Team:Groningen/Template/MODULE/Notebook/toolbox/appendix/table1

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Primers for making BioBricks out of the nisin operon
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  <tr>  
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<th>type</th><th>size (mm)</th>
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    <th>Primer</th>  
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    <th>Sequence</th>  
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  </tr>
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<td>colony</td><td>4,0</td>
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  <tr>
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</tr>
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    <td>NisA forward</td>
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    <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA</td>
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<td>colony with halo</td><td>6,0</td>
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  </tr>
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  <tr>
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    <td>NisA reverse</td>
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<td>halo</td><td>2,0</td>
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    <td>GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA</td>
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  </tr>
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    <td>NisB forward</td>
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    <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAAAAGTTCATTTAA</td>
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  </tr>
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  <tr>
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    <td>NisB reverse</td>
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    <td>GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCATGTATTCTT</td>
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  </tr>
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  <tr>
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    <td>NisT forward</td>
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    <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA</td>
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  </tr>
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  <tr>
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    <td>NisT reverse</td>
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    <td>GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT</td>
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  </tr>
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  <tr>
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    <td>NisC forward</td>
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    <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATAAAAAAAATATAAA</td>
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  </tr>
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  <tr>
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    <td>NisC reverse</td>
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    <td>GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCCTCTTCCCTC</td>
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  </tr> 
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  <tr>
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    <td>NisI forward</td>
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    <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGAAGATATTTAATACT</td>
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  </tr>
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  <tr>
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    <td>NisI reverse</td>
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    <td>GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTAATTTCCTACCTTCG</td>
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  </tr> 
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  <tr>
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    <td>NisP forward</td>
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    <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAAAAAATACTAGGTTT</td>
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  </tr> 
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  <tr>
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    <td>NisP reverse</td>
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    <td>GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC</td>
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  </tr> 
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  <tr>
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    <td>NisRK forward</td>
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    <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGTATAAAATTTTAATAGT</td>
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  </tr> 
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  <tr>
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    <td>NisRK reverse</td>
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    <td>GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA</td>
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  </tr> 
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  <tr>
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    <td>NisFEG forward</td>
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    <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA</td>
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  </tr> 
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  <tr>
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    <td>NisFEG reverse</td>
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    <td>GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT</td>
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  </tr> 
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  <tr>
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    <td>PNisI+RBS forward</td>
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    <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCACTGGATAATGACTATT</td>
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  </tr>
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  <tr>
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    <td>PNisI+RBS reverse</td>
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    <td>TACTAGTAGCGGCCGCTGCAGGAAGAAACTTCCTCTTCCCTCCTTTCAA</td>
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  </tr> 
</table>
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Latest revision as of 01:21, 18 October 2014

Primers for making BioBricks out of the nisin operon
 
Primer Sequence
NisA forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA
NisA reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA
NisB forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAAAAGTTCATTTAA
NisB reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCATGTATTCTT
NisT forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA
NisT reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT
NisC forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATAAAAAAAATATAAA
NisC reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCCTCTTCCCTC
NisI forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGAAGATATTTAATACT
NisI reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTAATTTCCTACCTTCG
NisP forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAAAAAATACTAGGTTT
NisP reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC
NisRK forward GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGTATAAAATTTTAATAGT
NisRK reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA
NisFEG forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA
NisFEG reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT
PNisI+RBS forward GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCACTGGATAATGACTATT
PNisI+RBS reverse TACTAGTAGCGGCCGCTGCAGGAAGAAACTTCCTCTTCCCTCCTTTCAA
 
 
 
 

Retrieved from "http://2014.igem.org/Team:Groningen/Template/MODULE/Notebook/toolbox/appendix/table1"