Team:SYSU-China/file/Project/Notebook/Protocol/RNAT.html

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<h1>RNAT</h1>
<h1>RNAT</h1>
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<h2><b>Test Fluorescent</b></h2>
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<p>1. Begin culture the strains</p>
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<p>3mL three antibiotic LB culture per tube. </p>
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<p>Overnight，then add Arabinose to induce</p>
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<p>2. Prepare to test</p>
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    <p>Take 200μL from original culture to test OD</p>
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    <p>Discard the culture</p>
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    <p>Resuspend: OD=0.n add n*200 μL Yat-sen spring. (Because LB have very strong signal of green fluorescent)</p>
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<p>3. Test fluorescent using the Hybrid reader in the fifth floor</p>
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    <p>Take 200μL from each tube to test OD and fluorescent. (3 wells repeat) </p>
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    <p>Test 3 times in the machine.</p>
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<p><b>The parameters:</b></p>
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<p>OD=600</p>
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<p>Fluorescent: 485/528; 480/507; 510/550; 580/607</p>
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<p>Repeat the whole experiment 3 times. </p>
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Latest revision as of 02:04, 18 October 2014

RNAT

Test Fluorescent

1. Begin culture the strains

3mL three antibiotic LB culture per tube.

Overnight，then add Arabinose to induce

2. Prepare to test

Take 200μL from original culture to test OD

Discard the culture

Resuspend: OD=0.n add n*200 μL Yat-sen spring. (Because LB have very strong signal of green fluorescent)

3. Test fluorescent using the Hybrid reader in the fifth floor

Take 200μL from each tube to test OD and fluorescent. (3 wells repeat)

Test 3 times in the machine.

The parameters:

OD=600

Fluorescent: 485/528; 480/507; 510/550; 580/607

Repeat the whole experiment 3 times.



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