Team:ITESM-CEM/Parts

From 2014.igem.org

(Difference between revisions)
 
(13 intermediate revisions not shown)
Line 49: Line 49:
   <tr>
   <tr>
     <td colspan="3" rowspan="3" align="left" valign="top"><ul>
     <td colspan="3" rowspan="3" align="left" valign="top"><ul>
-
       <sub>Submenú 1</sub><sub>Submenú 2</sub><sub>Submenú 3</sub>
+
       <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Parts" style="color: #FFF;">Our Parts</a></sub>
-
    </ul></td>
+
      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/List">List of our parts</a></sub>
 +
        </ul></td>
     <td><img src="images/spacer.gif" width="1" height="1" alt=""></td>
     <td><img src="images/spacer.gif" width="1" height="1" alt=""></td>
   </tr>
   </tr>
Line 64: Line 65:
<table width="100%" border="0" id="ContenidoSecciones">
<table width="100%" border="0" id="ContenidoSecciones">
<div style="background-color: #f3f3e2; style="width:95%">
<div style="background-color: #f3f3e2; style="width:95%">
 +
 +
<!--INICIO CONTENIDO-->
<!--INICIO CONTENIDO-->
 +
 +
 +
<h2>Our Parts</h2>
 +
 +
<iframe align="right" width="660" height="515" src="//www.youtube.com/embed/1mFwPDbb7UY" frameborder="0" allowfullscreen></iframe>
 +
 +
<p style="text-align: justify; text-justify: inter-word;"> The main goal of our project was to establish the construct which will help us to metabolize 7-ketocholesterol, consisting in the use of three specific enzymes, but for further applications we submitted them in single modules. This will serve as the basis of a future library for standardized work related to atherosclerosis.   
 +
</p><br>
 +
 +
<h2>Cholesterol Oxidase</h2>
 +
<p style="text-align: justify; text-justify: inter-word;"> This enzyme was first detected in <u>Chromobacterium sp.</u> We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1871 nucleotides and its codons were optimized in order to use it on <u>E. coli</u>, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.   
 +
</p> <br>
 +
 +
<h2>Oxoacyl Reductase</h2>
 +
<p style="text-align: justify; text-justify: inter-word;"> This enzyme was detected in <u>Rhodococcus jostii </u>. We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1007 nucleotides and its codons were optimized in order to use it on <u>E. coli</u>, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.
 +
</p> <br>
 +
 +
<h2>7-dehydratase</h2>
 +
<p style="text-align: justify; text-justify: inter-word;"> This enzyme (7-alpha dehydratase) was detected in <u>Rhodococcus jostii</u> . We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 602 nucleotides and its codons were optimized in order to use it on <u>E.coli</u>, it already included a stop codon for trancription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.
 +
</p> <br>
 +
 +
<h2>Neomycin Resistance</h2>
 +
<p style="text-align: justify; text-justify: inter-word;"> This selective marker was obtained from an mammalian expression vector. NeoR's length is 855 nucleotides and it was isolated from pcDNA3.1(-)/myc-His A.
 +
</p><br>
 +
 +
<h2>BGHPA</h2>
 +
<p style="text-align: justify; text-justify: inter-word;"> Bovine Growth Hormone Polyadenilation Signal for nuclease resistance. Translation terminator for eukaryotic cells.
 +
</p><br>
 +
 +
<h2>PCMV</h2>
 +
<p style="text-align: justify; text-justify: inter-word;"> Constitutive promoter from Cytomegalovirus, this promoter works on eukaryotic cells, driving protein expression.
 +
</p><br><br><br>
<br>
<br>
-
<table class="table table-striped">
+
 
-
<tr>
+
<!--FIN CONTENIDO-->
-
<th>Name</th>
+
 
-
<th>Type</th>
+
</div>
-
<th>Description</th>
+
</table>
-
<th>Designer</th>
+
      <p>&nbsp;</p></td>
-
<th>Length (bp)</th>
+
    <td><img src="images/spacer.gif" width="1" height="509" alt=""></td>
-
</tr>
+
  </tr>
-
<!-- Favorite-Name-Type-Description-Designer-Length-->
+
  <tr>
-
<tr>
+
    <td><img src="images/spacer.gif" width="240" height="1" alt=""></td>
-
<td><a href="http://parts.igem.org/Part:BBa_K1313000">BBa_K1313000</a></td>
+
    <td><img src="images/spacer.gif" width="544" height="1" alt=""></td>
-
<td>Enzyme</td>
+
    <td><img src="images/spacer.gif" width="133" height="1" alt=""></td>
-
<td>Colox</td>
+
    <td><img src="images/spacer.gif" width="70" height="1" alt=""></td>
-
<td>Eddie Cano Gámez</td>
+
    <td><img src="images/spacer.gif" width="165" height="1" alt=""></td>
-
<td>1</td>
+
    <td></td>
-
</tr>
+
  </tr>
-
<tr>
+
-
<td> <a href="http://parts.igem.org/Part:BBa_K1313001">BBa_K1313001</a></td>
+
-
<td>Enzyme</td>
+
-
<td>OxRed</td>
+
-
<td>Eddie Cano Gámez</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td> <a href="http://parts.igem.org/Part:BBa_K1313002"> BBa_K1313002 </a> </td>
+
-
<td>Enzyme</td>
+
-
<td>Dehydratase</td>
+
-
<td>Eddie Cano Gámez</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td> <a href="http://parts.igem.org/Part:BBa_K1313003"> BBa_K1313003 </a> </td>
+
-
<td>Antibiotic Resistance</td>
+
-
<td>NeoR</td>
+
-
<td>Carlos Meza Ramírez</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td> <a href="http://parts.igem.org/Part:BBa_K1313004"> BBa_K1313004 </a> </td>
+
-
<td>Promoter</td>
+
-
<td>pCMV/td>
+
-
<td>Oliva Sánchez Montesinos</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td> <a href="http://parts.igem.org/Part:BBa_K1313004"> BBa_K1313005 </a> </td>
+
-
<td>Stop</td>
+
-
<td>BGH PA/td>
+
-
<td>Oliva Sánchez Montesinos</td>
+
-
<td>1</td>
+
-
</tr>  
+
</table>
</table>
 +
 +
  <table width="100%" border="0" align="center" cellpadding="0" cellspacing="0" id="Table_" dwcopytype="CopyTableRow">
 +
    <tr>
 +
      <td colspan="5" align="left" valign="bottom">
 +
      <div class="footer">
 +
      <iframe src="https://2014.igem.org/Team:ITESM-CEM/FOOTER" width="500" height="82" frameborder="0" scrolling="no" seamless></iframe></div>
 +
      </td>
 +
      <td width="37%" align="right" valign="bottom">
        
        
-
</p>
+
      <div class="PLASMENU">
 +
      <iframe src="https://2014.igem.org/Team:ITESM-CEM/POPMENU" width="614" height="614" frameborder="0" scrolling="no" seamless></iframe>
 +
      </div>
 +
     
 +
      </td>
 +
    </tr>
 +
  </table>
 +
 
 +
</body>
 +
 
-
<!--FI
+
</html>

Latest revision as of 02:12, 18 October 2014

TEC-CEM | Parts

ITESM-CEM | Enzy7-K me

Parts 3256
 

Our Parts

The main goal of our project was to establish the construct which will help us to metabolize 7-ketocholesterol, consisting in the use of three specific enzymes, but for further applications we submitted them in single modules. This will serve as the basis of a future library for standardized work related to atherosclerosis.


Cholesterol Oxidase

This enzyme was first detected in Chromobacterium sp. We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1871 nucleotides and its codons were optimized in order to use it on E. coli, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.


Oxoacyl Reductase

This enzyme was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1007 nucleotides and its codons were optimized in order to use it on E. coli, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.


7-dehydratase

This enzyme (7-alpha dehydratase) was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 602 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon for trancription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin.


Neomycin Resistance

This selective marker was obtained from an mammalian expression vector. NeoR's length is 855 nucleotides and it was isolated from pcDNA3.1(-)/myc-His A.


BGHPA

Bovine Growth Hormone Polyadenilation Signal for nuclease resistance. Translation terminator for eukaryotic cells.


PCMV

Constitutive promoter from Cytomegalovirus, this promoter works on eukaryotic cells, driving protein expression.





 

Retrieved from "http://2014.igem.org/Team:ITESM-CEM/Parts"