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Revision as of 08:05, 18 July 2014 (view source) Amotz (Talk | contribs) (→Thursday 17th July) ← Older edit		Revision as of 08:06, 18 July 2014 (view source) Amotz (Talk | contribs) (→Thursday 17th July) Newer edit →
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	===3 - PCR and Electrophoresis===		===3 - PCR and Electrophoresis===
		+	[[File:Electrophoresis_17.07.2014.jpg|400px|right]]
	''by Arnaud & Mathias''		''by Arnaud & Mathias''
	PCR targeting with oligos IPS70 and IPS71.		PCR targeting with oligos IPS70 and IPS71.
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-	[[File:Electrophoresis_17.07.2014.jpg|400px|right]]
	#Molecular weight marker 1kb		#Molecular weight marker 1kb
-	#	+	#Empty
	#IPS70 and IPS71 (1)		#IPS70 and IPS71 (1)
	#IPS70 and IPS71 (2)		#IPS70 and IPS71 (2)

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Revision as of 08:06, 18 July 2014

Contents 1 Thursday 17th July 1.1 Lab work 1.1.1 1 - Preparation of supercompetent cells 1.1.2 2 - Bacterial Culture 1.1.3 3 - PCR and Electrophoresis

Thursday 17th July

Lab work

1 - Preparation of supercompetent cells

By Mathias & Romain

Strains used: MG1655 (x2), MG1655Z1 (x2).

Protocol

2 - Bacterial Culture

by Fabio and Maher

Strains used: J23119 from the concentrated colony made the 16th July

5uL of antibiotic in each tube.

3 - PCR and Electrophoresis

by Arnaud & Mathias

PCR targeting with oligos IPS70 and IPS71.

1. Molecular weight marker 1kb
2. Empty
3. IPS70 and IPS71 (1)
4. IPS70 and IPS71 (2)

Protocol

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