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Team:Evry/Notebook/Transformation/09-10-2014
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<u> <b> <big><FONT COLOR=#003333>Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation </font></big></b> </u> <br> | <u> <b> <big><FONT COLOR=#003333>Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation </font></big></b> </u> <br> | ||
A contamination has been shown in another transformation test. We can't use our previous results because there is a chance that it's not <i>Pseudovibrio</i> but the contamination which had been transformed. | A contamination has been shown in another transformation test. We can't use our previous results because there is a chance that it's not <i>Pseudovibrio</i> but the contamination which had been transformed. | ||
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Revision as of 01:49, 18 October 2014
Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation
A contamination has been shown in another transformation test. We can't use our previous results because there is a chance that it's not Pseudovibrio but the contamination which had been transformed.
Stock of pBBR1MCS and pRhokHI-2
The colony of E.Coli transformed in LB 1X + Cam (1:1000) culture of 3mL was sowed on new LB/LB+Cam(1:1000)/LB+Kan(1:1000) plates and incubated with shaking overnight at 37°C.
