Team:METU Turkey characterization
From 2014.igem.org
Ilkem.kumru (Talk | contribs) (Created page with "{{:Team:METU_Turkey/Templates/Navigationbar}} {{CSS/Main}} <html> <style> #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear...") |
Ilkem.kumru (Talk | contribs) |
||
(16 intermediate revisions not shown) | |||
Line 15: | Line 15: | ||
<table id="menu" width="100%" cellspacing="0" height="40px"> | <table id="menu" width="100%" cellspacing="0" height="40px"> | ||
<tr> | <tr> | ||
- | |||
- | |||
- | |||
<table align="center"> | <table align="center"> | ||
Line 35: | Line 32: | ||
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> | <td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> | ||
<a href="https://2014.igem.org/Team:METU_Turkey_modeling" style="text-decoration:none;color:#1C140D">MODELING </a> </td> | <a href="https://2014.igem.org/Team:METU_Turkey_modeling" style="text-decoration:none;color:#1C140D">MODELING </a> </td> | ||
- | + | ||
- | + | ||
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> | <td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> | ||
<a href="https://2014.igem.org/Team:METU_Turkey_interlabstudy" style="text-decoration:none;color:#1C140D">INTERLAB STUDY </a></td> | <a href="https://2014.igem.org/Team:METU_Turkey_interlabstudy" style="text-decoration:none;color:#1C140D">INTERLAB STUDY </a></td> | ||
+ | |||
+ | </tr> | ||
+ | <tr height="50px"> | ||
+ | |||
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> | <td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> | ||
Line 53: | Line 53: | ||
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> | <td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> | ||
<a href="https://2014.igem.org/Team:METU_Turkey_attributions" style="text-decoration:none;color:#1C140D">ATTRIBUTIONS </a></td> | <a href="https://2014.igem.org/Team:METU_Turkey_attributions" style="text-decoration:none;color:#1C140D">ATTRIBUTIONS </a></td> | ||
+ | |||
+ | <td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> | ||
+ | <a href="https://2014.igem.org/Team:METU_Turkey_gallery" style="text-decoration:none;color:#1C140D">GALLERY </a></td> | ||
+ | |||
+ | <td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2> | ||
+ | <a href="https://2014.igem.org/Team:METU_Turkey_protocols" style="text-decoration:none;color:#1C140D">PROTOCOLS </a></td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
</td> | </td> | ||
- | |||
</tr> | </tr> | ||
</table> | </table> | ||
Line 69: | Line 74: | ||
</table> | </table> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
<!-- Block of content 1 --> | <!-- Block of content 1 --> | ||
<table width="100%" cellspacing="0" height="900px"> | <table width="100%" cellspacing="0" height="900px"> | ||
Line 109: | Line 87: | ||
<!-- Content goes here --> | <!-- Content goes here --> | ||
- | < | + | <h1> Characterization of K1392991</h1> |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | < | + | <h3> Here, we characterized the part K1392991 by ligasing K316003 and J23108. After centrifugation E. coli DH5a at 3000rpm for 3 minutes, we burst the cells and let the enzymes free in solution. Then, adding 1 mL of catechol(0.1M) into tube, color change into green. </h3> |
- | + | ||
- | + | ||
- | <img src="https://static.igem.org/mediawiki/2014/ | + | |
+ | <p> At the following picture, there is Catechol (1,2-benzenediol) present in Left tube. It proves that in presence of catechol there is a degredation process in k316003+j23108. </h3> | ||
+ | <p> There is no Catechol (1,2-benzenediol) present in Right tube. It proves that in absence of catechol there is no degredation of PET in k316003+j23108. </p> | ||
+ | |||
+ | |||
+ | <p> This characterization contains parts that <a href="http://parts.igem.org/Part:BBa_K316003:Experience">Imperial_College_London</a> created in year 2010, we have proved that it works well and we aproved its function with our own construct of its use. </p> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/c/cf/Karakter.jpg"> | ||
+ | |||
+ | <h3> At the following picture, the liquid is 0.1M catechol (1,2 benzenediol) itself:</h3> | ||
+ | <p> <img src="https://static.igem.org/mediawiki/2014/6/6d/Catecholkoyduk.jpg"> </p> | ||
Line 154: | Line 132: | ||
<!-- Content goes here --> | <!-- Content goes here --> | ||
- | <img src="https://static.igem.org/mediawiki/2014/ | + | <img src="https://static.igem.org/mediawiki/2014/e/e9/Asilcircularlin.png"> |
- | + | ||
- | <p> | + | <p> According to gel image: Left, part is closed to 2300bp. Right, linearized backbone is closed to 4000bp. </p> |
</td> | </td> | ||
Line 184: | Line 162: | ||
<!-- Content goes here --> | <!-- Content goes here --> | ||
- | <img src="https://static.igem.org/mediawiki/2014/ | + | <img src="https://static.igem.org/mediawiki/2014/e/e8/Hydratase.PNG"> |
- | <h3> | + | <h3> K1392931 (Hydratase, 810bp)</h3> |
- | <p> | + | <p> We got those photos after PCR of E. coli BL21 (DE3). We have tried colony and liquid culture of E coli. BL21 (DE3)</p> |
</td> | </td> | ||
Line 212: | Line 190: | ||
<!-- Content goes here --> | <!-- Content goes here --> | ||
- | <img src="https://static.igem.org/mediawiki/2014/ | + | <img src="https://static.igem.org/mediawiki/2014/1/1c/Aldolase.PNG"> |
- | <h3> | + | <h3>K1392932 (Aldolase, 1014bp)</h3> |
- | <p> | + | <p> We got those photos after PCR of E. coli BL21 (DE3). We have tried colony and liquid culture of E coli. BL21 (DE3) </p> |
</td> | </td> | ||
Line 239: | Line 217: | ||
<td align="center" width="162px"><a href="http://www.etietieti.com/"> <img src="https://static.igem.org/mediawiki/2014/7/75/Eti.jpg" width="175px"></a></td> | <td align="center" width="162px"><a href="http://www.etietieti.com/"> <img src="https://static.igem.org/mediawiki/2014/7/75/Eti.jpg" width="175px"></a></td> | ||
+ | |||
+ | <td align="center"><a href="http://www.taa-ankara.org.tr/eng/index-eng.php"> <img src="https://static.igem.org/mediawiki/2014/0/00/Turkamerikan.jpg" width="175px"></a></td> | ||
+ | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | |||
<td align="center"><a href="http://www.biyans.com/"> <img src="https://static.igem.org/mediawiki/2014/a/a3/Biyans.PNG" width="175px"></a></td> | <td align="center"><a href="http://www.biyans.com/"> <img src="https://static.igem.org/mediawiki/2014/a/a3/Biyans.PNG" width="175px"></a></td> | ||
<td align="center"><a href="http://biyoakademi.org/"> <img src="https://static.igem.org/mediawiki/2014/b/b1/Biyoakademi.png" width="175px"></a></td> | <td align="center"><a href="http://biyoakademi.org/"> <img src="https://static.igem.org/mediawiki/2014/b/b1/Biyoakademi.png" width="175px"></a></td> | ||
- | |||
- | |||
<td align="center"><a href="http://www.sentegen.com/"> <img src="https://static.igem.org/mediawiki/2014/d/dd/Sentegenlogoder.PNG" width="175px"></a></td> | <td align="center"><a href="http://www.sentegen.com/"> <img src="https://static.igem.org/mediawiki/2014/d/dd/Sentegenlogoder.PNG" width="175px"></a></td> | ||
- | |||
- | |||
<td align="center"><a href="http://www.geneious.com/"> <img src="https://static.igem.org/mediawiki/2014/8/89/Geneious_Logo.gif" width="175px"></a></td> | <td align="center"><a href="http://www.geneious.com/"> <img src="https://static.igem.org/mediawiki/2014/8/89/Geneious_Logo.gif" width="175px"></a></td> | ||
Line 275: | Line 251: | ||
</tr> | </tr> | ||
</table> | </table> | ||
- | |||
</html> | </html> |
Latest revision as of 02:10, 18 October 2014
Team:METU Turkey/Templates/Navigationbar
HOME | TEAM | PROJECT | PARTS | MODELING | INTERLAB STUDY |
POLICY&PRACTICE | CHARACTERIZATION | SAFETY | ATTRIBUTIONS | GALLERY | PROTOCOLS |
![]() |
![]() |
|
|
|
|
![]() |
![]() |
![]() |
![]() |
![]() |
|
![]() |
|
![]() |
Our Supporter: | ||
|