Team:NTU Taida/Circuit3
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<strong>Degrading too much keratin</strong> might cause our skin become vulnerable to allergy and infection. Also, we don’t want keratinase being produced before the bacteria live on human skin. So our gene circuit must function like this:</p><p><img src="https://static.igem.org/mediawiki/2014/c/cc/NTU_Taida_Keratinase_model.jpg" style="width:360px;height:160px"></p><p><strong>The model</strong> can be simulated by these equations, which is based on Hill functions. The simulating result shows the expression of target protein is repressed effectively after a specific time interval.</p><p><img src="https://static.igem.org/mediawiki/2014/2/24/NTU_Taida_Keratinase_model_2.jpg" style="width:360px;height:160px"> | <strong>Degrading too much keratin</strong> might cause our skin become vulnerable to allergy and infection. Also, we don’t want keratinase being produced before the bacteria live on human skin. So our gene circuit must function like this:</p><p><img src="https://static.igem.org/mediawiki/2014/c/cc/NTU_Taida_Keratinase_model.jpg" style="width:360px;height:160px"></p><p><strong>The model</strong> can be simulated by these equations, which is based on Hill functions. The simulating result shows the expression of target protein is repressed effectively after a specific time interval.</p><p><img src="https://static.igem.org/mediawiki/2014/2/24/NTU_Taida_Keratinase_model_2.jpg" style="width:360px;height:160px"> | ||
</p><p><strong>It sould be</strong> activated by sensing fatty acid in environment and it has to be shut down after a specific time interval. The transcriptional cascade model composes a repressing protein, a promoter and a target protein, which could be green fluorescence protein, keratinase or any other protein. The repressing protein CI can shut down the promoter preceding the target protein, as long as it has accumulated to a threshold concentration.</p><p><img src="https://static.igem.org/mediawiki/2014/f/f0/NTU_Taida_Keratinase_model_3.jpg" style="width:360px;height:300px" ></p><div style="float:right"></p> | </p><p><strong>It sould be</strong> activated by sensing fatty acid in environment and it has to be shut down after a specific time interval. The transcriptional cascade model composes a repressing protein, a promoter and a target protein, which could be green fluorescence protein, keratinase or any other protein. The repressing protein CI can shut down the promoter preceding the target protein, as long as it has accumulated to a threshold concentration.</p><p><img src="https://static.igem.org/mediawiki/2014/f/f0/NTU_Taida_Keratinase_model_3.jpg" style="width:360px;height:300px" ></p><div style="float:right"></p> | ||
- | To secrete the enzyme outside the bacteria, a gene which functions as a signal sequence is inserted before the keratinase coding region. The signal peptide can guide the enzyme to the cell membrane. To further details, please check out the description | + | To secrete the enzyme outside the bacteria, a gene which functions as a signal sequence is inserted before the keratinase coding region. The signal peptide can guide the enzyme to the cell membrane. To further details, please check out the description in Skin Whitening --- PKEK page.</div></h5> |
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Latest revision as of 01:50, 18 October 2014