Team:Groningen/Template/MODULE/Notebook/secretion/week11

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<b>goal:</b> place the anti <i>Pseudomonas Aeruginosa</i> system in <i>Lactococcus lactis NZ9800</i>.
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Placing the <i>Pseudomonas Aeruginosa</i> system in <i>Lactococcus lactis</i> NZ9800
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We digested the anti <i>Pseudomonas aeruginosa</i> system and pIL253 vector with EcoRI & PstI.
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The <i>Pseudomonas aeruginosa</i> system (detection system fused with the secretion system) and pIL253 vector was digested with EcoRI & PstI
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Then 20 ul of ligation mix was dialysed for 1 h with dH2O. the dialysed sample was transformed into <i>L. lactis NZ9800</i> using electroporation.
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The <i>Pseudomonas aeruginosa</i> system was ligated with the pIL253 vector, and transformed to <i>L. lactis</i> NZ9800
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Revision as of 00:58, 18 October 2014

October 13 - October 16
 
Placing the Pseudomonas Aeruginosa system in Lactococcus lactis NZ9800
 
The Pseudomonas aeruginosa system (detection system fused with the secretion system) and pIL253 vector was digested with EcoRI & PstI
 
The Pseudomonas aeruginosa system was ligated with the pIL253 vector, and transformed to L. lactis NZ9800