|
|
| (5 intermediate revisions not shown) |
| Line 13: |
Line 13: |
| | <ul class="menu"> | | <ul class="menu"> |
| | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li> | | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li> |
| - | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan</a> </li> | + | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan Timer</a> </li> |
| | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Biohack_project">Biohacker Kit </a></li> | | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Biohack_project">Biohacker Kit </a></li> |
| | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Hardware">OpenSource Hardware </a> </li> | | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Hardware">OpenSource Hardware </a> </li> |
| | + | <!--<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Modeling">Modeling </a> </li>--> |
| | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Parts">BioBrick Parts </a></li> | | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Parts">BioBrick Parts </a></li> |
| - | <!--<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Modeling">Modeling </a> </li>-->
| |
| | </ul> | | </ul> |
| | </li> | | </li> |
| - | <li class="menu-safety"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Safety">Safety</a> </li> | + | <li class="menu-safety"> Social |
| - | <li class="menu -outreach"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Outreach">Outreach </a></li> | + | <ul class="menu"> |
| | + | <li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Safety">Safety</a> </li> |
| | + | <li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Ethics">Ethics</a> </li> |
| | + | <li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Outreach">Outreach </a> </li> |
| | + | </ul> |
| | + | </li> |
| | <li class="menu-notebook"> Notebook | | <li class="menu-notebook"> Notebook |
| | <ul class="menu"> | | <ul class="menu"> |
| Line 54: |
Line 59: |
| | <h2>Planned Parts</h2> | | <h2>Planned Parts</h2> |
| | | | |
| - | The scope of our project may be vast, but we only submitted parts we were able to successfully characterize. A lot of other components were considered from each subproject that did not make it due to time constraints and Murphy's Law.<br><br> | + | The scope of our project may be vast, but we only submitted parts we were able to successfully characterize. A lot of other components were considered from each sub-project that did not make it due to time constraints (and a bit of Murphy's Law). |
| - | <!-- De Novo Synthesis - TdT paper with truncated parts improved efficiency
| + | <br><br>For De Novo Project, primers for truncated TdT enzymes with the BioBrick prefixes were synthesized and used. Referring to the literature, first 81bp, 429bp, and 450bp of TdT were deleted to see the change in efficiency of the TdT's enzymatic function. <br><br>The Programmable Lifespan Timer had a collection of genes knocked out, including EST2, MAK31 and RAD52<br><br> |
| - | Programmable Lifespan Timer - Knock out cassettes for EST2, MAK31 and RAD52; Galactosidase inducable CRE Recombinase
| + | This summer, four more inputs and outputs were built by The Cooper Union Summer STEM iGEM high school students. The promoters they used were induced by the presence of arsenic, arabinose, lactone and glucose, and the outputs were RFP, YFP, blue chromoprotein, and turquoise chromoprotein. Some of these systems were designed for practical, real world applications - to detect poisons or help control diabetes by measuring glucose levels. |
| - | Biohacker Kit - Assembly of multiple inputs and outputs, as well as different promoters consideration -->
| + | <br><br> |
| | + | |
| | | | |
| | | | |
| Line 84: |
Line 90: |
| | <span>Projects</span> | | <span>Projects</span> |
| | </a> | | </a> |
| - | <a href="https://2014.igem.org/Team:Cooper_Union/Safety" class="red" > | + | <a href="https://2014.igem.org/Team:Cooper_Union/Social" class="red" > |
| - | <img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Safety" /> | + | <img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Social" /> |
| - | <span>Safety</span> | + | <span>Social</span> |
| - | </a>
| + | |
| - | <a href="https://2014.igem.org/Team:Cooper_Union/Outreach" class="yellow">
| + | |
| - | <img src="https://static.igem.org/mediawiki/2014/7/74/CU_2014_logoO.png" height="100" style="vertical-align: top;" title="Outreach" />
| + | |
| - | <span>Outreach</span>
| + | |
| | </a> | | </a> |
| | <a href="https://2014.igem.org/Team:Cooper_Union/Notebook" class="green" > | | <a href="https://2014.igem.org/Team:Cooper_Union/Notebook" class="green" > |
Tested, Submitted BioBrick Parts
Planned Parts
The scope of our project may be vast, but we only submitted parts we were able to successfully characterize. A lot of other components were considered from each sub-project that did not make it due to time constraints (and a bit of Murphy's Law).
For De Novo Project, primers for truncated TdT enzymes with the BioBrick prefixes were synthesized and used. Referring to the literature, first 81bp, 429bp, and 450bp of TdT were deleted to see the change in efficiency of the TdT's enzymatic function.
The Programmable Lifespan Timer had a collection of genes knocked out, including EST2, MAK31 and RAD52
This summer, four more inputs and outputs were built by The Cooper Union Summer STEM iGEM high school students. The promoters they used were induced by the presence of arsenic, arabinose, lactone and glucose, and the outputs were RFP, YFP, blue chromoprotein, and turquoise chromoprotein. Some of these systems were designed for practical, real world applications - to detect poisons or help control diabetes by measuring glucose levels.
"
