Team:METU Turkey characterization
From 2014.igem.org
Ilkem.kumru (Talk | contribs) |
Ilkem.kumru (Talk | contribs) |
||
(4 intermediate revisions not shown) | |||
Line 88: | Line 88: | ||
<!-- Content goes here --> | <!-- Content goes here --> | ||
<h1> Characterization of K1392991</h1> | <h1> Characterization of K1392991</h1> | ||
- | |||
- | |||
+ | <h3> Here, we characterized the part K1392991 by ligasing K316003 and J23108. After centrifugation E. coli DH5a at 3000rpm for 3 minutes, we burst the cells and let the enzymes free in solution. Then, adding 1 mL of catechol(0.1M) into tube, color change into green. </h3> | ||
- | |||
+ | <p> At the following picture, there is Catechol (1,2-benzenediol) present in Left tube. It proves that in presence of catechol there is a degredation process in k316003+j23108. </h3> | ||
+ | <p> There is no Catechol (1,2-benzenediol) present in Right tube. It proves that in absence of catechol there is no degredation of PET in k316003+j23108. </p> | ||
- | < | + | |
+ | <p> This characterization contains parts that <a href="http://parts.igem.org/Part:BBa_K316003:Experience">Imperial_College_London</a> created in year 2010, we have proved that it works well and we aproved its function with our own construct of its use. </p> | ||
<img src="https://static.igem.org/mediawiki/2014/c/cf/Karakter.jpg"> | <img src="https://static.igem.org/mediawiki/2014/c/cf/Karakter.jpg"> | ||
- | <h3> | + | <h3> At the following picture, the liquid is 0.1M catechol (1,2 benzenediol) itself:</h3> |
<p> <img src="https://static.igem.org/mediawiki/2014/6/6d/Catecholkoyduk.jpg"> </p> | <p> <img src="https://static.igem.org/mediawiki/2014/6/6d/Catecholkoyduk.jpg"> </p> | ||
Line 133: | Line 134: | ||
<img src="https://static.igem.org/mediawiki/2014/e/e9/Asilcircularlin.png"> | <img src="https://static.igem.org/mediawiki/2014/e/e9/Asilcircularlin.png"> | ||
- | + | <p> According to gel image: Left, part is closed to 2300bp. Right, linearized backbone is closed to 4000bp. </p> | |
</td> | </td> | ||
Line 161: | Line 162: | ||
<!-- Content goes here --> | <!-- Content goes here --> | ||
- | <img src="https://static.igem.org/mediawiki/2014/ | + | <img src="https://static.igem.org/mediawiki/2014/e/e8/Hydratase.PNG"> |
- | <h3> | + | <h3> K1392931 (Hydratase, 810bp)</h3> |
- | <p> | + | <p> We got those photos after PCR of E. coli BL21 (DE3). We have tried colony and liquid culture of E coli. BL21 (DE3)</p> |
</td> | </td> | ||
Line 189: | Line 190: | ||
<!-- Content goes here --> | <!-- Content goes here --> | ||
- | <img src="https://static.igem.org/mediawiki/2014/ | + | <img src="https://static.igem.org/mediawiki/2014/1/1c/Aldolase.PNG"> |
- | <h3> | + | <h3>K1392932 (Aldolase, 1014bp)</h3> |
- | <p> | + | <p> We got those photos after PCR of E. coli BL21 (DE3). We have tried colony and liquid culture of E coli. BL21 (DE3) </p> |
</td> | </td> |
Latest revision as of 02:10, 18 October 2014
Team:METU Turkey/Templates/Navigationbar
HOME | TEAM | PROJECT | PARTS | MODELING | INTERLAB STUDY |
POLICY&PRACTICE | CHARACTERIZATION | SAFETY | ATTRIBUTIONS | GALLERY | PROTOCOLS |
![]() |
![]() |
|
|
|
|
![]() |
![]() |
![]() |
![]() |
![]() |
|
![]() |
|
![]() |
Our Supporter: | ||
|