Team:NTU Taida/Circuit4
From 2014.igem.org
(Difference between revisions)
(Created page with "{{:Team:NTU_Taida/template/header}} {{:Team:NTU_Taida/template/css/bootstrap}} <!DOCTYPE html> <html lang="en"> <head> <meta charset="utf-8"> <meta http-equiv="X-UA-Co...") |
|||
(8 intermediate revisions not shown) | |||
Line 2: | Line 2: | ||
{{:Team:NTU_Taida/template/css/bootstrap}} | {{:Team:NTU_Taida/template/css/bootstrap}} | ||
- | |||
<html lang="en"> | <html lang="en"> | ||
<head> | <head> | ||
Line 11: | Line 10: | ||
<!-- Bootstrap --> | <!-- Bootstrap --> | ||
+ | |||
<link href='http://fonts.googleapis.com/css?family=Snippet|Cabin+Sketch:400,700|Flamenco|Shadows+Into+Light|Shadows+Into+Light+Two|Julius+Sans+One|Kranky|Megrim&subset=latin,latin-ext' rel='stylesheet' type='text/css'> | <link href='http://fonts.googleapis.com/css?family=Snippet|Cabin+Sketch:400,700|Flamenco|Shadows+Into+Light|Shadows+Into+Light+Two|Julius+Sans+One|Kranky|Megrim&subset=latin,latin-ext' rel='stylesheet' type='text/css'> | ||
<!-- HTML5 Shim and Respond.js IE8 support of HTML5 elements and media queries --> | <!-- HTML5 Shim and Respond.js IE8 support of HTML5 elements and media queries --> | ||
Line 19: | Line 19: | ||
<![endif]--> | <![endif]--> | ||
</head> | </head> | ||
- | <body> | + | <body id="top"> |
- | <nav class="navbar navbar-default navbar-fixed-top" role="navigation"> | + | <nav class="navbar navbar-default navbar-fixed-top" role="navigation" > |
<div class="container-fluid"> | <div class="container-fluid"> | ||
<!-- Brand and toggle get grouped for better mobile display --> | <!-- Brand and toggle get grouped for better mobile display --> | ||
Line 30: | Line 30: | ||
<span class="icon-bar"></span> | <span class="icon-bar"></span> | ||
</button> | </button> | ||
- | <a href=" | + | <a href="https://2014.igem.org/Team:NTU_Taida"><img id="logo" width="70px" src="https://static.igem.org/mediawiki/2014/1/19/NTU_Taida_logo.jpg"></a> |
</div> | </div> | ||
Line 36: | Line 36: | ||
<div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1"> | <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1"> | ||
<ul class="nav navbar-nav"> | <ul class="nav navbar-nav"> | ||
- | <li class="active"><a href="TEAM | + | <li class="active"><a href="TEAM">TEAM</a></li> |
Line 42: | Line 42: | ||
<a href="#" class="dropdown-toggle" data-toggle="dropdown">PROJECT<span class="caret"></span></a> | <a href="#" class="dropdown-toggle" data-toggle="dropdown">PROJECT<span class="caret"></span></a> | ||
<ul class="dropdown-menu" role="menu"> | <ul class="dropdown-menu" role="menu"> | ||
- | <li><a href="problem | + | <li><a href="problem">Problem (introduction)</a></li> |
- | <li><a href="PROJECT | + | <li><a href="PROJECT">Overview Mind Map</a></li> |
<li class="divider"></li> | <li class="divider"></li> | ||
- | <li><a href="Circuit1 | + | <li><a href="Circuit1">Fad System</a></li> |
<li class="divider"></li> | <li class="divider"></li> | ||
- | <li><a href="Circuit2 | + | <li><a href="Circuit2">Skin Whitening --- PKEK</a></li> |
<li class="divider"></li> | <li class="divider"></li> | ||
- | <li><a href="Circuit3 | + | <li><a href="Circuit3"> Corneum decomposition --- Keratinase</a></li> |
<li class="divider"></li> | <li class="divider"></li> | ||
- | <li><a href="Circuit4 | + | <li><a href="Circuit4">Fragrance</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
Line 61: | Line 61: | ||
<ul class="dropdown-menu" role="menu"> | <ul class="dropdown-menu" role="menu"> | ||
- | <li><a href="M1 | + | <li><a href="M1">Our Simulation Progress</a></li> |
<li class="divider"></li> | <li class="divider"></li> | ||
- | <li><a href="M2 | + | <li><a href="M2">Model 1</a></li> |
- | <li><a href="M3 | + | <li><a href="M3">Model 2</a></li> |
- | <li><a href="M4 | + | <li><a href="M4">Model 3</a></li> |
<li class="divider"></li> | <li class="divider"></li> | ||
- | <li><a href="M5 | + | <li><a href="M5">Curve fitting</a></li> |
<li class="divider"></li> | <li class="divider"></li> | ||
- | <li><a href="M6 | + | <li><a href="M6">Background knowledge</a></li> |
<li class="divider"></li> | <li class="divider"></li> | ||
- | <li><a href="M7 | + | <li><a href="M7">Reference</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
- | + | <li class="dropdown"> | |
- | <a href=" | + | <a href="SAFETY" >SAFETY</a> |
- | + | </li> | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
<li class="dropdown"> | <li class="dropdown"> | ||
- | <a href="PARTS | + | <a href="PARTS" >PARTS</a> |
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
- | <a href="LOGBOOK | + | <a href="LOGBOOK" >LOGBOOK</a> |
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
- | <a href="HP1 | + | <a href="HP1" >HUMAN PRACTICE</a> |
</li> | </li> | ||
+ | |||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown">SPONSORS<span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu" role="menu"> | ||
+ | <li><a href="Apex">ApexBio Biotech</a></li> | ||
+ | <li><a href="Lih">LihPao Life Science</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
<li class="active"> | <li class="active"> | ||
- | <a href="https://2014.igem.org/Main_Page"><img src=" | + | <a href="https://2014.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/6/6f/NTU_Taida_iGEM_logo.jpg" width=30px;> |
MAIN PAGE 2014 | MAIN PAGE 2014 | ||
</a> </li> | </a> </li> | ||
+ | |||
+ | <li class="active"> | ||
+ | <a href="https://www.facebook.com/NationalTaiwanUniversityiGEM?fref=ts"><img src="https://static.igem.org/mediawiki/2014/5/5a/NTU_Taida_Facebook.jpg" width=100px;> | ||
+ | </a></li> | ||
</ul> | </ul> | ||
Line 144: | Line 151: | ||
<div id="menuwrapper"> | <div id="menuwrapper"> | ||
<div id="menu"> | <div id="menu"> | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</div> | </div> | ||
Line 154: | Line 158: | ||
<div class="boxE"> | <div class="boxE"> | ||
- | <a href="#" > <img src=" | + | <a href="#" > <img src="https://static.igem.org/mediawiki/2014/e/ea/NTU_Taida_Fragrance_circuit.jpg" height="520px" align="center"> </a> |
</div> | </div> | ||
- | <div class="container"style="width:1200px;line-height:4em;padding:200px;padding-top:100px;padding-right:100px" float="left"> | + | <div class="container"style="width:1200px;line-height:4em;padding:200px;padding-top:100px;padding-right:100px" float="left" id="ins"> |
<div><center><h2 style="background-color:#eeeeee">Fragrance</h2></center></div> | <div><center><h2 style="background-color:#eeeeee">Fragrance</h2></center></div> | ||
Line 165: | Line 169: | ||
<li class="boxF"><h4 style="color:#00A0E9">Inspiration :</h4></p> | <li class="boxF"><h4 style="color:#00A0E9">Inspiration :</h4></p> | ||
<h5 style="line-height:1.4em;font-size:110%;"><b>GRASSY GREEN NOTES</b></p> | <h5 style="line-height:1.4em;font-size:110%;"><b>GRASSY GREEN NOTES</b></p> | ||
- | We want to turn fatty acids into volatile compounds that smells fresh and pleasant. First, we catalyze desaturation of Oleic acid(18:1 Δ9) into Linoleic acid(18:2 Δ9,12) by introducing a gene that encodesΔ12 Desaturase into <i>E.coli</i>.</p> | + | We want to turn fatty acids into volatile compounds that smells fresh and pleasant. First, we catalyze desaturation of Oleic acid(18:1 Δ9) into Linoleic acid(18:2 Δ9,12) by introducing a gene that encodesΔ12 Desaturase into <i>E.coli</i> (FAD6).</p> |
- | Then we catalyze oxidation and | + | Then we catalyze oxidation (LOX2) and hydrolysis (HPL1) of Linoleic acid(18:2 Δ9,12) to give a family of hexanal which smells fresh, generally known as “Grassy Green Notes”.</p></h5> |
- | <h5 style="line-height:1.4em;font-size:110%;"><b>ROSE FRAGRANCE</b></p> | + | <h5 style="line-height:1.4em;font-size:110%;"><b id="test">ROSE FRAGRANCE</b></p> |
Besides turning wasted fatty acids into fragrance, we also try to produce other fragrance from engineered E.coli. Our goal is to turn beta-carotene into rose fragrance by introducing a gene called CCD1 from Arabidopsis thaliana.</h5> | Besides turning wasted fatty acids into fragrance, we also try to produce other fragrance from engineered E.coli. Our goal is to turn beta-carotene into rose fragrance by introducing a gene called CCD1 from Arabidopsis thaliana.</h5> | ||
</li> | </li> | ||
Line 173: | Line 177: | ||
<li class="boxG"> | <li class="boxG"> | ||
<h4 style="color:#00A0E9">Testing Result :</h4></p> | <h4 style="color:#00A0E9">Testing Result :</h4></p> | ||
+ | <h4 style="color:gray"> | ||
+ | Function of Rose Fragrance Generator</h4> | ||
+ | <h5 style="line-height:1.4em;font-size:110%;"> | ||
+ | <strong style="font-size:110%">Method:</strong></p> | ||
+ | <p style="padding-bottom:30px">Submit beta-ionone standard and extract of our rose fragrance generator to Gas Chromatography (GC) respectively</p> | ||
+ | <strong style="font-size:110%;padding-bottom:30px">Result:</strong></p> | ||
+ | <figure style="padding-bottom:30px"> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/2/2a/NTU_Taida_F_test1.jpg" width="800px"> | ||
+ | <br/> | ||
+ | <figcaption>Beta-ionone standard</br> | ||
+ | We injected the beta-ionone standard, extracted by ethyl ehanol, into Gas chromotagraphy which existed an explicit curve at 27.5 minute. | ||
+ | </figcaption> | ||
+ | </figure> | ||
+ | <figure style="padding-bottom:30px"> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/7/76/NTU_Taida_F_tset_2.jpg" width="800px"> | ||
+ | <br/> | ||
+ | <figcaption>Extract of rose fragrance generator</br> | ||
+ | Then, we injected the sample which had been extracted by Ethyl ethanol also existed curves at around 27 minutes. However, the peak quality is not as good as standard. | ||
+ | </figcaption> | ||
+ | <br/> | ||
+ | <figcaption><b>Beta-ionone standard</b></figcaption> | ||
+ | <br/> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/3/33/NTU_Taida_Cc4.png" width="600px" style="padding-bottom:30px"> | ||
+ | <br/> | ||
+ | <figcaption><b>Extract of rose fragrance generator</b></figcaption> | ||
+ | <br/> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/1/13/NTU_Taida_Ccd5.png" width="600px"> | ||
+ | </figure> | ||
+ | <strong style="font-size:110%" id="back" id="back">Conclusion:</strong></p> | ||
+ | Success in generating rose fragrance beta-ionone from our genetic engineered <i>E.coli</i>.</p> | ||
</li> | </li> | ||
Line 178: | Line 212: | ||
<h4 style="color:#00A0E9">Background Knowledge :</h4></p> | <h4 style="color:#00A0E9">Background Knowledge :</h4></p> | ||
<h5 style="line-height:1.4em;font-size:110%;"> | <h5 style="line-height:1.4em;font-size:110%;"> | ||
- | Green notes are fresh and lively and they are used to make a fragrance feel crisp and sharp. Green notes very often include green leaves, tea leaves, the essence of freshly cut grass and even some marine plants. Green notes are most commonly used in sporty fragrances and summertime editions of popular perfumes. | + | <strong>Green notes</strong> are fresh and lively and they are used to make a fragrance feel crisp and sharp. Green notes very often include green leaves, tea leaves, the essence of freshly cut grass and even some marine plants. Green notes are most commonly used in sporty fragrances and summertime editions of popular perfumes. |
+ | </br> | ||
+ | |||
+ | <p><strong>Rose fragrance</strong></br> | ||
+ | <ol> | ||
+ | <li>E.coli strain MG1063: | ||
+ | <ul><li>F plasmid with a transposon, Tn1000. | ||
+ | <li>pSW140K with a beta-carotene synthesis operon. | ||
+ | <li>Kanamycin resistance.<br> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/b/b8/NTU_Taida_Cc1.png"><img src="https://static.igem.org/mediawiki/2014/2/2b/NTU_Taida_Cc2.png"> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <br> | ||
+ | <li>E.coli strain HB101: | ||
+ | <ul><li> Without F plasmid. | ||
+ | <li> pSW140K is stable in it. (Reference1) | ||
+ | </ul> | ||
+ | </li> | ||
+ | <br> | ||
+ | <li>Carotenoid cleavage dioxygenase 1 (CCD1): | ||
+ | <ul><li>From Arabidopsis thaliana. | ||
+ | <li>Turn beta-carotene into beta-ionone. | ||
+ | <li>Full length CDS: 1617 bp<br> | ||
+ | <image src="images/cc3.png"> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
+ | </ol> | ||
+ | |||
</h5> | </h5> | ||
</li> | </li> | ||
<li class="boxG"> | <li class="boxG"> | ||
- | <h4 style="color:#00A0E9">Reference :</h4></p> | + | <h4 style="color:#00A0E9" id="ref">Reference :</h4></p> |
<h5 style="line-height:1.4em;font-size:110%;"> | <h5 style="line-height:1.4em;font-size:110%;"> | ||
<a href="https://www.arabidopsis.org/servlets/TairObject?id=37898&type=locus">CCD1</a></p> | <a href="https://www.arabidopsis.org/servlets/TairObject?id=37898&type=locus">CCD1</a></p> | ||
+ | <ol> | ||
+ | <li>Mei-Hui Lin and Shih-Tung Liu (2008) J. Bacteriol 190(10):3681. | ||
+ | <li>To, Kin-Ying, et al. "Analysis of the gene cluster encoding carotenoid biosynthesis in Erwinia herbicola Eho13." Microbiology 140.2 (1994): 331-339. | ||
+ | <li>Cao, Hongbo, et al. "Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus." Journal of experimental botany (2012): ers115. | ||
+ | <li>http://cgsc.biology.yale.edu/Strain.php?ID=14667 | ||
+ | <li>http://www.leffingwell.com/rose.htm | ||
+ | </ol> | ||
<a href="https://www.arabidopsis.org/servlets/TairObject?id=128262&type=locus">FAD6</a></p> | <a href="https://www.arabidopsis.org/servlets/TairObject?id=128262&type=locus">FAD6</a></p> | ||
<a href="https://www.arabidopsis.org/servlets/TairObject?id=39538&type=locus">LOX2</a></p> | <a href="https://www.arabidopsis.org/servlets/TairObject?id=39538&type=locus">LOX2</a></p> | ||
<a href="https://www.arabidopsis.org/servlets/TairObject?id=128810&type=locus">HPL1</a></p> | <a href="https://www.arabidopsis.org/servlets/TairObject?id=128810&type=locus">HPL1</a></p> | ||
</h5> | </h5> | ||
- | + | <h5 style="line-height:1.4em;font-size:110%;"> | |
+ | Characterization of the yellow-pigment genes of | ||
+ | Erwinia herbicola Molecular Microbiology (1991) 5(1). 217'-224 | ||
+ | </p> | ||
+ | Stabilization of pSW100 from Pantoea stewartii by the F Conjugation System JOURNAL OF BACTERIOLOGY, May 2008, p. 3681–3689 Vol. 190, No. 10 | ||
+ | 0021-9193/08/$08.000 doi:10.1128/JB.00846-07</h5> | ||
+ | </li> | ||
+ | <li class="boxG"> | ||
+ | <h4 style="color:#00A0E9"><a href="#top">Top</a></p></h4> | ||
+ | </li> | ||
</li> | </li> | ||
Latest revision as of 01:50, 18 October 2014