Team:Peking/Parts

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<li><a href="https://2014.igem.org/Team:Peking/Parts#parts01">Favorite Parts</a></li>
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<li><a href="https://2014.igem.org/Team:Peking/Parts#parts02">Part List</a></li>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Peking/Parts&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<h2 id="parts01">Favorite Parts</h2>
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<p><b>INPNC-Linker (rTEV)-MVN-mRFP</b></p>
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<p><a href="http://partsregistry.org/Part:BBa_K1378005" target="_blank">Main Page-BBa_K1378005</a>
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<p>BBa_K1378005 is a composite part coding INPNC-Linker (rTEV)-MVN-mRFP fusion protein. We confirmed that fusion protein is truly displayed outwards by using the INPNC displaying system by  rTEV protease digestion assay method and thus help characterize INPNC part.</p>
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<p><a href="https://2014.igem.org/Team:Peking/KillingImprovements" target="_blank">Visit Wiki</a>
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<p><b>MlrA</b></p>
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<p><a href="http://partsregistry.org/Part:BBa_K1378001" target="_blank">Main Page-BBa_K1378001</a>
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<p>MlrA is a protease which can cleavage Microcystin(MC) and and thus reduce its toxicity. We tested it in <i>E.coli</i> and showed that MlrA can decrease the concentration of MC and weaken its inhibitory activity to protein phosphatase 1.</p>
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<p><a href="https://2014.igem.org/Team:Peking/Degradation" target="_blank">Visit Wiki</a>
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<p><b>INPNC-MVN & GFP</b></p>
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<p><a href="http://partsregistry.org/Part:BBa_K1378007" target="_blank">Main Page-BBa_K1378007</a>
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<p>BBa_K1378007 is a composite part coding INPNC-MVN fusion protein and GFP. <i>E.coli</i> carrying this plasmid will display MVN on the cell surface and bind to <i>M. aeruginosa</i> as well as expression GFP, thus the binding phenomenon can be easily observed.</p>
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<p><a href="https://2014.igem.org/Team:Peking/KillingImprovements" target="_blank">Visit Wiki</a>
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<h2 id="parts02" style="margin-top:20px;">Part List</h2>
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<h3>Parts for Killing Improvement</h3>
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<p><a href="http://partsregistry.org/Part:BBa_K1378003" target="_blank">BBa_K1378003</a> is the coding sequence of lectin Microvirin.</p>
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<p><a href="http://partsregistry.org/Part:BBa_K1378005" target="_blank">BBa_K1378005</a> is the coding sequence of INPNC-MVN-mRFP fusion protein.</p>
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<p><a href="http://partsregistry.org/Part:BBa_K1378007" target="_blank">BBa_K1378007</a> is a composite part of GFP and INPNC-MVN fusion protein.</p>
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<p><a href="http://partsregistry.org/Part:BBa_K1378009" target="_blank">BBa_K1378009</a> is a composite part of GFP and INPNC-MVN fusion protein with a linker between INPUC and MVN which can be cleavaged by rTEV.</p>
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<h3>Parts for Degradation</h3>
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<p><a href="http://partsregistry.org/Part:BBa_K1378001" target="_blank">BBa_K1378001</a> is protease MlrA which can specifically cleavage Microcystin(MC).</p>
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<p><a href="http://partsregistry.org/Part:BBa_K1378022" target="_blank">BBa_K1378022</a> is the coding sequence of MlrA fused with PelB secretion tag.</p>
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<p><a href="http://partsregistry.org/Part:BBa_K1378023" target="_blank">BBa_K1378023</a> is the coding sequence of MlrA fused with TorA secretion tag.</p>
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<p><a href="http://partsregistry.org/Part:BBa_K1378024" target="_blank">BBa_K1378024</a> is the coding sequence of MlrA with terminator.</p>
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<p><a href="http://partsregistry.org/Part:BBa_K1378025" target="_blank">BBa_K1378025</a> is the coding sequence of PelB-MlrA fusion protein with terminator.</p>
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<p><a href="http://partsregistry.org/Part:BBa_K1378026" target="_blank">BBa_K1378026</a> is the coding sequence of TorA-MlrA fusion protein with terminator.</p>
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<br/><br/>
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<h3>Parts for Suicide</h3>
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<p><a href="http://partsregistry.org/Part:BBa_K1378031" target="_blank">BBa_K1378031</a> is the coding sequence for holin.</p>
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<p><a href="http://partsregistry.org/Part:BBa_K1378032" target="_blank">BBa_K1378032</a> is the coding sequence for endolysin.</p>
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<a href="https://2014.igem.org/Team:Peking"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:Peking/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Peking"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:Peking/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:Peking/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:Peking/Attributions"style="color:#000000"> Attributions </a></td>
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<td > <h3>What information do I need to start putting my parts on the Registry? </h3></td>
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will submit them to the <a href="http://partsregistry.org"> Registry of Standard Biological Parts</a>. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox. 
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<strong>Note that if you want to document a part you need to document it on the <a href="http://partsregistry.org Registry"> Registry</a>, not on your team wiki.</strong> Future teams and other users and are much more likely to find parts on the Registry than on your team wiki.
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Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
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<h3>When should you put parts into the Registry?</h3>
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As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements.
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The information needed to initially create a part on the Registry is:
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part <a href="http://parts.igem.org/Part:BBa_K404003">BBa_K404003</a> for an excellent example of a highly characterized part.
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You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry"> Add a Part to the Registry</a> link.
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<tr><td colspan="3" > <h3> Parts Table</h3></td></tr>
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Any parts your team has created will appear in this table below:</td></tr>
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<groupparts>iGEM013 Peking</groupparts>
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Latest revision as of 00:44, 18 October 2014

Favorite Parts

INPNC-Linker (rTEV)-MVN-mRFP

Main Page-BBa_K1378005

BBa_K1378005 is a composite part coding INPNC-Linker (rTEV)-MVN-mRFP fusion protein. We confirmed that fusion protein is truly displayed outwards by using the INPNC displaying system by rTEV protease digestion assay method and thus help characterize INPNC part.

Visit Wiki

MlrA

Main Page-BBa_K1378001

MlrA is a protease which can cleavage Microcystin(MC) and and thus reduce its toxicity. We tested it in E.coli and showed that MlrA can decrease the concentration of MC and weaken its inhibitory activity to protein phosphatase 1.

Visit Wiki

INPNC-MVN & GFP

Main Page-BBa_K1378007

BBa_K1378007 is a composite part coding INPNC-MVN fusion protein and GFP. E.coli carrying this plasmid will display MVN on the cell surface and bind to M. aeruginosa as well as expression GFP, thus the binding phenomenon can be easily observed.

Visit Wiki


Part List

Parts for Killing Improvement

BBa_K1378003 is the coding sequence of lectin Microvirin.

BBa_K1378005 is the coding sequence of INPNC-MVN-mRFP fusion protein.

BBa_K1378007 is a composite part of GFP and INPNC-MVN fusion protein.

BBa_K1378009 is a composite part of GFP and INPNC-MVN fusion protein with a linker between INPUC and MVN which can be cleavaged by rTEV.



Parts for Degradation

BBa_K1378001 is protease MlrA which can specifically cleavage Microcystin(MC).

BBa_K1378022 is the coding sequence of MlrA fused with PelB secretion tag.

BBa_K1378023 is the coding sequence of MlrA fused with TorA secretion tag.

BBa_K1378024 is the coding sequence of MlrA with terminator.

BBa_K1378025 is the coding sequence of PelB-MlrA fusion protein with terminator.

BBa_K1378026 is the coding sequence of TorA-MlrA fusion protein with terminator.



Parts for Suicide

BBa_K1378031 is the coding sequence for holin.

BBa_K1378032 is the coding sequence for endolysin.

Retrieved from "http://2014.igem.org/Team:Peking/Parts"