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Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0034 mRFP Assay
From 2014.igem.org
(Difference between revisions)
| Line 125: | Line 125: | ||
<div class="step-tile"> | <div class="step-tile"> | ||
<h2 class="step-title"> | <h2 class="step-title"> | ||
| - | Transformation | + | Double Transformation |
</h2> | </h2> | ||
</div> | </div> | ||
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</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | Ptrc-stem-asB0034-stem on pHN1257& | + | Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) each of 2.5µL to DH5&alph |
</div> | </div> | ||
</div> | </div> | ||
| Line 159: | Line 159: | ||
</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | Ptrc-stem-asB0034-stem(pHN1257)& | + | Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) with pHN1257_up_F Tm:60.1℃ & asB0034-R Tm:68.7℃ |
| - | + | ||
| - | + | ||
</div> | </div> | ||
</div> | </div> | ||
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</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | Ptrc-stem-asB0034-stem(pHN1257)& | + | Ptrc-stem-asB0034-stem(pHN1257) & R0040-B0034-E1010-B0015(pSB6A1) |
LB 2 mL | LB 2 mL | ||
for 3hours | for 3hours | ||
Revision as of 00:48, 18 October 2014
Notebook
Lab Documents
140910 asB0034&B0034 mRFP Assay
-
Start
-
Double Transformation
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) each of 2.5µL to DH5&alph -
Colony PCR
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) with pHN1257_up_F Tm:60.1℃ & asB0034-R Tm:68.7℃ -
Preculture
Ptrc-stem-asB0034-stem(pHN1257) & R0040-B0034-E1010-B0015(pSB6A1) LB 2 mL for 3hours Turbidity was controlled 0.1 -
Culture
LB 2 mL IPTG 20 µ or not for 11 hours, 13hours, 15hours, 17 hours -
Assay
fluoresence imageing 514 nm 610 filter -
Complete!
