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| - | <body>
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| - |
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| - | <h1>Lab Book</h1>
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| - |
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| - | <p> Information will be posted here soon! - Watch this space</p>
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| - |
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| - | <h1>Modelling</h1>
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| - | <h2>Model of part B possible repression and activation network scenarios</h2>
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| - | <p>Finished the first draft of the model, will leave it until we have real data to feed back into the system. The model is very robust and allows any user to input a large variety of parameters and scenarios that could be realistically expected in the laboratory results. The output of the model is the colour that you can expect over time (the outputs of the real system will be from a combination of mCherry and GFP).</p>
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| - |
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| - | <div class="news_block">
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| - | <h1>[DCM] calibration for solution-vapour equilibration</h1>
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| - | <p>Testing the tolerance of E-coli and Pseudomonas strains to various concentrations of DCM (0mM, 5mM, 10mM, 20mM), the 15 mL falcon tube-contained system is modeled as a real binary solution/ideal gas, based on literature solubility data<sup>1</sup>, confirming negligible [DCM] deviation:</p>
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| - |
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| - | <img src="http://latex.codecogs.com/gif.latex?p_{i}=c*k^{\Theta&space;}e^{\frac{\mathrm{d}&space;lnk}{\mathrm{d}&space;1/T}(\frac{1}{T}-\frac{1}{298.15K})}" title="p_{i}=c*k^{\Theta }e^{\frac{\mathrm{d}&space;lnk}{\mathrm{d}&space;1/T}(\frac{1}{T}-\frac{1}{298.15K})}" /><br>
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| - |
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| - |
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| - | <img src="http://latex.codecogs.com/gif.latex?k^\Theta=3.9*10^{-6}&space;\:&space;mol&space;\:&space;kg^{-1}&space;Pa^{-1},&space;\frac{\mathrm{d}&space;lnk}{\mathrm{d}&space;1/T}=&space;3900&space;\:K" title="k^\Theta=3.9*10^{-6} \: mol \: kg^{-1} Pa^{-1}, \frac{\mathrm{d} lnk}{\mathrm{d} 1/T}= 3900 \:K" />
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| - |
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| - | </div>
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| - |
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| - | <p class="p1"><br></p>
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| - |
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| - | <table cellspacing="0" cellpadding="0" class="t1">
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| - | <tbody>
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| - | <tr>
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| - | <td valign="top" class="td1">
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| - | <p class="p1"><br></p>
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| - | </td>
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| - | <td valign="top" class="td2">
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| - | <p class="p2"><span class="s1">V(DCM)</span><span class="s2"><sub> </sub></span><span class="s1">added (± 5E-4) /ml</span></p>
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| - | </td>
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| - | <td valign="top" class="td3">
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| - | <p class="p2"><span class="s1">n(DCM)</span><span class="s2"><sub>(l)</sub></span><span class="s1"> unadjusted (± 8E-6) /mol</span></p>
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| - | </td>
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| - | <td valign="top" class="td4">
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| - | <p class="p2"><span class="s1">[DCM] unadjusted (± 2E-3) /M</span></p>
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| - | </td>
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| - | <td valign="top" class="td5">
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| - | <p class="p2"><span class="s1">p(DCM)<span class="Apple-converted-space"> </span></span></p>
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| - | <p class="p2"><span class="s1">(± 4E-2) /kPa</span></p>
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| - | </td>
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| - | <td valign="top" class="td6">
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| - | <p class="p2"><span class="s1">n(DCM)</span><span class="s2"><sub>(g)</sub></span></p>
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| - | <p class="p2"><span class="s1">(± 4E-7) /mol</span></p>
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| - | </td>
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| - | <td valign="top" class="td7">
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| - | <p class="p2"><span class="s1">n(DCM)</span><span class="s2"><sub>(l)</sub></span><span class="s1"> (± 9E-6) adjusted /mol</span></p>
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| - | </td>
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| - | <td valign="top" class="td2">
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| - | <p class="p2"><span class="s1">[DCM] adjusted<span class="Apple-converted-space"> </span></span></p>
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| - | <p class="p2"><span class="s1">(± 2E-3) /M</span></p>
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| - | </td>
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| - | <td valign="top" class="td8">
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| - | <p class="p2"><span class="s1">Δ[DCM] /M</span></p>
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| - | </td>
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| - | <td valign="top" class="td9">
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| - | <p class="p2"><span class="s1">[DCM] % deviation</span></p>
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| - | </td>
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| - | </tr>
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| - | <tr>
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| - | <td valign="top" class="td10">
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| - | <p class="p2"><span class="s1"><b>(30°C, 5mM)</b></span></p>
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| - | </td>
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| - | <td valign="top" class="td11">
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| - | <p class="p3"><span class="s1">1.6E-03</span></p>
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| - | </td>
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| - | <td valign="top" class="td12">
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| - | <p class="p3"><span class="s1">2.5E-05</span></p>
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| - | </td>
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| - | <td valign="top" class="td13">
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| - | <p class="p3"><span class="s1">5.0E-03</span></p>
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| - | </td>
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| - | <td valign="top" class="td14">
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| - | <p class="p3"><span class="s1">6.2E-01</span></p>
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| - | </td>
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| - | <td valign="top" class="td15">
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| - | <p class="p3"><span class="s1">1.6E-08</span></p>
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| - | </td>
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| - | <td valign="top" class="td16">
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| - | <p class="p3"><span class="s1">2.5E-05</span></p>
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| - | </td>
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| - | <td valign="top" class="td17">
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| - | <p class="p3"><span class="s1"><b>5.0E-03</b></span></p>
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| - | </td>
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| - | <td valign="top" class="td18">
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| - | <p class="p3"><span class="s1">3.1E-06</span></p>
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| - | </td>
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| - | <td valign="top" class="td19">
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| - | <p class="p3"><span class="s1">6.3E-02</span></p>
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| - | </td>
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| - | </tr>
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| - | <tr>
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| - | <td valign="top" class="td20">
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| - | <p class="p2"><span class="s1"><b>(30°C, 10mM)</b></span></p>
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| - | </td>
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| - | <td valign="top" class="td21">
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| - | <p class="p3"><span class="s1">3.2E-03</span></p>
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| - | </td>
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| - | <td valign="top" class="td22">
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| - | <p class="p3"><span class="s1">5.0E-05</span></p>
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| - | </td>
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| - | <td valign="top" class="td23">
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| - | <p class="p3"><span class="s1">1.0E-02</span></p>
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| - | </td>
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| - | <td valign="top" class="td24">
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| - | <p class="p3"><span class="s1">3.1E-01</span></p>
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| - | </td>
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| - | <td valign="top" class="td25">
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| - | <p class="p3"><span class="s1">7.9E-09</span></p>
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| - | </td>
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| - | <td valign="top" class="td26">
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| - | <p class="p3"><span class="s1">5.0E-05</span></p>
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| - | </td>
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| - | <td valign="top" class="td27">
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| - | <p class="p3"><span class="s1"><b>1.0E-02</b></span></p>
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| - | </td>
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| - | <td valign="top" class="td28">
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| - | <p class="p3"><span class="s1">1.6E-06</span></p>
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| - | </td>
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| - | <td valign="top" class="td29">
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| - | <p class="p3"><span class="s1">1.6E-02</span></p>
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| - | </td>
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| - | </tr>
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| - | <tr>
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| - | <td valign="top" class="td20">
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| - | <p class="p2"><span class="s1"><b>(30°C, 20mM)</b></span></p>
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| - | </td>
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| - | <td valign="top" class="td21">
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| - | <p class="p3"><span class="s1">6.4E-03</span></p>
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| - | </td>
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| - | <td valign="top" class="td22">
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| - | <p class="p3"><span class="s1">1.0E-04</span></p>
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| - | </td>
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| - | <td valign="top" class="td23">
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| - | <p class="p3"><span class="s1">2.0E-02</span></p>
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| - | </td>
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| - | <td valign="top" class="td24">
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| - | <p class="p3"><span class="s1">1.6E-01</span></p>
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| - | </td>
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| - | <td valign="top" class="td25">
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| - | <p class="p3"><span class="s1">3.9E-09</span></p>
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| - | </td>
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| - | <td valign="top" class="td26">
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| - | <p class="p3"><span class="s1">1.0E-04</span></p>
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| - | </td>
| |
| - | <td valign="top" class="td27">
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| - | <p class="p3"><span class="s1"><b>2.0E-02</b></span></p>
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| - | </td>
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| - | <td valign="top" class="td28">
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| - | <p class="p3"><span class="s1">7.9E-07</span></p>
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| - | </td>
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| - | <td valign="top" class="td29">
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| - | <p class="p3"><span class="s1">3.9E-03</span></p>
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| - | </td>
| |
| - | </tr>
| |
| - | <tr>
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| - | <td valign="top" class="td10">
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| - | <p class="p1"><br></p>
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| - | </td>
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| - | <td valign="top" class="td30">
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| - | <p class="p1"><br></p>
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| - | </td>
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| - | <td valign="top" class="td12">
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| - | <p class="p1"><br></p>
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| - | </td>
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| - | <td valign="top" class="td13">
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| - | <p class="p1"><br></p>
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| - | </td>
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| - | <td valign="top" class="td14">
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| - | <p class="p1"><br></p>
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| - | </td>
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| - | <td valign="top" class="td15">
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| - | <p class="p1"><br></p>
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| - | </td>
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| - | <td valign="top" class="td16">
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| - | <p class="p1"><br></p>
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| - | </td>
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| - | <td valign="top" class="td30">
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| - | <p class="p1"><br></p>
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| - | </td>
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| - | <td valign="top" class="td18">
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| - | <p class="p1"><br></p>
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| - | </td>
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| - | <td valign="top" class="td19">
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| - | <p class="p1"><br></p>
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| - | </td>
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| - | </tr>
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| - | <tr>
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| - | <td valign="top" class="td20">
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| - | <p class="p2"><span class="s1"><b>(37°C, 5mM)</b></span></p>
| |
| - | </td>
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| - | <td valign="top" class="td21">
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| - | <p class="p3"><span class="s1">1.6E-03</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td22">
| |
| - | <p class="p3"><span class="s1">2.5E-05</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td23">
| |
| - | <p class="p3"><span class="s1">5.0E-03</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td24">
| |
| - | <p class="p3"><span class="s1">6.2E-01</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td25">
| |
| - | <p class="p3"><span class="s1">1.5E-08</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td26">
| |
| - | <p class="p3"><span class="s1">2.5E-05</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td27">
| |
| - | <p class="p3"><span class="s1"><b>5.0E-03</b></span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td28">
| |
| - | <p class="p3"><span class="s1">3.1E-06</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td29">
| |
| - | <p class="p3"><span class="s1">6.2E-02</span></p>
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| - | </td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td valign="top" class="td20">
| |
| - | <p class="p2"><span class="s1"><b>(37°C, 10mM)</b></span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td21">
| |
| - | <p class="p3"><span class="s1">3.2E-03</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td22">
| |
| - | <p class="p3"><span class="s1">5.0E-05</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td23">
| |
| - | <p class="p3"><span class="s1">1.0E-02</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td24">
| |
| - | <p class="p3"><span class="s1">3.1E-01</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td25">
| |
| - | <p class="p3"><span class="s1">7.7E-09</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td26">
| |
| - | <p class="p3"><span class="s1">5.0E-05</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td27">
| |
| - | <p class="p3"><span class="s1"><b>1.0E-02</b></span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td28">
| |
| - | <p class="p3"><span class="s1">1.5E-06</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td29">
| |
| - | <p class="p3"><span class="s1">1.5E-02</span></p>
| |
| - | </td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td valign="top" class="td10">
| |
| - | <p class="p2"><span class="s1"><b>(37°C, 20mM)</b></span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td11">
| |
| - | <p class="p3"><span class="s1">6.4E-03</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td12">
| |
| - | <p class="p3"><span class="s1">1.0E-04</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td13">
| |
| - | <p class="p3"><span class="s1">2.0E-02</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td14">
| |
| - | <p class="p3"><span class="s1">1.6E-01</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td15">
| |
| - | <p class="p3"><span class="s1">3.8E-09</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td16">
| |
| - | <p class="p3"><span class="s1">1.0E-04</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td17">
| |
| - | <p class="p3"><span class="s1"><b>2.0E-02</b></span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td18">
| |
| - | <p class="p3"><span class="s1">7.7E-07</span></p>
| |
| - | </td>
| |
| - | <td valign="top" class="td19">
| |
| - | <p class="p3"><span class="s1">3.9E-03</span></p>
| |
| - | </td>
| |
| - | </tr>
| |
| - | </tbody>
| |
| - | </table>
| |
| - |
| |
| - | <p class="p1"><br></p>
| |
| - |
| |
| - | <p>More detailed system modelling is therefore unnecessary.</p>
| |
| - |
| |
| - | </body>
| |
| - | </div>
| |
| - |
| |
| - | </html>
| |
| - | {{:Team:Oxford/templates/footer}}
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| - | </div>
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