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Team:British Columbia/Results
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Before attempting to demonstrate binding with peptide inserted into the S-Layer, we tested binding with WT cells. After having observed binding, we then hypothesized that the fucose layer in Caulobacter was potentially involved in binding. Since there was a fucos knockout was available, we ere able to confirm fucos binding. Moreover, this observation demonstrated that a precipitation assay was feasible when mixing cells with chalcopyrite. | Before attempting to demonstrate binding with peptide inserted into the S-Layer, we tested binding with WT cells. After having observed binding, we then hypothesized that the fucose layer in Caulobacter was potentially involved in binding. Since there was a fucos knockout was available, we ere able to confirm fucos binding. Moreover, this observation demonstrated that a precipitation assay was feasible when mixing cells with chalcopyrite. | ||
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'''Figure 2.''' Various peptides from Curtis ''et al.'' are displayed in different positions in the S-layer of ''Caulobacter'' cells. The cells were mixed with calcopyrite and precipitate settling was observed. | '''Figure 2.''' Various peptides from Curtis ''et al.'' are displayed in different positions in the S-layer of ''Caulobacter'' cells. The cells were mixed with calcopyrite and precipitate settling was observed. | ||
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Revision as of 02:24, 18 October 2014
Results
Biomining precipitation assays
Before attempting to demonstrate binding with peptide inserted into the S-Layer, we tested binding with WT cells. After having observed binding, we then hypothesized that the fucose layer in Caulobacter was potentially involved in binding. Since there was a fucos knockout was available, we ere able to confirm fucos binding. Moreover, this observation demonstrated that a precipitation assay was feasible when mixing cells with chalcopyrite.
Figure 1. Wild type and fucos knockout Caulobacter cells mixed with Chalcopyrite.
As a preliminary test, we observed increased Chalcopyrite precipitation with Caulobacter cells expressing a Chalcopyrite binding peptide in the S-layer. While non-quantitative, the data showed noticeable differences in precipitate settle when the peptide expressed in various locations in the S-layer. See the video below.
Figure 2. Various peptides from Curtis et al. are displayed in different positions in the S-layer of Caulobacter cells. The cells were mixed with calcopyrite and precipitate settling was observed.
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