Team:Groningen/Template/MODULE/Notebook/bandage/week6

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Fig. 3: our L.lactis bacteria, left is GFP and right is a normal look
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The <i>L. lactis</i> NZ9000 strain with pNZ8048g under the microscope, on the left the GFP channel is shown, and on the right the  phase contrast picture is displayed
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11 August - 15 August
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August 11 - August 15
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Again we poured several gels with the GFP strain. These were induced (this time we incubated this for over 2 hours, the best result is reached after 2 hours.) and checked under the microscope. We finally seen some induced cells under the microscope.
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A polyacrylamide gel with the <i>L. lactis</i> NZ9000 strain with pNZ8048g, we incubated the gels in GM17 media with ZnSO4 for two hours at 30 °C, and observed the gels under the microscope
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Some GFP expressing <i>L. lactis</i> was seen under the microscope
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We will still try to repeat this process several times. We'd like to be sure our cells can survive inside the the acrylamid. and also if it survives the solidifying process.
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Repeating the experiment looking for the best conditions
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Latest revision as of 01:39, 18 October 2014

Figure 2
 
Figure 2: The L. lactis NZ9000 strain with pNZ8048g under the microscope, on the left the GFP channel is shown, and on the right the phase contrast picture is displayed
 
 
August 11 - August 15
 
A polyacrylamide gel with the L. lactis NZ9000 strain with pNZ8048g, we incubated the gels in GM17 media with ZnSO4 for two hours at 30 °C, and observed the gels under the microscope
 
Some GFP expressing L. lactis was seen under the microscope
 
Repeating the experiment looking for the best conditions
 
 
 
 

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