Team:Oxford/notebook

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_NOTOC__
 
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<h1>Lab Book</h1>
 
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<p> Information will be posted here soon! - Watch this space</p>
 
-
 
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<h1>Modelling</h1>
 
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<h2>Model of part B possible repression and activation network scenarios</h2>
 
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<div class="news_block">
 
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<p>Finished the first draft of the model, will leave it until we have real data to feed back into the system. The model is very robust and allows any user to input a large variety of parameters and scenarios that could be realistically expected in the laboratory results. The output of the model is the colour that you can expect over time (the outputs of the real system will be from a combination of mCherry and GFP).</p>
 
-
<p>The model reveals surprising results, including how even a small basal rate of gene expression (due to leakage of the promoters) can really change the results.</p>
 
-
<p>Information on how to use Matlab to model repressor and activator networks very easily, accurately and quickly will be uploaded to this wiki soon! If you want more details please don't hesitate to contact us.</p>
 
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</div>
 
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</html>
 
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[[File:Model 1.png|900px|thumb|left|An example of the model's output]]
 
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<html>
 
-
 
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<h2>[DCM] calibration for solution-vapour equilibration</h2>
 
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<div class="news_block">
 
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<p>Testing the tolerance of E-coli and Pseudomonas strains to various concentrations of DCM (0mM, 5mM, 10mM, 20mM), the 15 mL falcon tube-contained system is modeled as a real binary solution/ideal gas, based on literature solubility data<sup>1</sup>, confirming negligible [DCM] deviation and the validity of our approximation:</p></div>
 
-
 
-
<img src="http://latex.codecogs.com/gif.latex?p_{i}=c*k^{\Theta&space;}e^{\frac{\mathrm{d}&space;lnk}{\mathrm{d}&space;1/T}(\frac{1}{T}-\frac{1}{298.15K})}" title="p_{i}=c*k^{\Theta }e^{\frac{\mathrm{d}&space;lnk}{\mathrm{d}&space;1/T}(\frac{1}{T}-\frac{1}{298.15K})}" /><br>
 
-
 
-
 
-
<img src="http://latex.codecogs.com/gif.latex?k^\Theta=3.9*10^{-6}&space;\:&space;mol&space;\:&space;kg^{-1}&space;Pa^{-1},&space;\frac{\mathrm{d}&space;lnk}{\mathrm{d}&space;1/T}=&space;3900&space;\:K" title="k^\Theta=3.9*10^{-6} \: mol \: kg^{-1} Pa^{-1}, \frac{\mathrm{d} lnk}{\mathrm{d} 1/T}= 3900 \:K" />
 
-
 
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</html>
 
-
[[File:Model 1.png]]
 
-
<html>
 
-
 
-
<p class="p1"><br></p>
 
-
 
-
<table cellspacing="0" cellpadding="0" class="t1">
 
-
  <tbody>
 
-
    <tr>
 
-
      <td valign="top" class="td1">
 
-
        <p class="p1"><br></p>
 
-
      </td>
 
-
      <td valign="top" class="td2">
 
-
        <p class="p2"><span class="s1">V(DCM)</span><span class="s2"><sub> </sub></span><span class="s1">added (± 5E-4) /ml</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td3">
 
-
        <p class="p2"><span class="s1">n(DCM)</span><span class="s2"><sub>(l)</sub></span><span class="s1"> unadjusted (± 8E-6) /mol</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td4">
 
-
        <p class="p2"><span class="s1">[DCM] unadjusted (± 2E-3) /M</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td5">
 
-
        <p class="p2"><span class="s1">p(DCM)<span class="Apple-converted-space"> </span></span></p>
 
-
        <p class="p2"><span class="s1">(± 4E-2) /kPa</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td6">
 
-
        <p class="p2"><span class="s1">n(DCM)</span><span class="s2"><sub>(g)</sub></span></p>
 
-
        <p class="p2"><span class="s1">(± 4E-7) /mol</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td7">
 
-
        <p class="p2"><span class="s1">n(DCM)</span><span class="s2"><sub>(l)</sub></span><span class="s1"> (± 9E-6) adjusted /mol</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td2">
 
-
        <p class="p2"><span class="s1">[DCM] adjusted<span class="Apple-converted-space"> </span></span></p>
 
-
        <p class="p2"><span class="s1">(± 2E-3) /M</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td8">
 
-
        <p class="p2"><span class="s1">Δ[DCM] /M</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td9">
 
-
        <p class="p2"><span class="s1">[DCM] % deviation</span></p>
 
-
      </td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td valign="top" class="td10">
 
-
        <p class="p2"><span class="s1"><b>(30°C, 5mM)</b></span></p>
 
-
      </td>
 
-
      <td valign="top" class="td11">
 
-
        <p class="p3"><span class="s1">1.6E-03</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td12">
 
-
        <p class="p3"><span class="s1">2.5E-05</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td13">
 
-
        <p class="p3"><span class="s1">5.0E-03</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td14">
 
-
        <p class="p3"><span class="s1">6.2E-01</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td15">
 
-
        <p class="p3"><span class="s1">1.6E-08</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td16">
 
-
        <p class="p3"><span class="s1">2.5E-05</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td17">
 
-
        <p class="p3"><span class="s1"><b>5.0E-03</b></span></p>
 
-
      </td>
 
-
      <td valign="top" class="td18">
 
-
        <p class="p3"><span class="s1">3.1E-06</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td19">
 
-
        <p class="p3"><span class="s1">6.3E-02</span></p>
 
-
      </td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td valign="top" class="td20">
 
-
        <p class="p2"><span class="s1"><b>(30°C, 10mM)</b></span></p>
 
-
      </td>
 
-
      <td valign="top" class="td21">
 
-
        <p class="p3"><span class="s1">3.2E-03</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td22">
 
-
        <p class="p3"><span class="s1">5.0E-05</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td23">
 
-
        <p class="p3"><span class="s1">1.0E-02</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td24">
 
-
        <p class="p3"><span class="s1">3.1E-01</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td25">
 
-
        <p class="p3"><span class="s1">7.9E-09</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td26">
 
-
        <p class="p3"><span class="s1">5.0E-05</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td27">
 
-
        <p class="p3"><span class="s1"><b>1.0E-02</b></span></p>
 
-
      </td>
 
-
      <td valign="top" class="td28">
 
-
        <p class="p3"><span class="s1">1.6E-06</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td29">
 
-
        <p class="p3"><span class="s1">1.6E-02</span></p>
 
-
      </td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td valign="top" class="td20">
 
-
        <p class="p2"><span class="s1"><b>(30°C, 20mM)</b></span></p>
 
-
      </td>
 
-
      <td valign="top" class="td21">
 
-
        <p class="p3"><span class="s1">6.4E-03</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td22">
 
-
        <p class="p3"><span class="s1">1.0E-04</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td23">
 
-
        <p class="p3"><span class="s1">2.0E-02</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td24">
 
-
        <p class="p3"><span class="s1">1.6E-01</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td25">
 
-
        <p class="p3"><span class="s1">3.9E-09</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td26">
 
-
        <p class="p3"><span class="s1">1.0E-04</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td27">
 
-
        <p class="p3"><span class="s1"><b>2.0E-02</b></span></p>
 
-
      </td>
 
-
      <td valign="top" class="td28">
 
-
        <p class="p3"><span class="s1">7.9E-07</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td29">
 
-
        <p class="p3"><span class="s1">3.9E-03</span></p>
 
-
      </td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td valign="top" class="td10">
 
-
        <p class="p1"><br></p>
 
-
      </td>
 
-
      <td valign="top" class="td30">
 
-
        <p class="p1"><br></p>
 
-
      </td>
 
-
      <td valign="top" class="td12">
 
-
        <p class="p1"><br></p>
 
-
      </td>
 
-
      <td valign="top" class="td13">
 
-
        <p class="p1"><br></p>
 
-
      </td>
 
-
      <td valign="top" class="td14">
 
-
        <p class="p1"><br></p>
 
-
      </td>
 
-
      <td valign="top" class="td15">
 
-
        <p class="p1"><br></p>
 
-
      </td>
 
-
      <td valign="top" class="td16">
 
-
        <p class="p1"><br></p>
 
-
      </td>
 
-
      <td valign="top" class="td30">
 
-
        <p class="p1"><br></p>
 
-
      </td>
 
-
      <td valign="top" class="td18">
 
-
        <p class="p1"><br></p>
 
-
      </td>
 
-
      <td valign="top" class="td19">
 
-
        <p class="p1"><br></p>
 
-
      </td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td valign="top" class="td20">
 
-
        <p class="p2"><span class="s1"><b>(37°C, 5mM)</b></span></p>
 
-
      </td>
 
-
      <td valign="top" class="td21">
 
-
        <p class="p3"><span class="s1">1.6E-03</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td22">
 
-
        <p class="p3"><span class="s1">2.5E-05</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td23">
 
-
        <p class="p3"><span class="s1">5.0E-03</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td24">
 
-
        <p class="p3"><span class="s1">6.2E-01</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td25">
 
-
        <p class="p3"><span class="s1">1.5E-08</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td26">
 
-
        <p class="p3"><span class="s1">2.5E-05</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td27">
 
-
        <p class="p3"><span class="s1"><b>5.0E-03</b></span></p>
 
-
      </td>
 
-
      <td valign="top" class="td28">
 
-
        <p class="p3"><span class="s1">3.1E-06</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td29">
 
-
        <p class="p3"><span class="s1">6.2E-02</span></p>
 
-
      </td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td valign="top" class="td20">
 
-
        <p class="p2"><span class="s1"><b>(37°C, 10mM)</b></span></p>
 
-
      </td>
 
-
      <td valign="top" class="td21">
 
-
        <p class="p3"><span class="s1">3.2E-03</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td22">
 
-
        <p class="p3"><span class="s1">5.0E-05</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td23">
 
-
        <p class="p3"><span class="s1">1.0E-02</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td24">
 
-
        <p class="p3"><span class="s1">3.1E-01</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td25">
 
-
        <p class="p3"><span class="s1">7.7E-09</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td26">
 
-
        <p class="p3"><span class="s1">5.0E-05</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td27">
 
-
        <p class="p3"><span class="s1"><b>1.0E-02</b></span></p>
 
-
      </td>
 
-
      <td valign="top" class="td28">
 
-
        <p class="p3"><span class="s1">1.5E-06</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td29">
 
-
        <p class="p3"><span class="s1">1.5E-02</span></p>
 
-
      </td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td valign="top" class="td10">
 
-
        <p class="p2"><span class="s1"><b>(37°C, 20mM)</b></span></p>
 
-
      </td>
 
-
      <td valign="top" class="td11">
 
-
        <p class="p3"><span class="s1">6.4E-03</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td12">
 
-
        <p class="p3"><span class="s1">1.0E-04</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td13">
 
-
        <p class="p3"><span class="s1">2.0E-02</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td14">
 
-
        <p class="p3"><span class="s1">1.6E-01</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td15">
 
-
        <p class="p3"><span class="s1">3.8E-09</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td16">
 
-
        <p class="p3"><span class="s1">1.0E-04</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td17">
 
-
        <p class="p3"><span class="s1"><b>2.0E-02</b></span></p>
 
-
      </td>
 
-
      <td valign="top" class="td18">
 
-
        <p class="p3"><span class="s1">7.7E-07</span></p>
 
-
      </td>
 
-
      <td valign="top" class="td19">
 
-
        <p class="p3"><span class="s1">3.9E-03</span></p>
 
-
      </td>
 
-
    </tr>
 
-
  </tbody>
 
-
</table>
 
-
 
-
<p class="p1"><br></p>
 
-
 
-
</body>
 
-
</div>
 
-
 
-
</html>
 
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-
</div>
 

Latest revision as of 01:03, 18 October 2014