Team:Aberdeen Scotland/Modeling

From 2014.igem.org

(Difference between revisions)
 
(6 intermediate revisions not shown)
Line 12: Line 12:
<!-- Include CSS -->
<!-- Include CSS -->
<link rel="stylesheet" type="text/css" href="https://2014.igem.org/Template:Team:Aberdeen_Scotland/CSS?action=raw&ctype=text/css">
<link rel="stylesheet" type="text/css" href="https://2014.igem.org/Template:Team:Aberdeen_Scotland/CSS?action=raw&ctype=text/css">
 +
 +
<!-- Include MathJax -->
 +
<script type="text/javascript" src="http://cdn.mathjax.org/mathjax/latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script>
</head>
</head>
Line 30: Line 33:
<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Safety">Safety</a></li>
<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Safety">Safety</a></li>
<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Attributions">Attributions</a></li>
<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Attributions">Attributions</a></li>
 +
<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Ethics">Ethics & Outreach</a></li>
</ul>
</ul>
<div id="social">
<div id="social">
Line 47: Line 51:
<div id="sidebar">
<div id="sidebar">
<ul class="sidebar">
<ul class="sidebar">
-
<li class="curr"><a class="curr" href="#">Blank</a></li>
+
<li class="curr"><a class="curr" href="https://2014.igem.org/Team:Aberdeen_Scotland/Modeling">Introduction</a></li>
-
<li><a href="#">Blank</a></li>
+
<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Modeling/QS">Quorum Sensing</a></li>
-
<li><a href="#">Blank</a></li>
+
<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Modeling/GFP">GFP Response</a></li>
 +
<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Modeling/Assay">Assay Sensitivity</a></li>
</ul>
</ul>
</div>
</div>
Line 58: Line 63:
<!-- SECTION HEAD -->
<!-- SECTION HEAD -->
<div class="t_overview">
<div class="t_overview">
-
<h1>Overview</h1>
+
<h1>Modelling Aims</h1>
-
<p>We are a team of undergrads studying in University of Aberdeen. There's six of us - 5 biologists and 1 physicist.</p>
+
<!-- <h3></h3> -->
</div> <br class="clear"> <!-- END OF HEAD -->
</div> <br class="clear"> <!-- END OF HEAD -->
-
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod
+
-
tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam,
+
<!-- PAGE CONTENT -->
-
quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo
+
<div class="main_content">
-
consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse
+
<h3>Purpose</h3>
-
cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non
+
<p></p>
-
proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
<p>In order to verify and characterize our diagnostic system we created mathematical models for each individual component. Modeling and simulations were essential
 +
techniques that guided our approach from design to completion. As the main point of interest in our system was inter-cellular communication and its optimization, we
 +
employed ODEs and PDEs as our main mathematical tools.</p>
 +
<img src="https://static.igem.org/mediawiki/2014/a/a7/AjkIkdjfmkJ.png">
 +
<h3>Quorum Sensing</h3>
 +
<p>We developed a spacial model for analyzing Quorum Sensing between cells and then studied it under our system desired conditions. This gave us insight into how
 +
best structure our assay.</p>
 +
<h3>GFP Response</h3>
 +
<p>By simulating GFP response we made sure our system will react in a predictable manner and in an appropriate amount of time. We ensured that simulations agreed with
 +
data so that we can rely on reproducibility.</p>
 +
<h3>Assay Sensitivity</h3>
 +
<p>As with any other test, sensitivity is a main factor. The test needed to be sensitive enough to detect HAT, but also tolerant to noise, so that false-positives
 +
were minimized. We explored the process of antibody binding to make sure we meet those criteria.</p>
 +
</div> <br class="clear"> <!-- END OF PAGE CONTENT -->
</div> <!-- END OF CONTAINER -->
</div> <!-- END OF CONTAINER -->

Latest revision as of 02:20, 18 October 2014

Team:Aberdeen Scotland/Modelling - 2014.ogem.org



Modelling Aims


Purpose

In order to verify and characterize our diagnostic system we created mathematical models for each individual component. Modeling and simulations were essential techniques that guided our approach from design to completion. As the main point of interest in our system was inter-cellular communication and its optimization, we employed ODEs and PDEs as our main mathematical tools.

Quorum Sensing

We developed a spacial model for analyzing Quorum Sensing between cells and then studied it under our system desired conditions. This gave us insight into how best structure our assay.

GFP Response

By simulating GFP response we made sure our system will react in a predictable manner and in an appropriate amount of time. We ensured that simulations agreed with data so that we can rely on reproducibility.

Assay Sensitivity

As with any other test, sensitivity is a main factor. The test needed to be sensitive enough to detect HAT, but also tolerant to noise, so that false-positives were minimized. We explored the process of antibody binding to make sure we meet those criteria.



Retrieved from "http://2014.igem.org/Team:Aberdeen_Scotland/Modeling"