Team:UGA-Georgia

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<h1 >WELCOME TO iGEM 2014! </h1>
 
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<p>Your team has been approved and you are ready to start the iGEM season!
 
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
 
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:UGA-Georgia&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
 
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<a href="https://igem.org/Team.cgi?year=2014&team_name=UGA-Georgia"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:UGA-Georgia" ><p style="font-family: Basic L"><font size="2">HOME</font></p> </a> </td>
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        <a href="https://2014.igem.org/Team:UGA-Georgia/Overview">Overview</a>
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        <a href="https://2014.igem.org/Team:UGA-Georgia/Geraniol">Geraniol</a>
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        <a href="https://2014.igem.org/Team:UGA-Georgia/Modeling">Modeling</a>
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        <a href="https://2014.igem.org/Team:UGA-Georgia/RBS">RBS Library</a>
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        <a href="https://2014.igem.org/Team:UGA-Georgia/Safety">Safety</a>
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        <a href="https://2014.igem.org/Team:UGA-Georgia/Notebook">Notebook</a>
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        <a href="https://2014.igem.org/Team:UGA-Georgia/Protocols">Protocols</a>
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        <a href="https://2014.igem.org/Team:UGA-Georgia/Outreach">Outreach</a>
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        <a href="https://2014.igem.org/Team:UGA-Georgia/Seminars">Seminars</a>
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        <a href="https://igem.org/Team.cgi?id=1383">Official Team Profile</a>
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        <a href="https://2014.igem.org/Team:UGA-Georgia/Team">Members</a>
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        <a href="https://2014.igem.org/Team:UGA-Georgia/Attributions">Attributions</a>
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<p>The 2014 University of Georgia iGEM team focuses on <em>two main goals</em>: <br><br>First, we aim to make methanogens a more tangible host for synthetic biologists. To achieve this, we are creating a metabolic flux model for the lipid biosynthesis pathway in <i>M. maripaludis</i>. Our goal is to map the entire pathway, complete a genome-scale flux balance analysis, and develop strategies for overproduction of isoprenoid compounds. In addition, ribosome binding sites (RBS) are not well characterized in Archaea, so we are creating a library of RBS sequences and measuring their relative strength of fluorescence. A library of RBS will allow us the ability for variable levels of expression. Also, we are developing new methods for extracting, concentrating, measuring and quantifying organically soluble material, like geraniol, from <i>M. maripaludis</i> cultures.</p>
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<p>Second, we are demonstrating the utility of methanogens in synthetic biology through optimized geraniol production. Following up on the work from the 2013 iGEM season, we’ve continued targeting geraniol as a high value bio-chemical which may be produced from inexpensive substrates such as H2/CO2 or formate. Uses of Geraniol include potential biofuel, organic pesticide, tumor suppressor of breast, colon and prostate cancers, fragrances and flavorings. Last year, geraniol production in <i>Methanococcus maripaludis</i> was quantified as 0.2% of lipid dry weight. This year, UGA-iGEM seeks to address the overall geraniol yield through several projects which satisfy both of our main goals mentioned above.<br></p></tr>
 
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<p> Please be sure to keep these links, your audience will want to find your: </p>
 
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<li><a href="https://2014.igem.org/Team:UGA-Georgia">Home</a> </li>
 
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<li><a href="https://2014.igem.org/Team:UGA-Georgia/Team">Team</a> </li>
 
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=UGA-Georgia">Official Team Profile</a> </li>
 
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<li><a href="https://2014.igem.org/Team:UGA-Georgia/Project">Project</a> </li>
 
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<li><a href="https://2014.igem.org/Team:UGA-Georgia/Parts">Parts</a> </li>
 
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<li><a href="https://2014.igem.org/Team:UGA-Georgia/Modeling">Modeling</a> </li>
 
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<li><a href="https://2014.igem.org/Team:UGA-Georgia/Notebook">Notebook</a> </li>
 
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<li><a href="https://2014.igem.org/Team:UGA-Georgia/Safety">Safety</a> </li>
 
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<li><a href="https://2014.igem.org/Team:UGA-Georgia/Attributions">Attributions</a> </li>
 
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<p>There are a few wiki requirements teams must follow:</p>
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<h3>Welcome!  <p>The New Archaea-Type
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
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<p>Scroll down to find more about our project! </p>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<h3>Read about our projects!</h3>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<p>The 2014 University of Georgia iGEM team focuses on <em>two main goals</em>: <br><br>First, we aim to make methanogens a more tangible host for synthetic biologists. To achieve this, we are creating a metabolic flux model for the lipid biosynthesis pathway in <i>Methanococcus maripaludis</i>. Our goal is to map the entire pathway, complete a genome-scale flux balance analysis, and develop strategies for overproduction of isoprenoid compounds. In addition, ribosome binding sites (RBS) are not well characterized in Archaea, so we are creating a library of RBS sequences and measuring their relative strength of fluorescence. A library of RBS will allow us the ability for variable levels of expression. Also, we are developing new methods for extracting, concentrating, measuring and quantifying organically soluble material from <i>M. maripaludis</i> cultures.<p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
 
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<p>Second, we are demonstrating the utility of methanogens in synthetic biology by the optimized production of geraniol, an organically soluble material. Following up on the work from the 2013 iGEM season, we’ve continued targeting geraniol as a high value bio-chemical which may be produced from inexpensive substrates such as H2/CO2 or formate. Uses of Geraniol include potential biofuel, organic pesticide, tumor suppressor of breast, colon and prostate cancers, fragrances and flavorings. Last year, geraniol production in <i>M. maripaludis</i> was quantified as 0.2% of lipid dry weight. This year, UGA-iGEM seeks to address the overall geraniol yield through several projects which satisfy both of our main goals mentioned above.<p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
 
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Latest revision as of 02:19, 18 October 2014


HOME

PROJECT

WET LAB

HUMAN PRACTICES

TEAM

Welcome!

The New Archaea-Type

Scroll down to find more about our project!

Read about our projects!

The 2014 University of Georgia iGEM team focuses on two main goals:

First, we aim to make methanogens a more tangible host for synthetic biologists. To achieve this, we are creating a metabolic flux model for the lipid biosynthesis pathway in Methanococcus maripaludis. Our goal is to map the entire pathway, complete a genome-scale flux balance analysis, and develop strategies for overproduction of isoprenoid compounds. In addition, ribosome binding sites (RBS) are not well characterized in Archaea, so we are creating a library of RBS sequences and measuring their relative strength of fluorescence. A library of RBS will allow us the ability for variable levels of expression. Also, we are developing new methods for extracting, concentrating, measuring and quantifying organically soluble material from M. maripaludis cultures.

Second, we are demonstrating the utility of methanogens in synthetic biology by the optimized production of geraniol, an organically soluble material. Following up on the work from the 2013 iGEM season, we’ve continued targeting geraniol as a high value bio-chemical which may be produced from inexpensive substrates such as H2/CO2 or formate. Uses of Geraniol include potential biofuel, organic pesticide, tumor suppressor of breast, colon and prostate cancers, fragrances and flavorings. Last year, geraniol production in M. maripaludis was quantified as 0.2% of lipid dry weight. This year, UGA-iGEM seeks to address the overall geraniol yield through several projects which satisfy both of our main goals mentioned above.
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