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- | <a href="https://2014.igem.org/Team:USTC-China/project/killswitch" class="active-a">Kill switch</a> | + | <a href="https://2014.igem.org/Team:USTC-China/project/killswitch" class="active-a">Kill Switch</a> |
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| <div class="large-9 columns" id="content-page"> | | <div class="large-9 columns" id="content-page"> |
- | <div class="title"><h1>Kill Switch</h1></div> | + | <div class="title"><h1>Kill Switch</h1> |
| + | <div data-magellan-expedition="fixed"> |
| + | <dl class="sub-nav"> |
| + | <dd data-magellan-arrival="background"><a href="#background">Background</a></dd> |
| + | <dd data-magellan-arrival="principle"><a href="#principle">Principle</a></dd> |
| + | </dl> |
| + | </div> |
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| <div class="text"> | | <div class="text"> |
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- | <h4 id="background">Background</h4> | + | <a name="background"></a> |
| + | <h4 data-magellan-destination="background">Background</h4> |
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| <p>To ensure that our bacteria do no harm to both people and environment, we need to put a set of kill-switch system into the bacteria, so that we can control the growth of them by changing the contents of culture medium.</p> | | <p>To ensure that our bacteria do no harm to both people and environment, we need to put a set of kill-switch system into the bacteria, so that we can control the growth of them by changing the contents of culture medium.</p> |
| <p>In our project, we trigger the kill switch of <i>E.coli</i> by adding <i>IPTG</i> into the culture. <i>IPTG</i> can induce the expression of gene coding <i>Limulus anti-LPS factor</i> (<i>LALF</i>) downstream the lactose operator, so that the growth of <i>E.coli</i> will be inhibited.<p/> | | <p>In our project, we trigger the kill switch of <i>E.coli</i> by adding <i>IPTG</i> into the culture. <i>IPTG</i> can induce the expression of gene coding <i>Limulus anti-LPS factor</i> (<i>LALF</i>) downstream the lactose operator, so that the growth of <i>E.coli</i> will be inhibited.<p/> |
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- | <h4 id="principle">Principle</h4> | + | <a name="principle"></a> |
| + | <h4 data-magellan-destination="principle">Principle</h4> |
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| <p>Limulus <i>anti-LPS</i> factor (<i>LALF</i>) binds <i>LPS</i> on anti-gram negative bacterial surface tightly, forming a biofilm. This can prevent nutrient from entering the cell, causing the growth inhibition. Also, the biofilm can prevent plasmid spreading to the environment and gene pollution.</p> | | <p>Limulus <i>anti-LPS</i> factor (<i>LALF</i>) binds <i>LPS</i> on anti-gram negative bacterial surface tightly, forming a biofilm. This can prevent nutrient from entering the cell, causing the growth inhibition. Also, the biofilm can prevent plasmid spreading to the environment and gene pollution.</p> |
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| <p><div align="center"><img src="https://static.igem.org/mediawiki/2014/d/da/LAFLG_MEch.jpg" class="th"/></div></p> | | <p><div align="center"><img src="https://static.igem.org/mediawiki/2014/d/da/LAFLG_MEch.jpg" class="th"/></div></p> |
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- | <h4 id="results">Results</h4>
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- | <p>We constructed the plasmid with lactose operator and signal peptide, and transformed this plasmid into <i>E.coli</i> to test if this kill switch do works. We used gradient plate method in our experiment. As you can see in the picture, the concentration of <i>IPTG</i> in the left of the medium is 0.1% while the concentration on the right is zero. We can see colony only on the right of the medium. That kill switch is effective illustrates that <i>IPTG</i> can induce the expression of signal peptide so as to inhibit the growth of the <i>E.coli</i>. Also, a decrease of OD is observed after we added <i>IPTG</i> to the liquid culture medium. </p>
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- | <p><div align="center"><img style="height: 450px;" src="https://static.igem.org/mediawiki/2014/8/87/Sdfsdfs.jpg" class="th"/></div></p>
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- | <p>However, we haven’t connect the <i>LALF</i> gene with signal peptide yet due to the time limit. We will construct the whole plasmid and transform it into <i>E.coli</i>. to see the sterilization effect of <i>LALF</i> in the future work.<p/>
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| </div> | | </div> |
| </div> | | </div> |