Team:UMayor-Chile/Project

From 2014.igem.org

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:UMayor-Chile/Project&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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  <body>
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    <!--navigation menu -->
 +
    <table width="100%">
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      <tbody>
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        <tr heigth="15px"></tr>
 +
        <tr heigth="75px">
 +
          <td style="border:1px solid black;" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://2014.igem.org/Team:UMayor-Chile" style="color:#000000">Home</a>
 +
          </td>
 +
          <td style="border:1px solid black;" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://2014.igem.org/Team:UMayor-Chile/Team" style="color:#000000">Our Polis</a>
 +
          </td>
 +
          <td style="border:1px solid black;" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://igem.org/Team.cgi?year=2014&amp;team_name=UMayor-Chile" style="color:#000000">Official Team Profile</a>
 +
          </td>
 +
          <td style="border:1px solid black" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://2014.igem.org/Team:UMayor-Chile/Project" style="color:#000000">E. coli Troyhorse</a>
 +
          </td>
 +
          <td style="border:1px solid black;" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://2014.igem.org/Team:UMayor-Chile/Parts" style="color:#000000">The DNA weapons of our Troyhorse</a>
 +
          </td>
 +
          <td style="border:1px solid black;" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://2014.igem.org/Team:UMayor-Chile/Notebook" style="color:#000000">Out the lab</a>
 +
          </td>
 +
          <td style="border:1px solid black;" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://2014.igem.org/Team:UMayor-Chile/Safety" style=" color:#000000">Safety</a>
 +
          </td>
 +
          <td style="border:1px solid black;" align="center" height="45px" bgcolor="#e7e7e7" onmouseover="this.bgColor='#d3d3d3'" onmouseout="this.bgColor='#e7e7e7'">
 +
            <a href="https://2014.igem.org/Team:UMayor-Chile/Attributions" style="color:#000000">Attributions</a>
 +
          </td>
 +
          <td align="center">
 +
            <a href="https://2014.igem.org/Main_Page">
 +
              <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px">
 +
            </a>
 +
          </td>
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        </tr>
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      </tbody>
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    </table>
 +
    <!--end navigation menu -->
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    <div class="gwd-div-t8w1"><span class="gwd-span-suxd">The cystic fibrosis</span>
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      <div>
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        <br>
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<tr heigth="75px">  
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      </div>
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      <div>
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        <br>
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      </div>
 +
    </div>
 +
    <div class="gwd-div-zi30">
 +
      <div class="gwd-div-5ynf"><span class="gwd-span-gks4">Our
 +
troyhorse warriors have a plan!!</span>
 +
      </div>
 +
    </div>
 +
    <div class="gwd-div-16g8">
 +
      <div><span class="gwd-span-bby2">Our troyhorse warriors have a plan!!</span>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile"style="color:#000000">Home </a> </td>
+
      <div><span class="gwd-span-13sl"><br></span>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile/Team"style="color:#000000"> Team </a> </td>
+
      <div><span class="gwd-span-5rqd">We propose smart warriors E. coli for the battle against the P. aeruginosa. We are training them now, teaching the action plan!!! Pay attention...</span>
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<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
      </div>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=UMayor-Chile"style="color:#000000"> Official Team Profile </a></td>
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    </div>
 +
    <img src="https://static.igem.org/mediawiki/2014/a/a2/2cz8xw6.png" class="gwd-img-7ff3">
 +
    <div class="gwd-div-rsut">
 +
      <div><span class="gwd-span-8joy">http://redsalud.uc.cl/ucchristus/vidasaludable/glosario/F/fibrosis_quistica.act</span>
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<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile/Project"style="color:#000000"> Project</a></td>
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      <div><span class="gwd-span-hz9p"><br></span>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile/Parts"style="color:#000000"> Parts</a></td>
+
      <div><span class="gwd-span-sbih">http://www.fibrosisquisticadelpancreas.cl/noticias/10-generales/16-ministro-de-salud-inauguro-congreso-de-actualizacion-en-fibrosis-quistica-.html</span>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile/Modeling"style="color:#000000"> Modeling</a></td>
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      <div><span class="gwd-span-1bo4"><br></span>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile/Notebook"style="color:#000000"> Out the lab</a></td>
+
      <div><span class="gwd-span-a3q8">http://www.scielo.cl/scielo.php?script=sci_arttext&amp;pid=S0370-41062014000300003</span>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile/Safety"style=" color:#000000"> Safety </a></td>
+
      <div><span class="gwd-span-07pf"><br></span>
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
      </div>
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<a href="https://2014.igem.org/Team:UMayor-Chile/Attributions"style="color:#000000"> Attributions </a></td>
+
      <div><span class="gwd-span-qet1">http://www.cff.org/</span>
 +
      </div>
 +
      <div><span class="gwd-span-t5ry"><br></span>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
+
      </div>
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</tr>
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      <div><span class="gwd-span-dk6w">Taylor Sitarik Cohen &amp; Alice Prince. Cystic fibrosis: a mucosal immunodeficiency syndrome (doi:10.1038/nm.2715)</span>
-
</table>
+
-
</tr>
+
      </div>
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</tr>
+
    </div>
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</td>
+
    <div class="gwd-div-k4ez"></div>
 +
    <div class="gwd-div-zoic">
 +
      <div><span class="gwd-span-djbn">Say goodbye everybody, the antibiotics are now available:</span>
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
      </div>
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<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+
      <div><span class="gwd-span-yg95"><br></span>
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<tr> <td colspan="3"  height="5px"> </td></tr>
+
 +
      </div>
 +
      <div><span class="gwd-span-znew">&nbsp;There’s no protection of the biofilm. Both P. aeruginosa and our E. coli Troyhorse are exposed to the final cut… a massive antibiotic attack!!!</span>
 +
      </div>
 +
      <div>
 +
        <br>
 +
      </div>
 +
    </div>
 +
    <div class="gwd-div-hk3q">
 +
      <div><span class="gwd-span-pwtb">&nbsp;The first part of the plan is simple: looking for the Biofilm fortress.</span>
-
<!--Project content  -->
+
      </div>
-
<tr><td > <h3> Cystic fibrosis (CF) </h3></td>
+
      <div><span class="gwd-span-wk45"><br></span>
-
<td > </td >
+
-
<td > <h3> Our troyhorse warriors have a plan!!!</h3></td>
+
-
</tr>
+
-
<tr>
+
      </div>
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<td width="45%"  valign="top">  
+
      <div><span class="gwd-span-v3j0">&nbsp; &nbsp;We have to find our enemy. Our warriors will to enter to the fortress been guide by the clues left by our enemies, the Pseudomonas aeruguinosa.</span>
-
<p>70,000 people with CF worldwide, a lifespan depending of the resources (in USA the lifespan it’s about 40 years old, while in Chile the average lifespan it’s about 18 years old).</p>
+
-
<br>
+
-
<p>It’s a disease caused by the expression of a deficient transmembrane protein called CFTR. This affects different kind of cells, mainly the epithelial cells from the airway and pancreas, causing the production of a defective mucus.</p>
+
-
<br>
+
-
<p> In the lungs, this defective mucus leads that several opportunistic pathogens enter to the lower airway and initiate an infection, with a consecutively progressive lung dysfunction.</p>
+
-
<br>
+
-
<p>One of the main pathogens found in an infection of the lungs are the Pseudomonas aeruginosa. When it colonize the airway, initiates the production of biofilm, a barrier that protect it of a lot of antimicrobial substances and gave best environment for it develop.</p>
+
-
<br>
+
-
<p>The actual treatments for controlling the symptoms consist in maintain controlled the pathogens, clear the airway of the sticky mucus, administrate complementary enzymes and regulate the function of the CFTR channel.</p>
+
-
<br>
+
-
<h3>References </h3>
+
-
<p>http://redsalud.uc.cl/ucchristus/vidasaludable/glosario/F/fibrosis_quistica.act</p>
+
-
<p>http://www.fibrosisquisticadelpancreas.cl/noticias/10-generales/16-ministro-de-salud-inauguro-congreso-de-actualizacion-en-fibrosis-quistica-.html</p>
+
-
<p>http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0370-41062014000300003</p>
+
-
<p>http://www.cff.org/</p>
+
-
<p>Taylor Sitarik Cohen & Alice Prince. Cystic fibrosis: a mucosal immunodeficiency syndrome (doi:10.1038/nm.2715)</p>
+
-
</td>
+
-
<td></td>
+
      </div>
-
<td  width="45%" valign="top">
+
    </div>
-
<p> We propose smart warriors E. coli for the battle against the P. aeruginosa. We are training them now, teaching the action plan!!! Pay attention:</p>
+
    <div class="gwd-div-ienk">
-
<br>
+
      <div><span class="gwd-span-pfyf">Well, here we are… it’s time to start the part II:</span>
 +
      </div>
 +
      <div><span class="gwd-span-ap0f"><br></span>
-
<p>
+
      </div>
-
1. The first part of the plan is simple: looking for the Biofilm fortress.</p>
+
      <div><span class="gwd-span-37e7">&nbsp; Our E. coli tryhorse now know they are in the zone. It’s time to start the attack. Our enemy are protected by his thick barrier and our warriors know his weak point.&nbsp;</span>
-
<p>We have to find our enemy. Our warriors will enter to the fortress been guide by the clues left by our enemies, the Pseudomonas aeruguinosa.</p>
+
      </div>
-
<br>
+
    </div>
 +
    <div class="gwd-div-orp2">
 +
      <div><span class="gwd-span-9fwt">They are under attack!!! Pseudomonas are communicating themselves for rebuild the biofilm:</span>
-
<p>2. Well, here we are… it’s time to start the part II:</p>
+
      </div>
 +
      <div><span class="gwd-span-zshs"><br></span>
-
<p>Our E. coli Troyhorse now know they are in the zone. It’s time to start the attack. Our enemy are protected by his thick barrier and our warriors know his weak point.</p>
+
      </div>
-
<br>
+
      <div><span class="gwd-span-06vs">&nbsp; We have to cut the communications between the Pseudomonas as an intelligent strategy. The biofilm it’s a community work. If they can´t send signals, they won’t work in community.</span>
-
<p>3. They are under attack!!! Pseudomonas are communicating themselves to rebuild the biofilm:</p>
+
      </div>
 +
    </div>
 +
    <div class="gwd-div-4inh">
 +
      <div class="gwd-div-86v0"><span class="gwd-span-v6y8">70,000 people with CF worldwide, a lifespan depending of the resources (in USA the lifespan it’s about 40 years old, while in Chile the average lifespan it’s about 18 years old)</span>
-
<p>
+
      </div>
-
We have to cut the communications between the Pseudomonas as an intelligent strategy. The biofilm it’s a community work. If they can´t send signals, they won’t work in community.</p>
+
    </div>
-
<br>
+
    <div class="gwd-div-doj2">
 +
      <div class="gwd-div-ay25"><span class="gwd-span-bwuo">Scene from Patient Assistance Resource Center video (Youtube). Cystic Fibrosis Foundation.</span>
-
<p>4. Say goodbye everybody, the antibiotics are now available:</p>
+
      </div>
 +
    </div>
 +
    <div class="gwd-div-gnb3">
 +
      <div><span class="gwd-span-5all">It’s a disease caused by the expression of a deficient transmembrane protein called CFTR. This affects different kind of cells, mainly the epithelial cells from the airway and pancreas, causing the production of a defective mucus.</span>
-
<p>
+
      </div>
-
There’s no protection of the biofilm. Both P. aeruginosa and our E. coli Troyhorse are exposed to the final cut… a massive antibiotic attack!!!</p>
+
      <div><span class="gwd-span-bnjd"><br></span>
-
<br>
+
-
</td>
+
      </div>
 +
      <div><span class="gwd-span-gxwd">&nbsp;In the lungs, this defective mucus leads that several opportunistic pathogens enter to the lower airway and initiate an infection, with a consecutively progressive lung dysfunction.</span>
-
</tr>
+
      </div>
 +
    </div>
 +
    <img src="https://static.igem.org/mediawiki/2014/e/e9/Imagen1eng.png" class="gwd-img-4tve">
 +
    <img src="https://static.igem.org/mediawiki/2014/8/87/Cap1212.jpg" class="gwd-img-ezf3">
 +
    <div class="gwd-div-txah">
 +
      <p class="MsoNormal"><span lang="EN-US" class="gwd-span-0jrz"><span class="gwd-span-1gxd">One of the
 +
main pathogens found in an infection of the lungs are the <em>Pseudomonas
 +
aeruginosa</em>. When it colonize the airway, initiates the production of biofilm, a
 +
barrier that protect it of a lot of antimicrobial substances and gave best environment
 +
for it develop.</span>
 +
        <o:p></o:p>
 +
        </span>
 +
      </p>
 +
      <p class="MsoNormal"><span lang="EN-US"><span class="gwd-span-6n79"><span class="gwd-span-wzif">&nbsp;The actual treatments for controlling the symptoms
 +
consist in maintain controlled the pathogens, clear the airway of the sticky
 +
mucus, administrate complementary enzymes and regulate the function of the CFTR
 +
channel.&nbsp;</span></span>
 +
        <o:p></o:p>
 +
        </span>
 +
      </p>
 +
    </div>
 +
    <img src="https://static.igem.org/mediawiki/2014/8/8d/Construct.png" class="gwd-img-llex">
 +
  </body>
-
</table>
 
</html>
</html>

Latest revision as of 02:19, 18 October 2014


Project

Home Our Polis Official Team Profile E. coli Troyhorse The DNA weapons of our Troyhorse Out the lab Safety Attributions
The cystic fibrosis


Our troyhorse warriors have a plan!!
Our troyhorse warriors have a plan!!

We propose smart warriors E. coli for the battle against the P. aeruginosa. We are training them now, teaching the action plan!!! Pay attention...
http://redsalud.uc.cl/ucchristus/vidasaludable/glosario/F/fibrosis_quistica.act

http://www.fibrosisquisticadelpancreas.cl/noticias/10-generales/16-ministro-de-salud-inauguro-congreso-de-actualizacion-en-fibrosis-quistica-.html

http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0370-41062014000300003

http://www.cff.org/

Taylor Sitarik Cohen & Alice Prince. Cystic fibrosis: a mucosal immunodeficiency syndrome (doi:10.1038/nm.2715)
Say goodbye everybody, the antibiotics are now available:

 There’s no protection of the biofilm. Both P. aeruginosa and our E. coli Troyhorse are exposed to the final cut… a massive antibiotic attack!!!

 The first part of the plan is simple: looking for the Biofilm fortress.

   We have to find our enemy. Our warriors will to enter to the fortress been guide by the clues left by our enemies, the Pseudomonas aeruguinosa.
Well, here we are… it’s time to start the part II:

  Our E. coli tryhorse now know they are in the zone. It’s time to start the attack. Our enemy are protected by his thick barrier and our warriors know his weak point. 
They are under attack!!! Pseudomonas are communicating themselves for rebuild the biofilm:

  We have to cut the communications between the Pseudomonas as an intelligent strategy. The biofilm it’s a community work. If they can´t send signals, they won’t work in community.
70,000 people with CF worldwide, a lifespan depending of the resources (in USA the lifespan it’s about 40 years old, while in Chile the average lifespan it’s about 18 years old)
Scene from Patient Assistance Resource Center video (Youtube). Cystic Fibrosis Foundation.
It’s a disease caused by the expression of a deficient transmembrane protein called CFTR. This affects different kind of cells, mainly the epithelial cells from the airway and pancreas, causing the production of a defective mucus.

 In the lungs, this defective mucus leads that several opportunistic pathogens enter to the lower airway and initiate an infection, with a consecutively progressive lung dysfunction.

One of the main pathogens found in an infection of the lungs are the Pseudomonas aeruginosa. When it colonize the airway, initiates the production of biofilm, a barrier that protect it of a lot of antimicrobial substances and gave best environment for it develop.

 The actual treatments for controlling the symptoms consist in maintain controlled the pathogens, clear the airway of the sticky mucus, administrate complementary enzymes and regulate the function of the CFTR channel. 

Retrieved from "http://2014.igem.org/Team:UMayor-Chile/Project"