Team:Utah State

From 2014.igem.org

(Difference between revisions)
 
(16 intermediate revisions not shown)
Line 3: Line 3:
<html>
<html>
-
 
+
<body></body>
-
<!--main content -->
+
-
 
+
<div class="page">
<div class="page">
-
  <ul class="dropdown">
+
<h2> Project Abstract </h2>
-
      <li class="parent">
+
<p>
-
      <a class="menu-link" href="">
+
Laundry detergents are one of the most commonly used household cleaning products in today’s society because they effectively clean and remove stains from clothing. Even though they are relatively harmless to humans, laundry detergents contain caustic chemicals and reagents that have a detrimental impact on the environment. Our aim is to use enzymatic means of cleaning to reduce the amount of detergent necessary for normal washes as well as increase the efficiency of stain removal. We have engineered <i>E. coli</i> to produce enzymes that can be used to remove laundry stains such as grass, starch, and oils. By functionalizing these enzymes on a bioplastic material that can be used as an additive to traditional laundry cycles, we hope to improve the sanitation of clothing and other washable materials. The demonstration of enzyme functionality after biological immobilization will create a platform for future teams to display enzymes relevant to other applications.
-
        <ul class="dropdown">
+
</p>
-
            <li>
+
-
              <a class="menu-link">Introduction</a>
+
-
            </li>
+
-
            <li>
+
-
              <a class="menu-link">Designs</a>
+
-
            </li>
+
-
            <li>
+
-
              <a class="menu-link">Applications</a>
+
-
            </li>         
+
-
        </ul>
+
-
      </a>
+
-
      </li>
+
-
  </ul>
+
-
</div>
+
-
<table align="center">
+
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
<h2></h2>
-
<!-- end welcome box -->
+
-
<tr>  
+
-
<!--navigation menu -->
+
<p>
-
<td align="center" colspan="3">
+
<h3>Can you imagine...</h3>
 +
</p>
 +
<p>a life without clean clothes? Oh, the smell alone! Not to mention the unsightly colors and spots that would accumulate from your native food and flora. Detergents help run the world of personal hygiene and first impressions. Nobody wants a bad memory left from our appearance. Although detergents have ruled our laundry closets and will remain a household staple, innovations ought to transpire to create more ecologically friendly formulas. Who better to learn from than the creator of those stains, nature herself? Enzymes are nature’s catalyst to either create or destroy bonds, to break down those stains.
 +
</p>
-
<table  width="100%">
+
<p>
-
<tr heigth="15px"></tr>
+
There is a need for this change because of the impact of detergent on water quality. Logan city is an example. Surrounded by the beautiful mountains of Cache valley, Utah State University is one of the gems of Logan city. In the middle of the valley, hidden by wetlands and marsh, are over 1 billion gallons worth of lagoons holding Logan city’s waste water. These lagoons treat the water through a series of natural processes. The out stream only needs to be lightly chlorinated before returning to the environment. This solution has worked for decades until recently when nutrient levels of the out stream remained too high, nutrients including phosphate [1,2]. Phosphates can be found in most laundry detergents, shampoos, and fertilizer.
-
<tr heigth="75px">  
+
</p>
 +
<p>
 +
The hazards of phosphate in detergents has been known to producers since the 1980’s [3]. Only recently have leading companies made enough progress to announce intentions to completely remove phosphates from their formulas [4]. Although progress has been made, new innovations must continue. With our project solution there is also potential to help third world countries without access to detergent or populations where phosphate regulation is disregarded.
 +
</p>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
<h2> References </h2>
-
<a href="https://2014.igem.org/Team:Utah_State"style="color:#000000">Home </a> </td>
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<p>
-
<a href="https://2014.igem.org/Team:Utah_State/Team"style="color:#000000"> Team </a> </td>
+
[1] http://www.loganutah.org/Environmental/Waste%20Water%20Treatment/  
 +
</p>
-
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<p>
-
<a href="https://igem.org/Team.cgi?year=2014&team_name=Utah_State"style="color:#000000"> Official Team Profile </a></td>
+
[2] “City of Logan: Wastewater Treatment Master Plan Update 2013”
 +
</p>
-
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
<p>
-
<a href="https://2014.igem.org/Team:Utah_State/Project"style="color:#000000"> Project</a></td>
+
[3] https://www.deq.idaho.gov/media/892716-dishwaster-detergent-phosphorus-pilot-study-1101.pdf
 +
</p>
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<p>
-
<a href="https://2014.igem.org/Team:Utah_State/Parts"style="color:#000000"> Parts</a></td>
+
[4] http://www.greenbiz.com/blog/2014/01/28/pg-aims-remove-phosphates-leading-detergent-brands
 +
</p>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
<br>
-
<a href="https://2014.igem.org/Team:Utah_State/Modeling"style="color:#000000"> Modeling</a></td>
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
</div>
-
<a href="https://2014.igem.org/Team:Utah_State/Notebook"style="color:#000000"> Notebook</a></td>
+
</html>
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:Utah_State/Safety"style=" color:#000000"> Safety </a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:Utah_State/Attributions"style="color:#000000"> Attributions </a></td>
+
-
 
+
-
 
+
-
<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
<!--end navigation menu -->
+
-
</tr>
+
-
 
+
-
 
+
-
</tr>
+
-
+
-
 
+
-
 
+
-
 
+
-
 
+
-
</td>
+
-
 
+
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
-
 
+
-
 
+
-
<!--requirements section -->
+
-
<tr><td colspan="3"> <h3> Requirements </h3></td></tr>
+
-
<tr>
+
-
<td width="45%"  valign="top">
+
-
 
+
-
<p> Please be sure to keep these links, your audience will want to find your: </p>
+
-
 
+
-
<!-- Links to other team pages -->
+
-
<ul>
+
-
<li><a href="https://2014.igem.org/Team:Utah_State">Home</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Utah_State/Team">Team</a> </li>
+
-
<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Utah_State">Official Team Profile</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Utah_State/Project">Project</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Utah_State/Parts">Parts</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Utah_State/Modeling">Modeling</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Utah_State/Notebook">Notebook</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Utah_State/Safety">Safety</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Utah_State/Attributions">Attributions</a> </li>
+
-
 
+
-
</ul>
+
-
 
+
-
</td>
+
-
 
+
-
<td > </td>
+
-
<td width="45%">
+
-
 
+
-
<p>There are a few wiki requirements teams must follow:</p>
+
-
<ul>
+
-
<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
+
-
<li>All pages must be created under the team’s name space.</li>
+
-
<li>As part of your documentation, keep the links from the menu to the left. </li>
+
-
<li>Do not use flash in wiki code. </li>
+
-
<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
+
-
</ul>
+
-
<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
+
-
 
+
-
</td>
+
-
</tr>
+
-
 
+
-
 
+
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
-
 
+
-
 
+
-
<!--project description section -->
+
-
<tr><td colspan="3"> <h3> Project Description </h3></td></tr>
+
-
<tr>
+
-
<td width="45%"  valign="top">
+
-
<p><b>The 2014 Utah State University iGEM team aims to engineer <i>E. coli</i> to eliminate tough laundry stains using enzymes that can break down various stains (e.g. oils, proteins, starches, pigments). By biologically immobilizing these enzymes onto bacterially-produced bioplastic, we plan to create a reusable stain-fighting device to be used as a pre-treatment or as an additive to traditional laundry. </b></p>
+
-
 
+
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>
+
-
 
+
-
 
+
-
<!--tips  -->
+
-
<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
+
-
 
+
-
<tr>
+
-
<td width="45%" valign="top">
+
-
<p>We are currently working on providing teams with some easy to use design templates.
+
-
<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
+
-
 
+
-
<ul>
+
-
<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
+
-
<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
+
-
<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
+
-
<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
+
-
<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
+
-
</ul>
+
-
 
+
-
<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
+
-
</td>
+
-
 
+
-
<td> </td>
+
-
<td width="45%">
+
-
 
+
-
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
-
 
+
-
<ul>
+
-
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
-
<li>Be clear about what you are doing and what you plan to do.</li>
+
-
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
-
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
-
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
-
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
+
-
<li>Have lots of fun! </li>
+
-
</ul>
+
-
</br>
+
-
</td>
+
-
</tr>
+
-
</table>
+

Latest revision as of 01:45, 18 October 2014

Project Abstract

Laundry detergents are one of the most commonly used household cleaning products in today’s society because they effectively clean and remove stains from clothing. Even though they are relatively harmless to humans, laundry detergents contain caustic chemicals and reagents that have a detrimental impact on the environment. Our aim is to use enzymatic means of cleaning to reduce the amount of detergent necessary for normal washes as well as increase the efficiency of stain removal. We have engineered E. coli to produce enzymes that can be used to remove laundry stains such as grass, starch, and oils. By functionalizing these enzymes on a bioplastic material that can be used as an additive to traditional laundry cycles, we hope to improve the sanitation of clothing and other washable materials. The demonstration of enzyme functionality after biological immobilization will create a platform for future teams to display enzymes relevant to other applications.

Can you imagine...

a life without clean clothes? Oh, the smell alone! Not to mention the unsightly colors and spots that would accumulate from your native food and flora. Detergents help run the world of personal hygiene and first impressions. Nobody wants a bad memory left from our appearance. Although detergents have ruled our laundry closets and will remain a household staple, innovations ought to transpire to create more ecologically friendly formulas. Who better to learn from than the creator of those stains, nature herself? Enzymes are nature’s catalyst to either create or destroy bonds, to break down those stains.

There is a need for this change because of the impact of detergent on water quality. Logan city is an example. Surrounded by the beautiful mountains of Cache valley, Utah State University is one of the gems of Logan city. In the middle of the valley, hidden by wetlands and marsh, are over 1 billion gallons worth of lagoons holding Logan city’s waste water. These lagoons treat the water through a series of natural processes. The out stream only needs to be lightly chlorinated before returning to the environment. This solution has worked for decades until recently when nutrient levels of the out stream remained too high, nutrients including phosphate [1,2]. Phosphates can be found in most laundry detergents, shampoos, and fertilizer.

The hazards of phosphate in detergents has been known to producers since the 1980’s [3]. Only recently have leading companies made enough progress to announce intentions to completely remove phosphates from their formulas [4]. Although progress has been made, new innovations must continue. With our project solution there is also potential to help third world countries without access to detergent or populations where phosphate regulation is disregarded.

References

[1] http://www.loganutah.org/Environmental/Waste%20Water%20Treatment/

[2] “City of Logan: Wastewater Treatment Master Plan Update 2013”

[3] https://www.deq.idaho.gov/media/892716-dishwaster-detergent-phosphorus-pilot-study-1101.pdf

[4] http://www.greenbiz.com/blog/2014/01/28/pg-aims-remove-phosphates-leading-detergent-brands