Team:HIT-Harbin/Project
From 2014.igem.org
(6 intermediate revisions not shown) | |||
Line 31: | Line 31: | ||
</div> | </div> | ||
<!--kuang--> | <!--kuang--> | ||
- | <div class="nav1 home" ><a href="https:// | + | <div class="nav1 home" ><a href="https://igem.org/Main_Page" target="_blank"></a></div> |
<!--software--> | <!--software--> | ||
<div id="software" class="nav1" ><a href="https://2014.igem.org/Team:HIT-Harbin/Software">Software</a> | <div id="software" class="nav1" ><a href="https://2014.igem.org/Team:HIT-Harbin/Software">Software</a> | ||
Line 98: | Line 98: | ||
<h2>Project</h2> | <h2>Project</h2> | ||
</div> | </div> | ||
+ | <div style="margin: 0 auto; width: 600px"> | ||
+ | <img id="Family" width="600px" src="https://static.igem.org/mediawiki/2014/1/11/Abstract1.png"> | ||
+ | </div> | ||
<div class="multi-columns"> | <div class="multi-columns"> | ||
<div class="paragraphs"> | <div class="paragraphs"> | ||
<h5 class="hashed"><span><cufon class="cufon cufon-canvas" alt="Overview: " style="width: 59px; height: 18px;"><cufontext>Abstract </cufontext></cufon></span></h5> | <h5 class="hashed"><span><cufon class="cufon cufon-canvas" alt="Overview: " style="width: 59px; height: 18px;"><cufontext>Abstract </cufontext></cufon></span></h5> | ||
- | |||
<p>Dioxins, by-products of various industrial processes, are commonly regarded as highly toxic compounds that are environmental pollutants and persistent organic pollutants. Its deleterious hazards intensify with the odorless, colorless and fat-soluble properties, which enable dioxins to accumulate in vivo thus threaten almost the whole biosphere. Considering their ablilty of causing reproductive and developmental problems, damaging the immune system, interfering with hormones and also leading to cancer, hand in glove with the difficulty of being tested, dioxins are worthy of the name “poison of the century”.</p> | <p>Dioxins, by-products of various industrial processes, are commonly regarded as highly toxic compounds that are environmental pollutants and persistent organic pollutants. Its deleterious hazards intensify with the odorless, colorless and fat-soluble properties, which enable dioxins to accumulate in vivo thus threaten almost the whole biosphere. Considering their ablilty of causing reproductive and developmental problems, damaging the immune system, interfering with hormones and also leading to cancer, hand in glove with the difficulty of being tested, dioxins are worthy of the name “poison of the century”.</p> | ||
- | + | </div> | |
+ | </div> | ||
+ | <div style="margin: 0 auto; width: 600px"> | ||
<img id="Family" width="600px" src="https://static.igem.org/mediawiki/2014/7/77/Abstract3.png"> | <img id="Family" width="600px" src="https://static.igem.org/mediawiki/2014/7/77/Abstract3.png"> | ||
- | + | </div> | |
+ | <div class="multi-columns"> | ||
+ | <div class="paragraphs"> | ||
<p>So as to detect dioxins blisteringly, we have constructed device for rapid detection of dioxins inside yeast. We utlized associability between the gene for testing dioxins of MOUSE as receptor and dioxins as antibodies, together with the regulation conducted by lexA operator to downstream promoters so that mdr and lexA are combined, and, striding a step further, induce downstream gene with the presence of dioxins. Eventually, we can achieve our goal of detecting dioxins rapidly through the system of positive feedback of flourescin. Concerning the properties of yeast, like non-toxic and able to survive in hypertonic environment, our project can be put into wide application in the field like food detection, hence guarantee the food safety among the community of we human beings. | <p>So as to detect dioxins blisteringly, we have constructed device for rapid detection of dioxins inside yeast. We utlized associability between the gene for testing dioxins of MOUSE as receptor and dioxins as antibodies, together with the regulation conducted by lexA operator to downstream promoters so that mdr and lexA are combined, and, striding a step further, induce downstream gene with the presence of dioxins. Eventually, we can achieve our goal of detecting dioxins rapidly through the system of positive feedback of flourescin. Concerning the properties of yeast, like non-toxic and able to survive in hypertonic environment, our project can be put into wide application in the field like food detection, hence guarantee the food safety among the community of we human beings. | ||
</p> | </p> | ||
+ | </div> | ||
+ | <div id="title"> | ||
+ | <h2>Reference:</h2> | ||
+ | </div> | ||
+ | <div class="paragraphs"> | ||
+ | <p>1.Mimura J, Fujii-Kuriyama Y. Functional role of AhR in the expression of toxic effects by TCDD[J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 2003, 1619(3): 263-268. | ||
+ | </p> | ||
+ | <p>2.Denison M S, Heath-Pagliuso S. The Ah receptor: a regulator of the biochemical and toxicological actions of structurally diverse chemicals[J]. Bulletin of environmental contamination and toxicology, 1998, 61(5): 557-568. | ||
+ | </p> | ||
+ | <p>3.Toren A, Segal G, Ron E Z, et al. Structure–function studies of the recombinant protein bioemulsifier AlnA[J]. Environmental microbiology, 2002, 4(5): 257-261. | ||
+ | </p> | ||
+ | <p>4.Furukawa K, Fujihara H. Microbial degradation of polychlorinated biphenyls: biochemical and molecular features[J]. Journal of bioscience and bioengineering, 2008, 105(5): 433-449. | ||
+ | </p> | ||
+ | <p>5.Whitelaw M L, Göttlicher M, Gustafsson J A, et al. Definition of a novel ligand binding domain of a nuclear bHLH receptor: co-localization of ligand and hsp90 binding activities within the regulable inactivation domain of the dioxin receptor[J]. The EMBO journal, 1993, 12(11): 4169.</p> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 133: | Line 153: | ||
</a> | </a> | ||
</div> | </div> | ||
- | + | <div class="third last"> | |
- | <div class="third"> | + | |
<a href="http://life.hit.edu.cn//"> | <a href="http://life.hit.edu.cn//"> | ||
- | <img src=" | + | <img src="https://static.igem.org/mediawiki/2014/5/5e/Life.jpg"> |
</a> | </a> | ||
</div> | </div> | ||
+ | </div> | ||
+ | |||
</div> | </div> | ||
<div class="multi-columns"> | <div class="multi-columns"> | ||
Line 168: | Line 189: | ||
<img src="https://static.igem.org/mediawiki/2012/e/e5/J6.JPG"> | <img src="https://static.igem.org/mediawiki/2012/e/e5/J6.JPG"> | ||
</a> | </a> | ||
+ | |||
+ | |||
+ | </div> | ||
<div class="third last"> | <div class="third last"> | ||
<a href="http://hituc.hit.edu.cn:2011/out.do?viewType=outViewIndex"> | <a href="http://hituc.hit.edu.cn:2011/out.do?viewType=outViewIndex"> | ||
<img src="https://static.igem.org/mediawiki/2012/c/c5/J1.JPG"> | <img src="https://static.igem.org/mediawiki/2012/c/c5/J1.JPG"> | ||
</a> | </a> | ||
- | |||
- | |||
</div> | </div> | ||
<div id="bottomNav"><a href="#top">Back To Top</a></div> | <div id="bottomNav"><a href="#top">Back To Top</a></div> | ||
<script>toolbar('nav')</script> | <script>toolbar('nav')</script> |
Latest revision as of 01:47, 18 October 2014
Project
![](https://static.igem.org/mediawiki/2014/1/11/Abstract1.png)
Abstract
Dioxins, by-products of various industrial processes, are commonly regarded as highly toxic compounds that are environmental pollutants and persistent organic pollutants. Its deleterious hazards intensify with the odorless, colorless and fat-soluble properties, which enable dioxins to accumulate in vivo thus threaten almost the whole biosphere. Considering their ablilty of causing reproductive and developmental problems, damaging the immune system, interfering with hormones and also leading to cancer, hand in glove with the difficulty of being tested, dioxins are worthy of the name “poison of the century”.
![](https://static.igem.org/mediawiki/2014/7/77/Abstract3.png)
So as to detect dioxins blisteringly, we have constructed device for rapid detection of dioxins inside yeast. We utlized associability between the gene for testing dioxins of MOUSE as receptor and dioxins as antibodies, together with the regulation conducted by lexA operator to downstream promoters so that mdr and lexA are combined, and, striding a step further, induce downstream gene with the presence of dioxins. Eventually, we can achieve our goal of detecting dioxins rapidly through the system of positive feedback of flourescin. Concerning the properties of yeast, like non-toxic and able to survive in hypertonic environment, our project can be put into wide application in the field like food detection, hence guarantee the food safety among the community of we human beings.
Reference:
1.Mimura J, Fujii-Kuriyama Y. Functional role of AhR in the expression of toxic effects by TCDD[J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 2003, 1619(3): 263-268.
2.Denison M S, Heath-Pagliuso S. The Ah receptor: a regulator of the biochemical and toxicological actions of structurally diverse chemicals[J]. Bulletin of environmental contamination and toxicology, 1998, 61(5): 557-568.
3.Toren A, Segal G, Ron E Z, et al. Structure–function studies of the recombinant protein bioemulsifier AlnA[J]. Environmental microbiology, 2002, 4(5): 257-261.
4.Furukawa K, Fujihara H. Microbial degradation of polychlorinated biphenyls: biochemical and molecular features[J]. Journal of bioscience and bioengineering, 2008, 105(5): 433-449.
5.Whitelaw M L, Göttlicher M, Gustafsson J A, et al. Definition of a novel ligand binding domain of a nuclear bHLH receptor: co-localization of ligand and hsp90 binding activities within the regulable inactivation domain of the dioxin receptor[J]. The EMBO journal, 1993, 12(11): 4169.
Click here and get more details about our project.