Team:ITESM-CEM/Parts
From 2014.igem.org
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<h2>Cholesterol Oxidase</h2> | <h2>Cholesterol Oxidase</h2> | ||
- | <p style="text-align: justify; text-justify: inter-word;"> This enzyme was first detected in <u>Chromobacterium sp.</u> We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1871 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin. | + | <p style="text-align: justify; text-justify: inter-word;"> This enzyme was first detected in <u>Chromobacterium sp.</u> We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1871 nucleotides and its codons were optimized in order to use it on <u>E. coli</u>, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin. |
</p> <br> | </p> <br> | ||
<h2>Oxoacyl Reductase</h2> | <h2>Oxoacyl Reductase</h2> | ||
- | <p style="text-align: justify; text-justify: inter-word;"> This enzyme was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1007 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin. | + | <p style="text-align: justify; text-justify: inter-word;"> This enzyme was detected in <u>Rhodococcus jostii </u>. We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1007 nucleotides and its codons were optimized in order to use it on <u>E. coli</u>, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin. |
</p> <br> | </p> <br> | ||
<h2>7-dehydratase</h2> | <h2>7-dehydratase</h2> | ||
- | <p style="text-align: justify; text-justify: inter-word;"> This enzyme (7-alpha dehydratase) was detected in Rhodococcus jostii . We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 602 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin. | + | <p style="text-align: justify; text-justify: inter-word;"> This enzyme (7-alpha dehydratase) was detected in <u>Rhodococcus jostii</u> . We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 602 nucleotides and its codons were optimized in order to use it on <u>E.coli</u>, it already included a stop codon for trancription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin. |
</p> <br> | </p> <br> | ||
<h2>Neomycin Resistance</h2> | <h2>Neomycin Resistance</h2> | ||
- | <p style="text-align: justify; text-justify: inter-word;"> This selective marker was | + | <p style="text-align: justify; text-justify: inter-word;"> This selective marker was obtained from an mammalian expression vector. NeoR's length is 855 nucleotides and it was isolated from pcDNA3.1(+)/myc-His A. |
</p><br> | </p><br> | ||
<h2>BGHPA</h2> | <h2>BGHPA</h2> | ||
- | <p style="text-align: justify; text-justify: inter-word;"> | + | <p style="text-align: justify; text-justify: inter-word;"> Bovine Growth Hormone Polyadenilation Signal for nuclease resistance. Translation terminator for eukaryotic cells. |
</p><br> | </p><br> | ||
<h2>PCMV</h2> | <h2>PCMV</h2> | ||
- | <p style="text-align: justify; text-justify: inter-word;"> | + | <p style="text-align: justify; text-justify: inter-word;"> Constitutive promoter from Cytomegalovirus, this promoter works on eukaryotic cells, driving protein expression. |
</p><br><br><br> | </p><br><br><br> | ||
Revision as of 02:03, 18 October 2014
ITESM-CEM | Enzy7-K me |
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