Team:NTU Taida/SAFETY
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<p style="padding-bottom:30px"><strong>This</strong> is the gene circuit of self-destroying system. The PKEK coding sequence can be substituted by any other target gene. As a result, this system can be applied by many other iGEM team.</p> | <p style="padding-bottom:30px"><strong>This</strong> is the gene circuit of self-destroying system. The PKEK coding sequence can be substituted by any other target gene. As a result, this system can be applied by many other iGEM team.</p> | ||
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<strong>K117000</strong> coding region precedes a repressible promoter <a href="http://parts.igem.org/Part:BBa_R0011">http://parts.igem.org/Part:BBa_R0011</a>, which is a highly registered biobrick. The lysis gene is efficiently inhibited by LacI protein originally. Once the uptake of fatty acid occurred, the pLacI promoter will be activated, causing the expression of K117000. The dynamic response of K117000 gene is delayed comparing to the activation of target gene (PKEK) due to the latency incurred by the additional components in the system. As a result, the plasmid containing bacteria will die after the target protein expresses for a while. This prediction of circuit property can be supported by the article, “Parameter Estimation For Two Synthetic Gene Networks: A Case Study”.</p> | <strong>K117000</strong> coding region precedes a repressible promoter <a href="http://parts.igem.org/Part:BBa_R0011">http://parts.igem.org/Part:BBa_R0011</a>, which is a highly registered biobrick. The lysis gene is efficiently inhibited by LacI protein originally. Once the uptake of fatty acid occurred, the pLacI promoter will be activated, causing the expression of K117000. The dynamic response of K117000 gene is delayed comparing to the activation of target gene (PKEK) due to the latency incurred by the additional components in the system. As a result, the plasmid containing bacteria will die after the target protein expresses for a while. This prediction of circuit property can be supported by the article, “Parameter Estimation For Two Synthetic Gene Networks: A Case Study”.</p> |
Latest revision as of 00:43, 18 October 2014