20s ; (2CD,2CD°,2CD°°,2CD°°°) (10) 69°C 20s, 72°C 1min
Final extension 72°C 10min
End at 4°C
We obtain this gel
PCR Parameter:
98°C 5min
35 cycles : 98°C 10s, Gradient 63°C delta14°C : (2AB) (6) 66°C 20s ; (2CD) (12) 69°C 20s ; (3 (45)) (9) 66°C 20s ; (3 (primer1,8)) (9) 66°C 20s ; (3(45)(primer1,8)) (9) 66°C 20s, 72°C 1 min
Final extension 72°C 10min
End at 4°C
Production and purification
Please look our planning of production and purification
