Team:Groningen/Template/MODULE/Notebook/bandage/week4

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28 July - 1 August
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July 28 - August 1
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Thanks to dongdong Mu we've received a l.lactis strain with GFP (pNZ8048G). We've made our own stock from this and created some overnight cultures for more experiments.
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Preparing a GFP stock of <i>L. lactis</i> NZ9000 with pNZ8048g, and growing an overnight culture of this strain
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Incubated the GFP strain with ZnSO4 with several concentrations to check when GFP is shown the best.
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Inducing the <i>L. lactis</i> NZ9000 with pNZ8048g with different concentrations of ZnSO4, the cells were observed under the microscope and some GFP expression was observed
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This was checked under the microscope which gave some nice pictures
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The <i>L. lactis</i> NZ9000 strain with pNZ8048g under the microscope, on the left the GFP channel is shown, and on the right the ordinary phase contrast picture is displayed
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test 3
 
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Latest revision as of 01:33, 18 October 2014

July 28 - August 1
 
Preparing a GFP stock of L. lactis NZ9000 with pNZ8048g, and growing an overnight culture of this strain
 
Inducing the L. lactis NZ9000 with pNZ8048g with different concentrations of ZnSO4, the cells were observed under the microscope and some GFP expression was observed
 
Figure 1
 
Figure 1: The L. lactis NZ9000 strain with pNZ8048g under the microscope, on the left the GFP channel is shown, and on the right the ordinary phase contrast picture is displayed
 
 
 
 
 

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