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Team:Utah State/Results/Cullolase
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| + | <img src="https://static.igem.org/mediawiki/2014/4/43/USU2014_Cellulase-web.png" width="450" height="300" alt="USU 2014iGem2014;" /> | ||
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<h2> Mechanism </h2> | <h2> Mechanism </h2> | ||
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Cellulase is the primary enzyme responsible for the cleavage of cellulose. Cellulose is a linear polysaccharide made up of chained glucose units and very abundant in the natural world being the prinicpal component of plant cell walls. It differs from starch by its 1,4 b acetal linkage which results in its incompatibility with amylase. Cellulase acts by breaking the glucose chains freeing the glucose monosaccharides. The most common producers of natural cellulase are fungi and certain bacteria and protists. According to current research, <i>E. coli</i> does not naturally produce cellulase but other intestine dwelling microbes do, chiefly those found in herbivores. The use of cellulase in the detergent industry is primarily in brightening of colors and whitening in cotton fabrics. This is due to the degradation of microfibrils that have separated from the main weave over time and use. Cellulase can also be utilized in the production of paper, pulp-less fruit juices, biofuels, and liquor. Essentially, cellulase can be used in any process that requires the degradation of plant fibers or cell walls. | Cellulase is the primary enzyme responsible for the cleavage of cellulose. Cellulose is a linear polysaccharide made up of chained glucose units and very abundant in the natural world being the prinicpal component of plant cell walls. It differs from starch by its 1,4 b acetal linkage which results in its incompatibility with amylase. Cellulase acts by breaking the glucose chains freeing the glucose monosaccharides. The most common producers of natural cellulase are fungi and certain bacteria and protists. According to current research, <i>E. coli</i> does not naturally produce cellulase but other intestine dwelling microbes do, chiefly those found in herbivores. The use of cellulase in the detergent industry is primarily in brightening of colors and whitening in cotton fabrics. This is due to the degradation of microfibrils that have separated from the main weave over time and use. Cellulase can also be utilized in the production of paper, pulp-less fruit juices, biofuels, and liquor. Essentially, cellulase can be used in any process that requires the degradation of plant fibers or cell walls. | ||
</p> | </p> | ||
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| - | <img src="https://static.igem.org/mediawiki/2014/a/ | + | <h2> Results </h2> |
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| + | <h4> Cellulase Generator Construction </h4> | ||
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| + | <p> | ||
| + | Protein generator part for chlorophyllase. Part BBa_K118023 was obtained from the parts registry and is the cenA coding sequence encoding Cellulomonas fimi endoglucanase A. In order to be compatible for fusion protein applications, primers were designed to make BBa_K118023 RCF 23 compatible. The product of this PCR reaction was cloned into pSB1C3 and is designated as BBa_K1418010. The double stop codons were then removed via PCR, cloned into pSB1C3 and this construct is designated as BBa_K1418011. Part BBa_K1418011 was then cloned behind a lac inducible promoter and a ribosome binding site (K208010 contains both R0010 and B0034). To aid in protein purification, a 10x histidine tag with two transcriptional terminators (K844000) was cloned in frame on the 3' end to generate the final composite construct, BBa_K1418012. | ||
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| + | <br> | ||
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| + | <div align="center"> | ||
| + | <div style="width: 500px;"> | ||
| + | <img align="center" src="https://static.igem.org/mediawiki/2014/8/87/2014USU_K1418012PlasmidPic.png" width="656" height="51" alt="USU 2014iGem2014;" /> | ||
| + | </div> | ||
| + | </div> | ||
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| + | <br> | ||
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| + | <h4> Cellulase Purification </h4> | ||
| + | |||
| + | <p> | ||
| + | To test for protein production from the new BBa_K1418012 construct, protein purification using a nickel column was performed. Using methods provided in our "protocols" section, cells containing BBa_K1418012 were grown overnight, pelleted and lysed. After centrifugation, the supernatant was applied to the nickel column. Various samples throughout the purification process were analyzed using SDS-PAGE. The SDS-PAGE below shows results from analysis of cells containing BBa_K1418012. | ||
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| + | <br> | ||
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| + | <div align="center"> | ||
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| + | <img align="center" src="https://static.igem.org/mediawiki/2014/a/a0/2014USU_AnnotatedCenAProteinGel.png" width="325" height="324" alt="USU 2014iGem2014;" /> | ||
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| + | </div> | ||
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| + | <br> | ||
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| + | <p> | ||
| + | It can be seen from the SDS-PAGE that a protein product between 37 kDa and 50 kDa has been purified been purified using the nickel column. Since the expected size of our cellulase construct is 46 kDa, we are confident that we have purified the cellulase enzyme. | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | To test for enzymatic activity, extracts used in lanes 2-8 of the SDS-PAGE shown above were applied to a carboxymethyl cellulose plate and then stained with Congo Red according to methods detailed in our "protocols" page. | ||
| + | </p> | ||
| + | |||
| + | <div align="center"> | ||
| + | <div style="width: 500px;"> | ||
| + | <img align="center" src="https://static.igem.org/mediawiki/2014/8/80/2014USU_CongoRedPlateImage.PNG" width="312" height="275" alt="USU 2014iGem2014;" /> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <p> | ||
| + | From this result, it can be observed that extracts from the elution fractions (6-8) had more cellulase activity than fractions 2-5 (flow through and washes). The purified cellulase fractions had similar activity as the positive control, which contained 1mg/ml of commercial cellulase. | ||
| + | </p> | ||
| - | <h2> | + | <h2> References </h2> |
| - | + | ||
</div> | </div> | ||
</html> | </html> | ||
Latest revision as of 01:27, 18 October 2014
Mechanism
Cellulase is the primary enzyme responsible for the cleavage of cellulose. Cellulose is a linear polysaccharide made up of chained glucose units and very abundant in the natural world being the prinicpal component of plant cell walls. It differs from starch by its 1,4 b acetal linkage which results in its incompatibility with amylase. Cellulase acts by breaking the glucose chains freeing the glucose monosaccharides. The most common producers of natural cellulase are fungi and certain bacteria and protists. According to current research, E. coli does not naturally produce cellulase but other intestine dwelling microbes do, chiefly those found in herbivores. The use of cellulase in the detergent industry is primarily in brightening of colors and whitening in cotton fabrics. This is due to the degradation of microfibrils that have separated from the main weave over time and use. Cellulase can also be utilized in the production of paper, pulp-less fruit juices, biofuels, and liquor. Essentially, cellulase can be used in any process that requires the degradation of plant fibers or cell walls.
Results
Cellulase Generator Construction
Protein generator part for chlorophyllase. Part BBa_K118023 was obtained from the parts registry and is the cenA coding sequence encoding Cellulomonas fimi endoglucanase A. In order to be compatible for fusion protein applications, primers were designed to make BBa_K118023 RCF 23 compatible. The product of this PCR reaction was cloned into pSB1C3 and is designated as BBa_K1418010. The double stop codons were then removed via PCR, cloned into pSB1C3 and this construct is designated as BBa_K1418011. Part BBa_K1418011 was then cloned behind a lac inducible promoter and a ribosome binding site (K208010 contains both R0010 and B0034). To aid in protein purification, a 10x histidine tag with two transcriptional terminators (K844000) was cloned in frame on the 3' end to generate the final composite construct, BBa_K1418012.
Cellulase Purification
To test for protein production from the new BBa_K1418012 construct, protein purification using a nickel column was performed. Using methods provided in our "protocols" section, cells containing BBa_K1418012 were grown overnight, pelleted and lysed. After centrifugation, the supernatant was applied to the nickel column. Various samples throughout the purification process were analyzed using SDS-PAGE. The SDS-PAGE below shows results from analysis of cells containing BBa_K1418012.
It can be seen from the SDS-PAGE that a protein product between 37 kDa and 50 kDa has been purified been purified using the nickel column. Since the expected size of our cellulase construct is 46 kDa, we are confident that we have purified the cellulase enzyme.
To test for enzymatic activity, extracts used in lanes 2-8 of the SDS-PAGE shown above were applied to a carboxymethyl cellulose plate and then stained with Congo Red according to methods detailed in our "protocols" page.
From this result, it can be observed that extracts from the elution fractions (6-8) had more cellulase activity than fractions 2-5 (flow through and washes). The purified cellulase fractions had similar activity as the positive control, which contained 1mg/ml of commercial cellulase.
