Team:Groningen/Template/MODULE/Notebook/bandage/week4

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week 4
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July 28 - August 1
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In silico preparation of primers for the Gibson assembly between signal sequence USP45 and modified version of Aiia.
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Preparing a GFP stock of <i>L. lactis</i> NZ9000 with pNZ8048g, and growing an overnight culture of this strain
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In silico production of synthetic gene ssUSP45DspB.
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Inducing the <i>L. lactis</i> NZ9000 with pNZ8048g with different concentrations of ZnSO4, the cells were observed under the microscope and some GFP expression was observed
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The <i>L. lactis</i> NZ9000 strain with pNZ8048g under the microscope, on the left the GFP channel is shown, and on the right the ordinary phase contrast picture is displayed
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General preparation of lab necessities.
 
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Latest revision as of 01:33, 18 October 2014

July 28 - August 1
 
Preparing a GFP stock of L. lactis NZ9000 with pNZ8048g, and growing an overnight culture of this strain
 
Inducing the L. lactis NZ9000 with pNZ8048g with different concentrations of ZnSO4, the cells were observed under the microscope and some GFP expression was observed
 
Figure 1
 
Figure 1: The L. lactis NZ9000 strain with pNZ8048g under the microscope, on the left the GFP channel is shown, and on the right the ordinary phase contrast picture is displayed
 
 
 
 
 

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