|
|
(36 intermediate revisions not shown) |
Line 1: |
Line 1: |
- | == OpenGelBox == | + | <html> |
| + | <link rel="stylesheet" href="https://2014.igem.org/Team:Cooper_Union/CSS?action=raw&ctype=text/css" type="text/css" /> |
| + | <title>Cooper Union 2014 iGEM</title> |
| + | <body> |
| | | |
| + | <div class="parent-container"> |
| | | |
- | (picture)
| + | <div class="menu-parent"> |
| | | |
| + | <ul class="menu"> |
| + | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union"> Home </a> </li> |
| + | <li class="menu-project"> Project |
| + | <ul class="menu"> |
| + | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li> |
| + | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan Timer</a> </li> |
| + | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Biohack_project">Biohacker Kit </a></li> |
| + | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Hardware">OpenSource Hardware </a> </li> |
| + | <!--<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Modeling">Modeling </a> </li>--> |
| + | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Parts">BioBrick Parts </a></li> |
| + | </ul> |
| + | </li> |
| + | <li class="menu-safety"> Social |
| + | <ul class="menu"> |
| + | <li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Safety">Safety</a> </li> |
| + | <li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Ethics">Ethics</a> </li> |
| + | <li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Outreach">Outreach </a></li> |
| + | </ul> |
| + | </li> |
| + | <li class="menu-notebook"> Notebook |
| + | <ul class="menu"> |
| + | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Protocols">Commonly Used Protocols </li> |
| + | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Notebook"> Labnotes </a> </li> |
| + | </ul> |
| + | </li> |
| + | <li class="menu-team"> Team |
| + | <ul class="menu"> |
| + | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/biofanfic">Members </a> </li> |
| + | <li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Attributions"> Attributions </a> </li> |
| + | <li class="menu"> <a class="menu" href="https://igem.org/Team.cgi?id=1354"> Official Team Profile </a> </li> |
| + | </ul> |
| + | </li> |
| + | </ul> |
| + | <a class="menu" href="http://www.igem.org" border="0"> |
| + | <img src="https://static.igem.org/mediawiki/igem.org/9/94/Igemlogo_officiallogo.png" class="menu-top-logo" /> |
| + | </a> |
| | | |
- | == Introduction == | + | </div> |
| + | <br> |
| + | <hr> |
| + | <br> |
| + | <div class="text"> |
| + | <!-----Add content below----> |
| | | |
- | The gel box is an open source lab equipment design. This design was created by Karlin Yeh and Wilfrido Castillo and a version of it is in the Cooper Union. This design is made from acrylic sheet.
| |
| | | |
| + | <div class="center"> |
| + | <br><h1>Hardware</h1><br> |
| + | <div class="hardware"> |
| + | <img src="https://static.igem.org/mediawiki/2014/0/0b/CU_2014_OpenGel.png" title="The Cooper Union OpenGelBox" height="100px" width="100px" /><br> |
| + | <span class="hardware"><a href="https://2014.igem.org/Team:Cooper_Union/OpenGel">OpenGelBox</a></span> |
| + | </div> |
| + | <div class="hardware"> |
| + | <img src="https://static.igem.org/mediawiki/2014/1/15/CU_2014_OpenRoller_icon.png" title="The Cooper Union Open Tube Roller" height="100" /><br> |
| + | <span class="hardware"><a href="https://2014.igem.org/Team:Cooper_Union/OpenRoller">OpenRoller</a></span> |
| + | </div> |
| + | <div class="hardware"> |
| + | <img src="https://static.igem.org/mediawiki/2014/3/3e/CU_OpenFuge-stock.jpg" title="OpenFuge" height="100" /><br> |
| + | <span class="hardware"><a href="http://www.instructables.com/id/OpenFuge/" target="_blank">OpenFuge</a></span> |
| + | </div> |
| + | <div class="hardware"> |
| + | <img src="https://static.igem.org/mediawiki/2014/4/44/CU_Micro_Sketch1.JPG" title="Macro/Micro Fluidics" height="100" width="100" /><br> |
| + | <span class="hardware"><a href="https://2014.igem.org/Team:Cooper_Union/Microfluidics">Microfluidics</a></span> |
| + | </div></div> |
| | | |
- | == Build ==
| |
| | | |
- | Plans
| |
| | | |
- | (Need to post the dxf files) All the pieces are laser cut
| + | <br><br><h1>OpenGelBox</h1><br><br> |
| | | |
- | Assembly
| |
| | | |
- | The acrylic pieces must be assembled and glued together with acrylic cement.
| + | <div class="center"> |
| + | <img src="https://static.igem.org/mediawiki/2014/e/ef/CU_2014_OpenGel2.png" title="Open Source Midi Gelbox" height="450"/> |
| + | </div> |
| + | <br><br> |
| + | <div class="text"> |
| | | |
| + | The OpenGelBox is an easy-to-build midi gel box that can run up to 30 samples simultaneously. It is built using 6mm and 2mm acrylic sheets with a laser cut time of under a half hour. Previously, our lab would need to run several mini gels in series in order to analyze a large number of samples. With the OpenGelBox, we can more quickly analyze many samples while minimizing the use of consumable materials involved in gel electrophoresis. |
| | | |
- | == Gallery ==
| + | <h2>Design</h2> |
| | | |
- | (need to post more pictures of gelbox) | + | <h3>Plans</h3> |
| + | |
| + | <br> |
| + | <div class="hardware"> |
| + | <img src="https://static.igem.org/mediawiki/2014/6/6a/CU_OpenGel_CAD1.PNG" width="500" height="300" title="Cooper Union OpenGelBox" /><img src="https://static.igem.org/mediawiki/2014/9/97/CU_OpenGel_CAD2.PNG" width="500" height="300" title="Cooper Union OpenGelBox" /></div> |
| + | <br> |
| + | |
| + | <img src="https://static.igem.org/mediawiki/2014/7/74/CU_OpenGel-All_part_dwg2.png" width="350" /><br> |
| + | <a href="http://www.thingiverse.com/thing:501520">CAD Files</a> |
| + | <br><br> |
| + | <h3>Parts</h3> |
| + | <ul> |
| + | <li>6mm and 2mm acrylic sheet |
| + | <li>20x20mm acrylic square rod |
| + | <li>2 Male Banana plugs (or the your standardized plug) |
| + | <li>Platinum wire (AWG 30) |
| + | <li>Four M3x15 screws and nuts |
| + | <li>Acrylic glue |
| + | </ul> |
| + | <br><br> |
| + | <h3>Cut List</h3> |
| + | <br> |
| + | <dl><dt>6mm sheet</dt> |
| + | <dl> |
| + | <ul> |
| + | <li>Two “Sides”</li> |
| + | <li>One “base_plate”</li> |
| + | <li>Two “electrode_plate”</li> |
| + | <li>Two “front_back”</li> |
| + | <li>One “lid_top”</li> |
| + | <li>One “lid_electrode”</li> |
| + | <li>One “gel_base”</li> |
| + | </ul></dl> |
| + | <dt>2mm sheet</dt> |
| + | <ul><li>Two “Comb” </li> |
| + | <li>Two 20x20x190mm acrylic square rods</li> |
| + | </ul></dl></dt> |
| + | <br><br> |
| + | <h3>Assembly</h3> |
| + | <br> |
| + | <ol> |
| + | <li>Glue all plates, except the “electrode_plate”, together using acrylic glue. Clamps are recommended to ensure a watertight seal and better alignment.</li> |
| + | <li>Attach male banana plugs to holes in the “sides” plate</li> |
| + | <li>Attach the electrode wire to the power plugs and thread through the small hole in the “electrode_plate” so that the small hole is on the opposite side of the power plugs and the wire is behind the grate section</li> |
| + | <li>Screw both “electrode_plate” pieces to their respective sides using M3 screws</li> |
| + | <li>Notch two evenly spaced slots into the acrylic square rods, this will be where the combs sit. The notch depth depends on your gel thickness but should be around 9mm</li> |
| + | <li>Glue the square rods to the “gel_base” using the combs as alignment references</li> |
| + | <li>Glue lid pieces together and attach female banana plug</li> |
| + | </ol> |
| + | <br><br> |
| + | |
| + | |
| + | <div class="center"> |
| + | <div class="hardware"> |
| + | <img src="https://static.igem.org/mediawiki/2014/0/0b/CU_2014_OpenGel.png" title="The Cooper Union Open Midi Gel Box" height="250" /><br> |
| + | <span class="hardware">Open Source Midi Gel box</span> |
| + | </div> |
| + | <div class="hardware"> |
| + | <img src="https://static.igem.org/mediawiki/2014/c/c1/CU_2014_MidiCaster.png" title="The Cooper Union Open Midi Gel Box" height="250" /><br> |
| + | <span class="hardware">Gel caster</span> |
| + | </div> |
| + | <div class="hardware"> |
| + | <img src="https://static.igem.org/mediawiki/2014/d/db/CU_2014_MidiCaster2.png" title="The Cooper Union Open Midi Gel Box" height="250" /><br> |
| + | <span class="hardware">Gel caster and Comb</span> |
| + | </div> |
| + | </div> |
| + | <!-- |
| + | xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx |
| + | DO NOT EDIT BELOW HERE |
| + | xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx --> |
| + | </div> |
| + | |
| + | <br> |
| + | <br> |
| + | <hr> |
| + | |
| + | |
| + | </div> |
| + | |
| + | |
| + | |
| + | |
| + | <div class="cu-footer"> |
| + | <div class="bottom-left"> |
| + | <a href="https://2014.igem.org/Team:Cooper_Union" span="The Cooper Union: μToolbox"><img src="https://static.igem.org/mediawiki/2014/b/bd/CU_2014_logo.png" height="150" /></a></div> |
| + | <div class="bottom-center"> |
| + | <a href="https://2014.igem.org/Team:Cooper_Union/Project" class="black" > |
| + | <img src="https://static.igem.org/mediawiki/2014/2/27/CU_2014_LogoP.png" height="100" style="vertical-align: top;" title="Projects" /> |
| + | <span>Projects</span> |
| + | </a> |
| + | <a href="https://2014.igem.org/Team:Cooper_Union/Social" class="red" > |
| + | <img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Social" /> |
| + | <span>Social</span> |
| + | </a> |
| + | <a href="https://2014.igem.org/Team:Cooper_Union/Notebook" class="green" > |
| + | <img src="https://static.igem.org/mediawiki/2014/d/da/CU_2014_LogoN.png" height="100" style="vertical-align: top;" title="Notebook" /> |
| + | <span>Notebook</span> |
| + | </a> |
| + | <a href="https://2014.igem.org/Team:Cooper_Union/biofanfic"class="blue"> |
| + | <img src="https://static.igem.org/mediawiki/2014/3/38/CU_2014_LogoT.png" height="100" style="vertical-align: top;" title="Team" /> |
| + | <span>Team</span> |
| + | </a> |
| + | </div> |
| + | <div class="bottom-right"> |
| + | <a href="http://www.cooper.edu" target="_blank" span="The Cooper Union"><img src="https://static.igem.org/mediawiki/2014/9/90/CU_2014_Cooper_union_logo.png" height="150" /></a> |
| + | </div> |
| + | </div> |
| + | |
| + | |
| + | |
| + | |
| + | </body> |
| + | </html> |