Team:Cooper Union/Notebook/Biohack April

From 2014.igem.org

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<ul class="menu">
<ul class="menu">
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li>
-
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan</a> </li>
+
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan Timer</a> </li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Biohack_project">Biohacker Kit </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Biohack_project">Biohacker Kit </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Hardware">OpenSource Hardware </a> </li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Hardware">OpenSource Hardware </a> </li>
 +
<!--<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Modeling">Modeling </a> </li>-->
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Parts">BioBrick Parts </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Parts">BioBrick Parts </a></li>
-
<!--<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Modeling">Modeling </a> </li>-->
 
</ul>
</ul>
</li>
</li>
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<li class="menu-safety"> <a class="menu" href="https://igem.org/Safety/Safety_Form?team_id=1354">Safety</a> </li>
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<li class="menu-safety"> Social
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<li class="menu-outreach"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Outreach">Outreach </a></li>
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<ul class="menu">
 +
<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Safety">Safety</a> </li>
 +
<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Ethics">Ethics</a> </li>
 +
<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Outreach">Outreach </a></li>
 +
</ul>
 +
</li>
<li class="menu-notebook">  Notebook  
<li class="menu-notebook">  Notebook  
<ul class="menu">
<ul class="menu">
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     <br style="clear:both" />
     <br style="clear:both" />
</div>
</div>
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<pre>
 
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3/13/14
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<h3>3/13/14</h3>
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Digested pBR322 with HindIII.
+
<ul><li>Digested pBR322 with HindIII.</li>
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Double digested pBR322 with HindIII and EcoRI.
+
<li>Double digested pBR322 with HindIII and EcoRI.</li>
 +
</ul>
-
3/27/14
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<h3>3/27/14</h3>
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Ran digests from 3/13/14 on gel.  
+
<ul>
-
Excised gel fragments with plasmid in them.
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  <li>Ran digests from 3/13/14 on gel. </li>
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Purified gel fragments.
+
  <li>Excised gel fragments with plasmid in them.</li>
 +
  <li>Purified gel fragments.</li>
 +
</ul>
-
4/10/14
+
<h3>4/10/14</h3>
Phosphatase treated the digested and double digested pBR322 from 3/13/14.
Phosphatase treated the digested and double digested pBR322 from 3/13/14.
4/17/14
4/17/14
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Digested UV, HSP, and GFP g-blocks with HindIII and EcoRI.  
+
<ul>
-
Performed five ligations:
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  <li>Digested UV, HSP, and GFP g-blocks with HindIII and EcoRI. </li>
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pBR322 with UV
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  <li>Performed five ligations:
-
pBR322 with HSP
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<dl><dd>pBR322 with UV</dd>
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pBR322 with GFP
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<dd>pBR322 with HSP</dd>
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UV with GFP
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<dd>pBR322 with GFP</dd>
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HSP with GFP
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<dd>UV with GFP</dd>
 +
<dd>HSP with GFP</dd></dl>
 +
</li>
 +
</ul>
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4/24/14
 
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Purified UV+GFP and HSP+GFP ligations.
 
-
Digested the two ligations with HindIII.
 
-
Ligated each one into pBR322.
 
-
</pre>
+
<h3>4/24/14</h3>
 +
<ul>
 +
  <li>Purified UV+GFP and HSP+GFP ligations.</li>
 +
  <li>Digested the two ligations with HindIII.</li>
 +
  <li>Ligated each one into pBR322.</li>
 +
</ul>
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</div>
</div>
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</div>
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<br>
<br>
<br>
<br>
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<div class="cu-footer">
 
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<div class="bottom">
 
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<a href="https://2014.igem.org/Team:Cooper_Union" span="The Cooper Union: μToolbox"><img src="https://static.igem.org/mediawiki/2014/b/bd/CU_2014_logo.png" height="150"  /></a></div>
 
-
<div class="bottom-center"
 
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<a href="https://2014.igem.org/Team:Cooper_Union/Project" span="Projects"><img src="https://static.igem.org/mediawiki/2014/2/27/CU_2014_LogoP.png" height="100" style="vertical-align: top;" title="Projects" /></a>
 
-
<a href="https://igem.org/Safety/Safety_Form?team_id=1354" span="Safety"><img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Safety" /></a>
 
-
<a href="https://2014.igem.org/Team:Cooper_Union/Outreach" span="Outreach"><img src="https://static.igem.org/mediawiki/2014/7/74/CU_2014_logoO.png" height="100" style="vertical-align: top;" title="Outreach" /></a>
 
-
<a href="https://2014.igem.org/Team:Cooper_Union/Notebook" span="Notebook"><img src="https://static.igem.org/mediawiki/2014/d/da/CU_2014_LogoN.png" height="100" style="vertical-align: top;" title="Notebook" /></a>
 
-
<a href="https://2014.igem.org/Team:Cooper_Union/biofanfic" span="Team"><img src="https://static.igem.org/mediawiki/2014/3/38/CU_2014_LogoT.png" height="100" style="vertical-align: top;" title="Team" /></a></div>
 
-
<div class="bottom-right">
 
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<a href="http://www.cooper.edu" target="_blank" span="The Cooper Union"><img src="https://static.igem.org/mediawiki/2014/9/90/CU_2014_Cooper_union_logo.png" height="150" /></a>
 
-
</div>
 
 +
<div class="cu-footer">
 +
    <div class="bottom-left">
 +
        <a href="https://2014.igem.org/Team:Cooper_Union" span="The Cooper Union: μToolbox"><img src="https://static.igem.org/mediawiki/2014/b/bd/CU_2014_logo.png" height="150"  /></a></div>
 +
    <div class="bottom-center">
 +
        <a href="https://2014.igem.org/Team:Cooper_Union/Project" class="black" >
 +
            <img src="https://static.igem.org/mediawiki/2014/2/27/CU_2014_LogoP.png" height="100" style="vertical-align: top;" title="Projects" />
 +
            <span>Projects</span>
 +
        </a>
 +
        <a href="https://2014.igem.org/Team:Cooper_Union/Social" class="red" >
 +
            <img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Social" />
 +
            <span>Social</span>
 +
        </a>
 +
        <a href="https://2014.igem.org/Team:Cooper_Union/Notebook" class="green" >
 +
            <img src="https://static.igem.org/mediawiki/2014/d/da/CU_2014_LogoN.png" height="100" style="vertical-align: top;" title="Notebook" />
 +
            <span>Notebook</span>
 +
        </a>
 +
        <a href="https://2014.igem.org/Team:Cooper_Union/biofanfic"class="blue">
 +
            <img src="https://static.igem.org/mediawiki/2014/3/38/CU_2014_LogoT.png" height="100" style="vertical-align: top;" title="Team" />
 +
            <span>Team</span>
 +
        </a>
 +
    </div>
 +
    <div class="bottom-right">
 +
        <a href="http://www.cooper.edu" target="_blank" span="The Cooper Union"><img src="https://static.igem.org/mediawiki/2014/9/90/CU_2014_Cooper_union_logo.png" height="150" /></a>
 +
    </div>
</div>
</div>

Latest revision as of 00:44, 18 October 2014

Cooper Union 2014 iGEM

Biohacker Kit

PRE MAY MAY JUN JUL


3/13/14

  • Digested pBR322 with HindIII.
  • Double digested pBR322 with HindIII and EcoRI.

3/27/14

  • Ran digests from 3/13/14 on gel.
  • Excised gel fragments with plasmid in them.
  • Purified gel fragments.

4/10/14

Phosphatase treated the digested and double digested pBR322 from 3/13/14. 4/17/14
  • Digested UV, HSP, and GFP g-blocks with HindIII and EcoRI.
  • Performed five ligations:
    pBR322 with UV
    pBR322 with HSP
    pBR322 with GFP
    UV with GFP
    HSP with GFP

4/24/14

  • Purified UV+GFP and HSP+GFP ligations.
  • Digested the two ligations with HindIII.
  • Ligated each one into pBR322.


Projects Social Notebook Team

Retrieved from "http://2014.igem.org/Team:Cooper_Union/Notebook/Biohack_April"